Sterilizing agent and sterilizing method using sterilizing agent

A sterilizing method and a sterilizing agent technology, which are applied in the field of sterilizing agents and sterilizing agents, can solve problems affecting operations, etc., and achieve reduced collagen structure damage, good sterilizing effect, and good sterilizing effect Effect

Active Publication Date: 2022-04-12
SUZHOU HAOBEN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the inventors found that in the steps before decellularization such as removal of remaining tissues, the growth of bacteria and the bacteria brought by external operations will increase the bacteria in biological tissues, which will affect the subsequent operations

Method used

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  • Sterilizing agent and sterilizing method using sterilizing agent
  • Sterilizing agent and sterilizing method using sterilizing agent
  • Sterilizing agent and sterilizing method using sterilizing agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 provides a kind of sterilizing agent, and sterilizing agent comprises peracetic acid, hydrogen peroxide and solvent; In sterilizing agent, the concentration of peracetic acid is 1.563ml / L, and the concentration of hydrogen peroxide is 7.813ml / L, The solvent is saline.

[0042] The configuration method of a kind of sterilant is:

[0043] Get the aqueous solution of peracetic acid and hydrogen peroxide as stock solution, wherein the concentration of peracetic acid is 50ml / L, and the concentration of hydrogen peroxide is 250ml / L. The stock solution was diluted step by step with physiological saline, and the number of dilutions in this embodiment was 5 times, and the dilution factor was 32 times.

[0044] Primary dilution: Take 25ml of the stock solution and mix with 25ml of normal saline to obtain a primary mixture.

[0045] Secondary dilution: Take 25ml of the primary mixture and mix with 25ml of normal saline to obtain the secondary mixture.

[0046] Tert...

Embodiment 2-6

[0053] The difference between Examples 2-6 and Example 1 is that the temperature and time in the sterilization process are different, see Table 1 for details.

[0054] The sterilization condition parameter table of table 1 embodiment 1-6

[0055]

Embodiment 7

[0057] The difference between the sterilizing agent in Example 7 and the sterilizing agent in Example 1 is: the stock solution is diluted 6 times, and the dilution factor is 64 times to obtain the sterilizing agent. In the sterilizing agent, the concentration of peracetic acid is 0.781ml / L, and the concentration of hydrogen peroxide is 3.906ml / L.

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PUM

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Abstract

The invention relates to the field of decellularized tissue treatment, and particularly discloses a sterilizing agent and a sterilizing method applying the sterilizing agent, the sterilizing agent comprises peracetic acid and hydrogen peroxide, and the volume dosage ratio of the peracetic acid to the hydrogen peroxide is 1: (0.26-84.5); the sterilizing agent is used for sterilizing biological tissues in a decellularization treatment process. The sterilization method comprises the following steps: taking a biological tissue; the biological tissue is put into a sterilizing agent to be sterilized; the sterilization temperature is 3-50 DEG C. According to the present invention, the biological tissue can be well sterilized before the decellularization treatment.

Description

technical field [0001] The present application relates to the field of decellularized tissue processing, more specifically, it relates to a sterilizing agent and a sterilizing method using the sterilizing agent. Background technique [0002] Decellularized tissue is a material derived from a living body. Human or animal tissue with the same structure as human tissue is processed to remove the immunogenic components in the tissue to obtain acellular tissue. Acellular materials are often used in the fields of scaffold materials for regenerative medicine and materials for promoting wound repair, and acellular materials have attracted much attention due to their high efficiency. [0003] When producing decellularized tissue for research or clinical use, animal tissue is taken as a raw material in a clean environment, and then the animal tissue is decellularized. However, even animal tissues collected in a clean environment can carry bacteria and viruses themselves. Therefore, i...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): A01N59/00A01N37/02A01P1/00A61L2/00A61L101/22A61L101/36
CPCA61L2/00A01N37/02A01P1/00A01N59/00A61L2101/00
Inventor船本诚一张永巍
OwnerSUZHOU HAOBEN BIOTECHNOLOGY CO LTD