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Modified GPR75 and application thereof

A sequence and amino acid technology, applied in the field of modified GPR75 and its application, to achieve high expression, good stability, and the effect of improving stability and expression

Active Publication Date: 2022-04-05
SHUIMU BIOSCIENCES LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is very challenging to obtain the structure of the inactive receptor and develop specific inhibitors of receptor activity.

Method used

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  • Modified GPR75 and application thereof
  • Modified GPR75 and application thereof
  • Modified GPR75 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0073] Embodiment: the preparation of transformation type GPR75

[0074] 1. Sequence optimization

[0075] In this example, the protein structure prediction software used is AlphaFold local version (v2.1.0) and RoseTTAFold.

[0076] 1.1. Truncation and transformation of wild-type GPR75

[0077] Wild-type GPR75 has a long random sequence region, which is not suitable for protein structure analysis. In this example, according to AlphaFold2 and RoseTTAFold two models for GPR75 secondary structure prediction ( Figure 1A ), the predicted results for the transmembrane region are very similar. The reliability of the random sequence region is very low, so we truncated the original random sequence region of the GPR75 receptor, leaving the key seven transmembrane regions of the receptor ( Figure 1B ). The wild-type and its truncated sequences are shown below (see SEQ ID NO: 1 for details), truncated between the fifth transmembrane helix and the sixth transmembrane helix of wild...

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PUM

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Abstract

The invention discloses a modified GPR75 (Glutathione Protein Receptor 75) and application thereof. The present invention provides a modified GPR75 comprising: a first domain comprising an amino acid sequence derived from a [beta] 2 adrenergic receptor, a second domain comprising an amino acid sequence derived from a [beta] 2 adrenergic receptor, and a third domain comprising an amino acid sequence derived from a [beta] 2 adrenergic receptor, and the second structural domain is a structural domain which is formed by deleting an irregular sequence between a fifth transmembrane helix and a sixth transmembrane helix and irregular sequences of an N terminal and a C terminal in wild type GPR75 and is connected between the fifth transmembrane helix and the sixth transmembrane helix through an amino acid sequence derived from BRIL fusion protein. The modified GPR75 provided by the invention can be used for GPR75 structure analysis, fluorescent molecular marking, phosphorylated polypeptide or signal protein fusion, GPR75 activity analysis, nucleic acid coding small molecule library screening, and computer-aided drug design and drug screening.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically, the invention relates to a modified GPR75 and its application. Background technique [0002] G protein-coupled receptors are the largest class of cell membrane receptors in the human body. The completion of the Human Genome Project provides a basis for analyzing the distribution, sequence and function of the family members 1 . There are more than 800 G protein-coupled receptor members in the human body, including about 370 non-olfactory G protein-coupled receptors and more than 400 olfactory receptors. These G protein-coupled receptors are divided into six subfamilies, the rhodopsin family, the adhesion family (secretin family), the secretin receptor family (secretin family), the glutamate receptor family (glutamate family), Frizzled family, and Tasted family. Different G protein-coupled receptors are involved in mediating a series of important biological functions of organ...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/62
CPCC12N15/62C07K19/00C07K14/72
Inventor 衡杰郭涵博杨怡然何鋆彤李京卓微倪晓丹
Owner SHUIMU BIOSCIENCES LTD
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