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Microbial composition with weight-losing effect and application thereof

A microbial composition and obesity technology, applied in the field of microorganisms, can solve problems such as changes in the microbiota of the recipient host, and achieve the effect of improving abnormal body weight

Active Publication Date: 2022-03-29
SUZHOU PRECISION BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the invention has room for further optimization for the compounding of strains
[0007] In addition, although there have been many reports on the use of AKK bacteria for weight loss, the prior art also pointed out that the gut microbiota is involved in various diseases, including colon cancer, depression and obesity, etc., which suggests that fecal microbiota transplantation may lead to Altered host microbiota, putting them at greater risk in these conditions

Method used

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  • Microbial composition with weight-losing effect and application thereof
  • Microbial composition with weight-losing effect and application thereof
  • Microbial composition with weight-losing effect and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1 The cultivation of Lactobacillus gasseri and Akkermansia muciniphila

[0067] This example provides the cultivation of Lactobacillus gasseri and Akkermansia muciniphila in the present invention.

[0068] (1) Cultivation of Lactobacillus gasseri

[0069] Strain activation: inoculate Lactobacillus gasseri used in the present invention (Lactobacillus gasseri LG08 of Jiangsu Weikang Biotechnology Co., Ltd., stored on a slant) into 50 mL of MRS medium, culture in a shaker flask at 37°C and 150 rotations for 24 hours, and obtain seeds liquid.

[0070] Fermentation culture: inoculate the activated seed liquid into 1L MRS medium according to 1% inoculum amount (volume ratio), and culture in a shaker flask at 37° C. at 150 rotations for 48 hours.

[0071] The MRS medium formula is as follows:

[0072] Element Content (g / L) Peptone 10.0 beef noodles 8.0 yeast 4.0 glucose 20.0 Dipotassium phosphate 2.0 Diammonium hydrog...

Embodiment 2

[0083] Embodiment 2 mouse model test

[0084] In this example, the in vivo test of the microbial single bacterium and microbial mixture in the implementation case 1 in a high-fat diet-induced obesity mouse model verifies its use in the treatment or prevention of obesity, and its single bacterium drug effect and mixed bacterium drug effect for comparison.

[0085] experimental method:

[0086] (1) After centrifuging the cultured Lactobacillus gasseri and Akkermans mucini bacteria and discarding the supernatant, use sterile PBS to adjust the concentration of the bacteria solution to 1×10 9 CFU / mL, the mice were gavaged according to the following groups:

[0087] Lactobacillus gasseri alone;

[0088] Mucin-Akkermansia alone;

[0089] Lactobacillus gasseri and mucin-Akkermansia mixed at a ratio of 1:1;

[0090] The volume of intragastric administration in each group was 0.2 mL, once a day.

[0091] (2) Animal experiments: 30 10-week-old C57BL / 6 female mice (purchased from Na...

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Abstract

The invention provides a microbial composition with a weight losing effect. The microbial composition is prepared from lactobacillus gasseri and ackermansiella muciniphila, wherein the lactobacillus gasseri and the ackermansiella muciniphila are added into the microbial composition; the ratio of the viable count of the lactobacillus gasseri to the viable count of the mucophilic protein-ackermania is (2: 3)-(3: 2). By means of the probiotic composition containing lactobacillus gasseri and mucoprotein-ackermania in a specific ratio and specific content, the condition of abnormal body weight caused by metabolic diseases can be improved.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a combination of microbes with weight loss effect and application thereof. Background technique [0002] The types and numbers of microbial flora in the human intestinal tract are extremely large. The intestinal tract is not only the largest digestive organ in the body, but also an organ that integrates endocrine, immune and barrier functions. Under normal physiological conditions, the flora in the intestinal tract is in a dynamic balance, helping the intestinal tract to digest and absorb food, and maintaining the normal metabolism of the body. [0003] Lactobacillus gasseri (Lactobacillus gasseri) is a species of Lactobacillus and one of the more common strains in the human body. Studies have shown that Lactobacillus gasseri can reduce the waist circumference, body mass index (BMI) and visceral fat of the human body. Has a good weight loss effect; Akkermansia muciniphila (AKK for short)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P3/04A61P9/12A61P3/06A61P3/10A61P1/16A61P19/06A61P19/10A61P9/10A23L33/135A23K10/18C12R1/225C12R1/01A61K35/74
CPCC12N1/20A61K35/747A61K35/74A61P3/04A61P9/12A61P3/06A61P3/10A61P1/16A61P19/06A61P19/10A61P9/10A23L33/135A23K10/18A61K2300/00Y02A50/30
Inventor 朱永亮穆晓静朱蒙蒙张叶静徐青青
Owner SUZHOU PRECISION BIOTECH CO LTD
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