Full-automatic immunoblotting instrument and use method thereof

Abstract

The invention discloses a full-automatic immunoblotting instrument and a using method thereof.The full-automatic immunoblotting instrument comprises a rack, a sample module, a uniform mixing module, a liquid adding and absorbing module, an air drying module, a sample adding module and an image collecting module, the rack comprises a rack body, a door, a first side plate, a second side plate and a top plate, and the door is installed on the rack body; a sample module, a uniform mixing module, a liquid adding and absorbing module, an air drying module, a sample adding module and an image acquisition module are mounted in the rack main body. According to the invention, full-automatic sample adding and liquid adding, film strip air drying and result interpretation can be realized, manpower is saved, the device is more convenient, and automatic treatment is realized; the noise during treatment is reduced, the cost rise caused by using a high-power fan is avoided, and the phenomenon that the film strip is blown back or away from the correct position is also avoided; the film groove can always face the fan, so that the air drying efficiency is improved; more stable opening and closing of the door are realized; and the recovery of the TIP head is realized.

Description

technical field [0001] The invention relates to an immunoblotting instrument, in particular to an automatic immunoblotting instrument and a method for using the same. Background technique [0002] In western blotting, proteins are transferred to a membrane and then detected using antibodies. The corresponding antibody can be used as the primary antibody to detect the known expressed protein, and the expression product of the new gene can be detected by the antibody of the fusion part. Western blotting has the advantages of large analysis capacity, high sensitivity, and strong specificity. It is the most commonly used method for detecting protein characteristics, expression, and distribution, such as qualitative and quantitative detection of tissue antigens, mass determination of polypeptide molecules, and detection of viruses. Antibody or antigen detection, etc. [0003] At present, the detection method is mainly based on manual operation or semi-automatic Western blotting...

AI Technical Summary

Problems solved by technology

The addition of serum, the air-drying of the membrane strips and the interpretation of the results still need to be done manually, which is time-consuming and laborious, and the operation is inconvenient, and automatic processing cannot be realized. Sometimes it is necessary to place the film strip horizontally to dry the film strip with a fan, which results in loud noise, requires a higher power fan, increases the cost of processing, and also has the phenomenon of blowing the film strip backwards or away from the correct position. ; What's more, most of the doors of the existing western blotting equipment are left and right push-pull or unilaterally rotated to connect the shaft type opening and closing, which has caused problems such as large space occupation, heavy weight, and high noise; and, the current immunoblot It is difficult for the imprinter to recycle the TIP head (pipette tip). At present, the way to collect the waste TIP head on similar equipment is to slide the used TIP head into the trash can along the pipeline after being discarded through the pipetting module. , but there are pipelines in this method in practical application, because the instrument is placed on the workbench and the pipeline cannot be installed, so it is difficult to collect the waste TIP head

Images