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Biomarker combination for white matter lesion and application of biomarker combination

A biomarker, white matter technology, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of high cost, missed treatment time, expensive testing equipment, etc., and achieve the effect of accurate test results and cost reduction.

Active Publication Date: 2022-04-01
BAO FENG BIOTECH (BEIJING) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection equipment used is expensive and costs a lot
Generally, CT and MRI will be performed for further diagnosis unless severe symptoms appear, which will miss the best time for treatment

Method used

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  • Biomarker combination for white matter lesion and application of biomarker combination
  • Biomarker combination for white matter lesion and application of biomarker combination
  • Biomarker combination for white matter lesion and application of biomarker combination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) The control group used for model establishment was 29 people: there was no abnormality in the MRI nuclear magnetic resonance examination, and the age range was above 45.

[0043] A group of 49 patients: MRI nuclear magnetic resonance showed infarcts in the white matter of the brain, and the age range was over 45. Serum samples were collected from the above-mentioned people and corresponding clinical data were collected.

[0044] Take 200 μL of the collected serum samples and extract with 600 μL of pre-cooled isopropanol, vortex with a vortex shaker for 1 min, incubate at room temperature for 10 min, then store the extraction mixture at -20 °C overnight, centrifuge at 4000rr in a refrigerated centrifuge for 20 min, Transfer the supernatant to a new centrifuge tube and dilute to 1:10 with a 2:1:1 volume ratio of isopropanol:acetonitrile:water mixture. Samples were stored at -80°C until LC-MS analysis. Additionally, pooled serum samples were prepared by combining 10 µ...

Embodiment 2

[0078] The above-mentioned logistic regression model was verified using an external data set, and the sampling standard was the same as that in Example 1. The sample group for model verification was 130 people, 85 people were patients, and 45 people were normal controls. Using the method in Example 1 to carry out ultra-high performance liquid chromatography-mass spectrometry method for serum ceramide (d18:0 / 24:1 (15Z)), phosphatidylcholine (O-18:0 / 0:0) and galactosylceramide (d18:0 / 16:0) were detected, combined with clinical indicators including weight (kg), height (cm), systolic blood pressure (mmHg), diastolic blood pressure (mmHg), Any two indicators in triglyceride, high-density lipoprotein, low-density lipoprotein, fasting blood glucose, homocysteine, and glycosylated hemoglobin, to verify the accuracy of the model results in Example 1, and draw the corresponding ROC curve Figure, the result is as follows:

[0079] "Model A" variable is R1+R28+R30+K4+K9, the result of th...

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Abstract

The invention provides a biomarker combination for white matter lesion and application thereof, and particularly relates to application of biomarkers, namely ceramide (d18: 0 / 24: 1 (15Z)), phosphatidylcholine (O-18: 0 / 0: 0) and galactosylceramide (d18: 0 / 16: 0), in preparation of a detection reagent for diagnosing white matter lesion. The risk of white matter lesion is judged through biomarkers ceramide (d18: 0 / 24: 1 (15Z)), phosphatidylcholine (O-18: 0 / 0: 0) and galactosylceramide (d18: 0 / 16: 0) in combination with any two of clinical indexes including weight, height, systolic pressure, diastolic pressure, triglyceride, high-density lipoprotein, low-density lipoprotein, fasting blood-glucose, homocysteine or glycosylated hemoglobin, and the detection result is accurate and reliable. In combination with a detection model, white matter lesion can be prevented in advance.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a combination of biomarkers for cerebral white matter lesions and an application thereof. Background technique [0002] White matter lesions mainly cause symptoms such as cognitive impairment, speech impairment, abnormal mental behavior, gait disorder, and urination disorder, which seriously affect the quality of life of patients. It is strongly associated with an increased risk of stroke and dementia. With the continuous development of imaging technology, the detection rate of white matter lesions is getting higher and higher. Factors such as hypertension, diabetes, dyslipidemia, and metabolic disorders are also closely related to the onset and progression of white matter lesions, and these metabolic diseases mainly occur in the elderly population. In general, the condition of most patients with white matter lesions is reversible, so taking appropriate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72
Inventor 陈显扬宋王婷常婷婷
Owner BAO FENG BIOTECH (BEIJING) CO LTD