Method and system for improving repair capability of mesenchymal stem cells

A technology of mesenchymal stem cells and bone marrow mesenchyme, applied in the field of improving the repair ability of mesenchymal stem cells, can solve the problems of low repair ability of mesenchymal stem cells, achieve the effect of preventing calcium transport obstacles, effectively tracking, and improving repair ability

Pending Publication Date: 2022-04-05
EASTERN UNION STEM CELL & GENE ENG
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] In view of the problems existing in the above-mentioned prior art, the present invention proposes a method and system for improving the repair

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  • Method and system for improving repair capability of mesenchymal stem cells
  • Method and system for improving repair capability of mesenchymal stem cells

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Embodiment Construction

[0040] The following is a detailed description of the embodiments of the present invention. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following implementation example.

[0041] The terms "first", "second", "third", "fourth", etc. (if any) in the description and claims of the present invention and the above drawings are used to distinguish similar objects and not necessarily Describe a specific order or sequence. It is to be understood that the data so used are interchangeable under appropriate circumstances such that the embodiments of the invention described herein can be practiced in sequences other than those illustrated or described herein. Furthermore, the terms "comprising" and "having", as well as any variations thereof, are intended to cover a non-exclusive in...

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Abstract

The invention discloses a method and a system for improving the repair capability of mesenchymal stem cells. The method comprises the following steps: obtaining mesenchymal stem cells with stable subculture; culturing the mesenchymal stem cells for 24-48 hours under the conditions of ischemia and hypoxia; transfecting the mesenchymal stem cells subjected to hypoxia and ischemia culture by using calcium binding protein; and culturing the transfected mesenchymal stem cells under a normal oxygen condition for a preset time for later use. The system comprises a mesenchymal stem cell obtaining module, an ischemia and hypoxia culture module, a transfection module and a normal oxygen culture module. According to the invention, through hypoxia-ischemia culture and calcium binding protein transfection, the repair capability of the mesenchymal stem cells after transplantation is improved.

Description

technical field [0001] The invention relates to the technical field of mesenchymal stem cells, in particular to a method and system for improving the repair ability of mesenchymal stem cells. Background technique [0002] The ability of mesenchymal stem cells to self-renew and differentiate into tissue-specific cells gives them great potential in the field of regenerative medicine. Mesenchymal stem cells coordinate the development, maintenance and repair of tissues, and produce a variety of secreted factors, which can play a significant therapeutic role in bone defects, myocardial infarction, nervous system damage and other diseases. [0003] Current studies have found that mesenchymal stem cells are cultured and differentiated into myocardial precursor cells in vitro using a unique cell recombination technology, and implanted into the damaged heart through a cardiac catheter, which can achieve cardiac muscle, but it cannot solve the abnormal molecular mechanism of heart fai...

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Application Information

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IPC IPC(8): C12N5/0775C12M1/36C12M3/00
Inventor 陆梦晓李建有刘乐锋刘洋
Owner EASTERN UNION STEM CELL & GENE ENG
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