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Oil-degrading composite bacterium and microbial inoculum applied to high salt environment

A technology of petroleum degradation and compound bacterial agent, applied in the field of environmental restoration, can solve the problems of difficult biodegradation of petroleum pollutants, and achieve the effect of fast removal speed, high activity and strong adaptability

Active Publication Date: 2014-12-03
CHINA NAT OFFSHORE OIL CORP +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the deficiencies of the prior art, aiming at the problem that petroleum pollutants are difficult to biodegrade in the current high-salt environment, to provide a kind of petroleum-degrading composite bacteria applied in the high-salt environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Connect Ornithinimicrobium kibberense W25 and Gordonia alkanivorans W33 strains to beef extract peptone medium (beef extract 5g, peptone 10g, NaCl5g, water 1000ml, pH 7.0 (can also be 7.1 or 7.2), and sterilize at 121°C for 20 minutes) , 32 ℃ constant temperature shaker 120r / min culture for 72 hours. Centrifuge at 6000r / min for 5 minutes, remove the lower layer of bacterial pellet, and wash it twice with phosphate buffer at pH 7.0 (it can also be 7.1, 7.2, 7.3, 7.4 or 7.5). The obtained bacterial pellets were mixed according to the formula in Table 1 to obtain a petroleum-degrading composite bacteria and a bacterial agent for the composite bacteria applied in a high-salt environment.

[0044] Table 1. Composition and ratio of formula (each component is part by weight)

[0045]

Embodiment 2

[0047] Experimental group: 5g of a petroleum-degrading compound microbial agent used in high-salt environments prepared with formula 6 was mixed with 200g of petroleum-polluted saline soil;

[0048] Control group: Take 200g of the same petroleum-polluted saline soil as the control group;

[0049] The above-mentioned oil-polluted salinized soil has a salt concentration of 0.3% (after testing, it contains NaCl 0.22% Na 2 SO 4 0.04%); standing in a dark place at room temperature for 20 days, the total petroleum hydrocarbon content of the experimental group decreased from 20.3mg / g to 9.3mg / g, the degradation rate of petroleum hydrocarbon reached 54.2%, and the degradation rate of petroleum hydrocarbon in the control group was 12.9%.

Embodiment 3

[0051] Experimental group: 10g of a petroleum-degrading compound microbial agent used in high-salt environments prepared with formula 7 was mixed evenly with 200g of petroleum-polluted saline soil;

[0052] Control group: Take 200g of the same petroleum-polluted saline soil as the control group;

[0053] The above-mentioned oil-polluted saline soil has a salt concentration of 0.5% (after testing, it contains 0.34% NaCl 2 SO 4 0.11%); standing in a dark place at room temperature for 20 days, the total petroleum hydrocarbon content of the experimental group decreased from 20.3mg / g to 7.2mg / g, the degradation rate of petroleum hydrocarbon reached 64.5%, and the degradation rate of petroleum hydrocarbon in the control group was 15.7%.

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Abstract

The invention discloses an oil-degrading composite bacterium and microbial inoculum applied to a high salt environment. The composite bacterium is composed of a bacterium W25 and a bacterium W33, wherein the bacterium W25 is named Ornithinimicrobium kibberense of which the microbial collection number is CGMCC No.6844; the bacterium W33 is named Gordonia alkanivorans of which the microbial collection number is CGMCC No.6845; and the oil-degrading composite bacterium has the capacity for degrading oil in a high salt environment. The experiment proves that the composite bacterium and microbial inoculum disclosed by the invention have high speed for clearing oil pollution in a high salt environment; the composite bacterium and microbial inoculum disclosed by the invention have wide adaptability to salinity of the oil pollution environment, and can be applied to the high salt environment with the maximum salinity of 8% NaCl; and the composite bacterium and microbial inoculum disclosed by the invention can be used for quickly and effectively treating oil pollution salinized soil, oil pollution seawater, oil pollution wastewater and the like.

Description

Technical field [0001] The invention belongs to the technical field of environmental remediation, and relates to a petroleum degrading bacterial agent applied in a high-salt environment and a preparation method. Background technique [0002] With the continuous enhancement of human oil exploration and development activities, the environment is also bearing a huge risk of oil pollution. A large amount of petroleum pollutants enter the environment through accidents and other means, causing petroleum pollution. Petroleum pollution often occurs in high-salt environments, resulting in oil-polluted high-salt environments, such as oil pollution of salinized soil, oil pollution of high-salt wastewater, and oil pollution of seawater. Due to the special geographical location of many large oil fields in my country, the soil is salinized to varying degrees. Accidental leakage in the process of oil extraction is very easy to cause oil pollution and form oil-polluted saline soil. In additio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/14C12N11/10B09C1/10C02F3/34C12R1/01C02F101/32
Inventor 王新新吴亮杨勇安伟牛志刚丁东华
Owner CHINA NAT OFFSHORE OIL CORP
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