A strain of sea bacterium with aerobic denitrification ability and its application

An aerobic denitrification and sea bacillus technology, applied in bacteria, chemical instruments and methods, microorganism-based methods, etc., can solve the problem of less salt-tolerant aerobic denitrification bacteria, achieve easy collection and fixation, and increase practical performance Effect

Active Publication Date: 2020-07-10
THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many reports on aerobic denitrification bacteria, but there are relatively few salt-tolerant aerobic denitrification bacteria from marine sources, which use nitrogen as the end product and do not release N. 2 O has fewer aerobic denitrifying bacteria, so isolation and screening from the marine environment has a wide range of salinity adaptation, high nitrogen removal efficiency, and low or no N production. 2 O microbial strains, the development of microbial preparations derived from marine strains is an effective strategy for purifying aquaculture water, denitrification of high-salt sewage, and remediation of marine nitrogen pollution

Method used

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  • A strain of sea bacterium with aerobic denitrification ability and its application
  • A strain of sea bacterium with aerobic denitrification ability and its application
  • A strain of sea bacterium with aerobic denitrification ability and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Example 1 Isolation and Identification of Marinobacter sp. 1A14215

[0040] 1. Isolation of bacterial strains: take the sediment overlying water sample of 4432m in the Southwest Indian Ocean (E50 ° 45.06' S33 ° 16.74'), adopt the gradient dilution coating plate method, and use the Marine Agar solid medium to coat the plate for separation and purification to obtain Marinobacter (Marinobacter sp.) 1A14215 single bacteria.

[0041] 2. Physiological and biochemical characteristics and molecular identification of the strain

[0042] Morphological characteristics: Marinobacter sp. 1A14215, Gram-negative, cultured on Marine Agar solid medium at 28°C for 48 hours, the colonies are beige, smooth, slightly raised, with regular, opaque edges, and the diameter of the colonies is about It is 1~2mm. Individual strains are rod-shaped, without spores, and have flagella.

[0043] Physiological and biochemical characteristics: Marinobacter sp. 1A14215 grows at a salinity of 0-12%, and...

Embodiment 2

[0047] Example 2 Marinobacter sp. 1A14215 draft genome sequencing and denitrification gene analysis

[0048] Genomic DNA of Marinobacter sp. 1A14215 was extracted according to conventional methods, and sent to Shanghai Meiji Gene Co., Ltd., using the Hiseq platform for genome draft sequencing, and quality control, assembly and annotation of the obtained sequences. After annotation, it was found that the genome of Marinobacter sp. 1A14215 has a complete denitrification metabolic pathway: including nitrate reductase gene (nar), nitrite reductase gene (nir), nitric oxide reductase gene (nor) , nitrous oxide reductase gene (nos), and other denitrification-related transport regulatory genes.

Embodiment 3

[0049] Example 3 Optimization of Nitrogen Removal Conditions for Marinobacter sp. 1A14215

[0050] Pick a single colony of Marinobacter sp. 1A14215 and inoculate it into 100 mL of Marine Broth2216 (purchased from BD Difco) liquid medium. After culturing at 150 rpm at 28°C for 24 hours, take 30 mL of centrifuge to collect the bacteria, resuspend in 0.8% saline, Centrifuge, wash repeatedly 2 to 3 times, and finally make a bacterial suspension with normal saline, and adjust the concentration of the bacterial solution to 2×10 8 cfu / mL, inoculate with 0.2% inoculum to contain 140mg / L NO 3 - In the basal medium of -N, the denitrification conditions of the strains were measured under different carbon sources, C / N ratios, pH values, salinity, and temperature conditions, and the denitrification conditions were optimized.

[0051] Basal medium: KH 2 PO 4 0.5g / L, Na 2 HPO 4 0.5g / L, MgSO 4 ·7H 2 O 0.05g / L, FeSO 4 ·7H 2 O0.02g / L, CaCl 2 0.02g / L, distilled water 1000mL, steriliz...

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Abstract

The invention discloses a Marinobacter strain with aerobic denitrification capability and application thereof, and relates to the Marinobacter. The Marinobacter sp.1A14215 is separated from deep sea water with the depth of 4432m in South Indian Ocean. The Marinobacter sp. 1A14215 can perform denitrification nitrogen removal by taking nitrate and nitrite as unique nitrogen sources respectively under aerobic and anaerobic conditions, and a denitrification end product is N2 and N2O is not released. The denitrification action is carried out in the nitrogen-containing simulated wastewater, and thecarbon sources of the nitrogen-containing simulated wastewater are respectively sodium citrate, sodium acetate, sodium lactate, sodium succinate, sodium propionate, glycerol and maltose. The Marinobacter sp.1A14215 can be applied to biological denitrification of the nitrogen-containing wastewater and sea freshwater aquaculture.

Description

technical field [0001] The invention relates to sea bacterium, in particular to a strain of sea bacterium with aerobic denitrification ability and application thereof. Background technique [0002] In natural water bodies, nitrogen exists in nine different valence forms from -3 to +5. Under the joint action of biotic and abiotic factors, they are continuously transferred and transformed in the water body, forming a complex dynamic cycle. With the development of modern industry and agriculture, the problem of nitrogen pollution to the environment has become increasingly serious. Water eutrophication caused by ammonia nitrogen, nitrate and nitrite polluted water bodies is becoming more and more serious, which not only causes great damage to freshwater and marine ecosystems, but also leads to the pollution of groundwater and drinking water. At the same time, under the intensive management and high-density farming of modern commercial farming models, the accumulation of nitroge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16C02F103/20
Inventor 邵宗泽王丽萍王蕾李建洋
Owner THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
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