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Multi-channel conductive nerve graft and preparation method thereof

A nerve transplantation and multi-channel technology, applied in the field of multi-channel conductive nerve grafts and their preparation, can solve the problems of inability to satisfy the conductivity and orientation at the same time, poor degradation performance, etc., and achieve the effects of accelerating regeneration, promoting growth and differentiation

Active Publication Date: 2022-04-12
TIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The electrical conductivity and orientation of the nerve grafts disclosed in the prior art cannot be satisfied at the same time, and have disadvantages such as poor degradation performance

Method used

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  • Multi-channel conductive nerve graft and preparation method thereof
  • Multi-channel conductive nerve graft and preparation method thereof
  • Multi-channel conductive nerve graft and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A method for preparing a multi-channel conductive nerve graft of the present embodiment, the method comprises the following steps:

[0039] (1) Concentrate the freshly extracted silk fibroin solution at 50°C to a concentration of 15%, then dilute to a silk fibroin concentration of 3%, and place it at 50°C for 28 hours;

[0040] (2) Pour the placed silk fibroin solution into a plastic tube, and place the plastic tube in a one-way freezer for freezing treatment. The bottom of the plastic tube is in direct contact with the copper plate. The refrigerant is a mixture of acetone and dry ice. The temperature of the refrigerant is The temperature is -80°C, the freezing time is 20min, and then the plastic tube is moved to the freeze dryer for 20h to obtain the scaffold;

[0041] (3) Cut the stent into a cylinder with a diameter of 2 mm and a length of 10 mm, treat it with methanol for 25 minutes to make it no longer soluble in water, and freeze-dry;

[0042] (4) Soak the freeze...

Embodiment 2

[0044] A method for preparing a multi-channel conductive nerve graft of the present embodiment, the method comprises the following steps:

[0045] (1) Concentrate the freshly extracted silk fibroin solution at 60°C to a concentration of 20%, then dilute to a silk fibroin concentration of 4%, and place it at 60°C for 24 hours;

[0046] (2) Pour the placed silk fibroin solution into a plastic tube, and place the plastic tube in a one-way freezer for freezing treatment. The bottom of the plastic tube is in direct contact with the copper plate. The refrigerant is a mixture of acetone and dry ice. The temperature of the refrigerant is The temperature is -75°C, the freezing time is 40 minutes, and then the plastic tube is moved to a freeze dryer for 24 hours to obtain a scaffold;

[0047] (3) Cut the stent into a cylinder with a diameter of 6 mm and a length of 6 mm, treat it with methanol for 30 minutes to make it no longer soluble in water, and freeze-dry;

[0048](4) Soak the fr...

Embodiment 3

[0050] A method for preparing a multi-channel conductive nerve graft of the present embodiment, the method comprises the following steps:

[0051] (1) Concentrate the freshly extracted silk fibroin solution in a 70°C environment to a concentration of 25%, then dilute to a silk fibroin concentration of 5%, and place it in a 70°C environment for 20 hours;

[0052] (2) Pour the placed silk fibroin solution into a plastic tube, and place the plastic tube in a one-way freezer for freezing treatment. The bottom of the plastic tube is in direct contact with the copper plate. The refrigerant is a mixture of acetone and dry ice. The temperature of the refrigerant is The temperature is -70°C, the freezing time is 60min, and then the plastic tube is moved to the freeze dryer for 28h to obtain the scaffold;

[0053] (3) Cut the scaffold into a cylinder with a diameter of 10mm and a length of 2mm, treat it with methanol for 35min to make it no longer soluble in water, and freeze-dry;

[0...

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Abstract

The invention relates to a multi-channel conductive nerve graft and a preparation method thereof, and the method comprises the following steps: firstly, carrying out one-way freezing on a silk fibroin solution which is about to form gel after'concentration-dilution ', and freeze-drying to prepare an anisotropic SF stent with oriented fibers distributed on the surfaces of multiple channels; then, PEDOT: PSS is introduced into the surface of the channel of the SF stent in a dip-coating mode, so that the stent is endowed with certain electrical activity. The surface of the nerve graft is provided with an SF multi-channel structure with oriented fiber distribution, so that axon oriented growth of nerve cells can be induced, nerve repair can be better completed, and the existence of the electroactive PEDOT: PSS layer can promote growth and differentiation of the nerve cells and accelerate regeneration of nerve tissues.

Description

technical field [0001] The invention belongs to the technical field of nerve grafts, in particular to a multi-channel conductive nerve graft and a preparation method thereof. Background technique [0002] Nerve injury caused by sports, accidental injury or surgical resection is a frequently occurring disease in clinical surgery, and it is also one of the intractable diseases in the medical field. When there is a defect at the break, nerve grafts are needed to repair and induce tissue regeneration. Due to the lack of autologous nerve graft sources and mismatching sizes, nerve grafts are gradually used clinically to bridge severe nerve damage, and the repair effect is closely related to the structure and performance of nerve grafts. [0003] In general, neural grafts should have the basic properties of natural extracellular matrix. For example, it has good biocompatibility and degradability, suitable mechanical strength and porous structure for cell adhesion and migration. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/34A61L27/50A61L27/56A61L27/58C08J9/42C08L89/00C08L25/06C08L65/00
Inventor 孔倩张伟李晓静王国航孙冰冰康鹏管修东周文剑龙
Owner TIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD
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