A kind of thermosensitive gel material combination and its preparation method and application

A temperature-sensitive gel and gel technology, applied in the field of regenerative medicine, can solve the problems of increased cost and high requirements of 3D printing technology, and achieve the effect of promoting regeneration

Active Publication Date: 2022-07-29
THE FIRST AFFILIATED HOSPITAL OF JINAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in order to ensure the adaptability of defects in different situations, the stent needs to be precisely designed according to the actual situation, which will increase additional costs; moreover, the complicated stent manufacturing process and often requires invasive stent implantation, making 3D printing high technical requirements

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  • A kind of thermosensitive gel material combination and its preparation method and application
  • A kind of thermosensitive gel material combination and its preparation method and application
  • A kind of thermosensitive gel material combination and its preparation method and application

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preparation example Construction

[0042] The present invention also provides a preparation method of the temperature-sensitive gel material combination, comprising the following steps:

[0043] (1) mixing chitosan with acetic acid solution to obtain chitosan solution;

[0044] (2) β-glycerophosphate disodium and hyaluronic acid are mixed and dissolved in water to obtain a hyaluronic acid solution;

[0045] (3) mixing chitosan solution and hyaluronic acid solution to obtain chitosan hyaluronic acid solution;

[0046] (4) mixing SDF-1 with a 40-60% volume chitosan hyaluronic acid solution to obtain a chitosan hyaluronic acid solution loaded with SDF-1;

[0047] (5) Mix 40-60% volume chitosan hyaluronic acid solution with 1-ethyl-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide, then add Apt19S aptamer to obtain Apt19S-loaded chitosan hyaluronic acid solution.

[0048] In the present invention, chitosan is mixed with acetic acid solution to obtain chitosan solution.

[0049] In the present inventio...

Embodiment 1

[0092] 48 mg of chitosan (CS) was dissolved in 1.6 mL of 0.1 M acetic acid solution, placed on a magnetic stirrer and stirred continuously for 2 h (1000 rpm), and sterilized by autoclaving at 121° C. for 10 min to obtain the chitosan solution. 240 mg of β-GP and 4 mg of hyaluronic acid were dissolved in 0.4 mL of deionized water, and sterilized by filtration with a 0.22 μm filter to obtain a dissolved hyaluronic acid solution. Chitosan solution and hyaluronic acid solution were ice-bathed (0°C) for 15min, then hyaluronic acid was added dropwise to the chitosan solution and stirred with a magnetic stirrer for 10min (1000rpm) to obtain a chitosan hyaluronic acid solution . Divide the chitosan hyaluronic acid solution into two equal parts, 1 mL each.

[0093] Take 1 mL of chitosan hyaluronic acid solution, dropwise add 1 μl of SDF-1 solution with a concentration of 100 μg / mL dissolved in PBS, and stir magnetically for 10 min (1000 rpm) to obtain a loaded SDF with a SDF-1 concent...

Embodiment 2

[0098] 100 μL of the third-generation rBMSCs culture medium (containing 1×10 3 cells) were seeded into the upper chamber of the Transwell chamber, and 600 μL of α-MEM medium containing 200 ng / mL SDF-1 and 600 μL of α-MEM medium containing amino-modified Apt19S with a concentration of 2 nmol / mL were added to the lower chamber of Transwell. at 37°C, CO 2 After culturing in an incubator with a concentration of 5% for 24 hours, the unmigrated cells in the upper chamber were wiped off with a cotton swab. The cells that migrated to the lower chamber were fixed with 4% formaldehyde for 15 minutes, rinsed twice with PBS, air-dried, and 0.5% crystal violet for 30 minutes. Rinse 3 times with PBS. Then, 5 fields of view were randomly selected from each chamber under the microscope, and the BMSC cells that migrated to the lower chamber were counted. The results showed that the addition of SDF-1 and amino-modified Apt19S aptamer could significantly promote the migration of BMSC cells com...

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Abstract

The invention provides a temperature-sensitive hydrogel material combination and a preparation method and application thereof, belonging to the technical field of regenerative medicine. The temperature-sensitive gel material combination provided by the present invention includes SDF-1-loaded chitosan-hyaluronic acid solution and Apt19S-loaded chitosan-hyaluronic acid solution. The present invention aims to develop a thermosensitive injectable bilayer hydrogel loaded with biological factors related to cell migration, so as to promote the regeneration of periodontal tissue.

Description

technical field [0001] The invention relates to the technical field of regenerative medicine, in particular to a temperature-sensitive gel material combination and a preparation method and application thereof. Background technique [0002] Chronic periodontitis is a common clinical chronic inflammatory disease with progressive destruction of periodontal support tissue as the main pathological change. In severe cases, it can lead to tooth loosening or even loss. The existing supragingival scaling, subgingival scaling and other treatment methods can only control the progression of the disease, but cannot reconstruct the destroyed periodontal tissue. Therefore, many scholars expect to use tissue engineering to achieve periodontal tissue regeneration, providing a new idea for the treatment of chronic periodontitis. [0003] As one of the three elements of tissue engineering, seed cells are the basic conditions for tissue regeneration. However, the use of allogeneic cells in vi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/22A61L27/54A61L27/52A61L27/50
CPCA61L27/20A61L27/227A61L27/54A61L27/52A61L27/50A61L2430/12A61L2430/34A61L2300/252A61L2300/412A61L2400/06C08L5/08C08L89/00A61L2300/414A61L27/48A61L27/56
Inventor 刘湘宁蔡明详许梓楠梁言
Owner THE FIRST AFFILIATED HOSPITAL OF JINAN UNIV
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