A kind of low-temperature nitrifying pseudomonas and its application

A Pseudomonas, low-temperature technology, applied in the field of environmental microorganisms, can solve the problem of the effluent quality of sewage treatment plants being difficult to meet the standard, saving domestication time and facility construction costs, high sewage treatment efficiency, and wide range of salinity adaptation. Effect

Active Publication Date: 2020-01-07
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a strain of low-temperature Pseudomonas nitrificans screened from Arctic marine sediments to solve the problem that the effluent water quality of sewage treatment plants in northern my country is difficult to meet the standard due to low temperature

Method used

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  • A kind of low-temperature nitrifying pseudomonas and its application
  • A kind of low-temperature nitrifying pseudomonas and its application
  • A kind of low-temperature nitrifying pseudomonas and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The low-temperature Pseudomonas bacteria involved in this example were obtained by screening methods such as enrichment, dilution, and selective medium cultivation of Arctic marine sediments.

[0022] 1. Materials used:

[0023] From July to September 2014, marine sediments collected during China's sixth Arctic scientific expedition.

[0024] 2. Medium composition:

[0025] Heterotrophic nitrification medium (g / L): (NH 4 ) 2 SO 4 1, Sodium acetate 2.5, Sodium citrate 2.5, K 2 HPO 4 5. MgSO 4 2.5, NaCl 20, trace element solution 2mL.

[0026] Wherein said trace element solution (g / L): ZnSO 4 2.2, CaCl 2 5.5, MnCl 2 5.06, FeSO 4 5.0, ammonium molybdate 1, CuSO 4 1.57, CoCl 2 1.61. Seawater Zobell 2216E medium (g / L): peptone 5, yeast powder 1, filtered aged seawater: tap water (v / v2:1).

[0027] 3. Screening method:

[0028] Take an appropriate amount of arctic marine sediment samples, transfer them into 50mL liquid heterotrophic nitrification mediu...

Embodiment 2

[0043] Detection of Nitrification Activity of Strain CC6-YY-74

[0044] (1) Inoculate the purified bacterial strain CC6-YY-74 with a 4% inoculum in 100 mL of sterilized nitration medium with a NaCl concentration of 30‰, and cultivate them at 0°C, 10°C, 20°C and 30°C Chamber shaking culture, the rotating speed is 130r / min. Ammonia nitrogen (NH 4 -N) changes in concentration, the results are as figure 2 shown.

[0045] (2) The purified bacterial strain CC6-YY-74 was inoculated in 100mL sterilized nitrification medium with different concentrations of NaCl (NaCl concentrations were 0‰, 15‰, 30‰, 45‰, 60‰, respectively) with an inoculum size of 4%. ‰ and 90‰), cultivated at 10°C and 130r / min shaking, and detected the ammonia nitrogen (NH 4 -N) changes in concentration, the results are as image 3 shown.

[0046] Composition of the above nitrifying medium (g / L): NH 4 Cl 0.191, KH 2 PO 4 0.05, MgSO 4 0.1, sodium acetate 2.5, sodium citrate 2.5, trace element solution 2mL...

Embodiment 3

[0049] Detection of denitrification activity of strain CC6-YY-74

[0050] (1) Inoculate the purified strain CC6-YY-74 with a 4% inoculum in 100 mL of sterilized denitrification medium with a NaCl concentration of 30‰, and cultivate them at 0°C, 10°C, 20°C and 30°C Chamber shaking culture, the rotating speed is 130r / min. The nitrate nitrogen (NO) in the medium was detected every 24 h 3 -N), nitrite nitrogen (NO 2 -N) changes in concentration, the results are as Figure 4 shown.

[0051] (2) The purified bacterial strain CC6-YY-74 was inoculated in 100 mL of sterilized denitrification medium with different concentrations of NaCl (NaCl concentrations were 0‰, 15‰, 30‰, 45‰, 60‰ and 90‰), cultivated at 10°C and 130r / min shaking, and detected nitrate nitrogen (NO 3 -N), nitrite nitrogen (NO 2 -N) changes in concentration, the results are as Figure 5 shown.

[0052] The above denitrification medium composition (g / L): KNO 3 0.144, KH 2 PO 4 0.05, MgSO 4 0.1, sodium ac...

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Abstract

The invention discloses a low-temperature nitrifying pseudomonas and its application, which is obtained by screening from Arctic marine sediments and is named as pseudomonas Pseudomonas sp. CC6‑YY‑74, deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms, with the preservation number CGMCC No. 12611, and the preservation date is June 15, 2016. The Pseudomonas has strong nitrification and denitrification activities at low temperatures, and has a wide range of salinity adaptability. Its use in sewage treatment can solve the problem that the effluent water quality of sewage treatment plants in northern my country is difficult to meet the standard due to low temperature. The problem.

Description

technical field [0001] The invention belongs to the technical field of environmental microbes, and in particular relates to a strain of nitrifying-arctic low-temperature pseudomonas, a screening method of the bacterium, and an application in nitrogen-containing wastewater treatment. Background technique [0002] With the continuous improvement of urbanization in our country, the control of small and medium-sized point source pollution is the key to improving the quality of water environment and solving the problem of water shortage. The winter in the cold regions of our country is long, and the biological treatment of domestic sewage has always had problems such as low microbial activity, poor biochemical treatment effect, and difficulty in reaching water standards. Performance, microbial growth and development, population composition and oxygen transfer efficiency in the aeration tank all have a significant impact. [0003] At present, in order to alleviate the impact of l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/16
CPCC02F3/34C02F2101/16C02F2101/163C02F2101/166C12N1/205C12R2001/38
Inventor 林学政李晓亮何培青张培玉
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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