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Method for detecting dermatophyte

A dermatophyte and detection time technology, applied in the preparation of test samples, measuring devices, instruments, etc., can solve problems such as affecting the efficiency of fungal detection, difficulty in finding detection methods, etc. The effect of improving the positive detection rate

Pending Publication Date: 2022-03-25
江西业力医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the existing fungus detection when making glass slides, there are multiple processing methods, it is difficult to find the best detection method, and the shortcomings of affecting the efficiency of fungal detection, and a method for skin fungus detection proposed

Method used

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  • Method for detecting dermatophyte

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] refer to figure 1 , a method for skin fungus detection, comprising the following steps:

[0027] S1: Take skin scrapings, nail scraps or hair samples to make samples;

[0028] S2: grind the sample through a homogenizer, mix and centrifuge;

[0029] S3: Discard the supernatant, take sediment smears or use automatic equipment to make slices, and label them;

[0030] S4: Add fungal fluorescent staining solution to the labeled sample slides, and record the color development time;

[0031] S5: Obtain the average color development time of the labeled sample slide, record it as the detection time, and compare multiple detection times;

[0032] S6: Determine the best inspection method according to the comparison results.

[0033] In this example, in S1, samples of skin flakes, nail flakes or hair were taken and stored in a sample treatment solution and left to stand for 3 minutes at 37°C to prepare samples. The sample treatment solution could facilitate the removal of fungi...

Embodiment 2

[0041] refer to figure 1 , a method for skin fungus detection, comprising the following steps:

[0042] S1: Take skin scrapings, nail scraps or hair samples to make samples;

[0043] S2: grind the sample through a homogenizer, mix and centrifuge;

[0044] S3: Discard the supernatant, take sediment smears or use automatic equipment to make slices, and label them;

[0045] S4: Add fungal fluorescent staining solution to the labeled sample slides, and record the color development time;

[0046] S5: Obtain the average color development time of the labeled sample slide, record it as the detection time, and compare multiple detection times;

[0047] S6: Determine the best inspection method according to the comparison results.

[0048] In this example, in S1, samples of skin flakes, nail flakes or hair are taken and stored in a sample treatment solution and left to stand at 44°C for 6 minutes to make samples. The sample treatment solution can facilitate the removal of fungi from ...

Embodiment 3

[0056] refer to figure 1 , a method for skin fungus detection, comprising the following steps:

[0057] S1: Take skin scrapings, nail scraps or hair samples to make samples;

[0058] S2: grind the sample through a homogenizer, mix and centrifuge;

[0059]S3: Discard the supernatant, take sediment smears or use automatic equipment to make slices, and label them;

[0060] S4: Add fungal fluorescent staining solution to the labeled sample slides, and record the color development time;

[0061] S5: Obtain the average color development time of the labeled sample slide, record it as the detection time, and compare multiple detection times;

[0062] S6: Determine the best inspection method according to the comparison results.

[0063] In this example, in S1, samples of dander, nail flakes or hair are taken and stored in a sample treatment solution and left to stand at 50°C for 10 minutes to prepare samples. The sample treatment solution can facilitate the removal of fungi from da...

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Abstract

The invention belongs to the field of fungus detection, particularly relates to a method for detecting dermatophyte, and aims to solve the problems that multiple treatment methods exist, an optimal detection method is difficult to find and fungus detection efficiency is affected when a slide is manufactured in existing fungus detection, the following scheme is provided: the method comprises the following steps: S1, taking a scurf sample, a nail scurf sample or a hair sample to manufacture a sample; s2, grinding the sample through a homogenizer, uniformly mixing and performing centrifugal treatment; s3, discarding the supernate, taking the precipitate to smear or preparing a sheet by using automatic equipment, and labeling; s4, dropwise adding a fungus fluorescent staining solution into the labeled sample slide, and recording the color developing time; s5, obtaining the average color developing time of the labeled sample slides, recording the average color developing time as detection time, and comparing a plurality of detection times; according to the invention, a plurality of detection times are compared, so that the shortest detection time can be found conveniently, and an optimal detection method can be obtained.

Description

technical field [0001] The invention relates to the technical field of fungus detection, in particular to a method for skin fungus detection. Background technique [0002] Fungi are a kind of eukaryotic organisms. According to their growth characteristics and morphological differences, fungi can be simply divided into yeast, fungi and mushrooms (mushrooms). Among them, there are about 300 kinds of fungi that are pathogenic to humans, and almost all of the pathogenic fungi that are medically meaningful are molds. Clinically, fungal infections are divided into superficial fungal infections and deep fungal infections according to the different parts of the human body that are invaded. Among them, superficial fungal infection is a disease caused by fungi invading the skin, hair and nails of the human body. Deep fungal infection is fungal invasion of human mucosa, deep tissue and internal organs, and even cause systemic disseminated infection. Among them, deep fungal infection...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N1/38G01N1/30G01N1/28
CPCG01N21/6428G01N21/6486G01N1/286G01N1/38G01N1/30
Inventor 熊文芳吕正和陶根金袁书娟方继利程菊
Owner 江西业力医疗器械有限公司