Sweet potato anthocyanin related SNP (Single Nucleotide Polymorphism) molecular marker, identification and application thereof
A technology of molecular markers and anthocyanins, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of long breeding process and large consumption of manpower and material resources, so as to save breeding time and cost The effect of less and less data volume
Active Publication Date: 2022-04-01
CROP RES INST SHANDONG ACAD OF AGRI SCI
View PDF6 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Breeding purple sweet potato varieties needs to germinate the seeds obtained by sexual hybridization in the seedbed first, and then transplant them into the field. Only after the potatoes are formed can we know whether they are purple sweet potatoes. The breeding process takes a long time and consumes a lot of manpower and material resources.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0037] SNaPshot technology experimental method
[0038] 1. DNA extraction
[0039] Use TSINGKE Plant DNA Extraction Kit (Universal), the specific steps are as follows:
[0040] 1.1 Place the Spin Column in the Collection Tube, add 250 μl Buffer BL, and centrifuge at 12,000 rpm / min for 1 min to activate the silica membrane;
[0041] 1.2 Take the sample and dry the tissue (not more than 20 mg), add liquid nitrogen and grind it thoroughly. After grinding, put it into a 1.5 ml centrifuge tube, add 400 μl Buffer gP1, vortex for 1 min, and in a water bath at 65 °C for 10 to 30 min, during which time it can be taken out and mixed to fully lyse;
[0042] 1.3 Add 150 μl Buffer gP2, vortex for 1 min, and ice bath for 5 min;
[0043] 1.4 Centrifuge at 12,000 rpm / min for 5 min, and transfer the supernatant to a new centrifuge tube;
[0044] 1.5 Add an equal volume of anhydrous ethanol to the supernatant, immediately shake and mix thoroughly, transfer all the liquid to the Spin Column,...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention belongs to the field of sweet potato breeding, and particularly relates to a sweet potato anthocyanin related SNP molecular marker, identification and application thereof. The sweet potato anthocyanin related SNP molecular marker disclosed by the invention is an SNP site (chr12.20497784), and the genotypes of the sweet potato anthocyanin related SNP molecular marker are GG, GA and AA. Compared with the conventional population strain total resequencing analysis SNPs, the identification method provided by the invention has the advantages of small data volume, low cost and the like. According to the method provided by the invention, the leaves are taken in a seed stage or a seedling stage, and the SNP sites can be subjected to genetic typing through an SNaPshot technical platform to verify whether the plant potato tubers contain the anthocyanin, so that compared with the conventional method that whether the plant potato tubers contain the anthocyanin can be known after tuberization, the method has the advantage that the breeding time is saved.
Description
technical field [0001] The invention belongs to the field of sweet potato breeding, in particular to a sweet potato anthocyanin-related SNP molecular marker, identification and application thereof. Background technique [0002] Anthocyanin is a natural water-soluble food coloring. sweet potato( Ipomoea batatas (L.) Lam ) accumulation of anthocyanins in germplasm resources showed abundant polymorphisms. Anthocyanins in sweet potatoes are widely used in food, medicine and other fields. As the latest generation of genetic molecular markers, single nucleotide polymorphisms (SNPs) are widely used in various biological research fields. At present, there is no systematic correlation analysis among the anthocyanin accumulation traits in each growth site of sweet potato. Breeding purple sweet potato varieties requires that the seeds obtained by sexual hybridization are first seeded in the seedbed, and then transplanted into the field. After the potatoes are set, it is possible to...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
Inventor 秦桢王庆美侯夫云李爱贤董顺旭周媛媛张海燕解备涛段文学张立明
Owner CROP RES INST SHANDONG ACAD OF AGRI SCI