Breeding method and application of deep sea salt-tolerant phenol degrading bacteria

Abstract

The invention discloses a breeding method and application of deep-sea salt-tolerant phenol degrading bacteria. The method comprises the following steps: (1) enriching deep-sea salt-tolerant bacteria; (1.1) preparing a culture medium; (1.2) enriching the mixed bacterial liquid: taking a deep sea sediment sample, adding the deep sea sediment sample into a culture medium, culturing for 5 days, then transferring the deep sea sediment sample into a fresh culture medium which is treated in the same way according to the inoculum size (v / v) of 10%, and continuously enriching and culturing for four times; detecting the concentration of strains in the fifth-generation enriched liquid; when the bacterium concentration of the fifth-generation culture solution is greater than 1 * 10 < 9 > / mL after the fifth-generation culture solution is cultured for 5 days, the enrichment culture is finished, and an enrichment culture bacterium solution is obtained; and (2) domesticating the salt tolerance of the mixed strain: carrying out domestication culture on the enrichment culture bacterial liquid, and carrying out gradient domestication on the salt tolerance of the enrichment culture bacterial liquid by adopting a method for gradually increasing the concentration of NaCl in a culture medium for domestication culture. The problem that phenol is difficult to degrade by microorganisms in a high-salt environment can be solved.

Description

technical field [0001] The invention relates to the technical field of microbial degradation, in particular to a breeding method and application of deep-sea salt-tolerant phenol-degrading bacteria. Background technique [0002] Phenol is a common phenolic substance, widely used in the production process of fungicides, resins, petroleum and pharmaceuticals, etc. The discharge of phenolic wastewater not only causes serious pollution to the environment, but also endangers the health of animals, plants and humans . Long-term drinking of water or food contaminated by phenol will damage the liver and kidney function, and even lead to death. Therefore, this type of wastewater has the characteristics of wide sources, large amount of water, great harm and high salinity. [0003] As a pollutant that needs to be controlled preferentially, the treatment methods of phenolic wastewater are mainly divided into physical, chemical and biological methods. Among them, the biological method ...

AI Technical Summary

Problems solved by technology

[0005] The problem to be solved by the present invention is to provide a breeding method and application of deep-sea salt-tolerant phenol-degrading bacteria, which can solve the problem that microorganisms are difficult to degrade phenol in a high-salt environment

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