Primer group for detecting IHHNV, EHP and WSSV, kit and application thereof

Abstract

The invention discloses a primer group for detecting IHHNV, EHP and WSSV, a kit and application thereof, and relates to the technical field of molecular biology. The primer group for detecting the IHHNV, the EHP and the WSSV disclosed by the invention comprises the following components: IHHNV-F and IHHNV-R, EHP-F and EHP-R, and WSSV-F and WSSV-R. The primer group is strong in specificity and high in sensitivity, and can be used for detecting IHHNV, EHP and WSSV. A method based on the group of primers has the characteristics of simplicity in operation, high sensitivity, strong specificity, high detection flux, high detection speed and low cost, a result is obtained through direct analysis of fluorescence data, and a result interpretation mode is simple and direct.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a set of primers, kits and applications for detecting IHHNV, EHP and WSSV. Background technique [0002] In recent years, the scale of prawn farming has continued to expand, and the prevalence and spread of virus infection have become increasingly serious, showing a trend of diversification of infectious pathogens. Infectious hypodermal and hematopoietic necrosis virus (IHHNV), enterocytozoon hepatopenaei (EHP) and whitespot syndrome virus (WSSV) are three major pathogens that seriously damage prawns. Seriously affect the sustainable development of the shrimp farming industry, so strict quarantine must be carried out to the farming production process. [0003] Infectious hypodermic and hematopoietic necrosis virus (IHHNV) belongs to the family Parvoviridae, the subfamily Densoviridae, and the genus Densovirus, and is the smallest virus among known shrimp viruses. IHHN...

AI Technical Summary

Problems solved by technology

Microspore inspection is a commonly used detection and diagnosis method for microspores in production practice, but some microspore spores are so small that it is difficult to observe them under a microscope

Ordinary PCR method is a traditional method for the diagnosis of many shrimp pathogens. Although the results are accurate, the sensitivity is not enough and the operation process is time-consuming, which limits the popularization and application of ordinary PCR detection methods in production to a certain extent; ring-mediated reverse transcription isothermal amplification Although the detection time is short and the operation is simple, the false positive rate is high, which has been criticized by professionals; the fluorescent PCR method and CRISPR technology are not only fast, simple and practical, but also have high detection sensitivity, and can also detect latent infectious materials and early infectious materials , but in the actual sample detection work, these two methods still have the problems of low throughput, relying on professional human operation, low efficiency when dealing with large-scale detection requirements, and high detection cost

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