A dragon fruit endophytic fungus hlb-1 and its application
A technology of HLB-1 and endophytic fungi, applied in the fields of application, fungi, microorganisms, etc., can solve the problem of no dragon fruit seed germination, etc., achieve broad market application prospects, promote seedling growth, and improve the effect of seed germination rate
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Embodiment 1
[0026] Example 1 Separation, purification and identification of dragon fruit endophytic fungus HLB-1
[0027] 1. Isolation and purification of strains
[0028] The dragon fruit endophytic fungus HLB-1 was isolated from the root of dragon fruit in Xixiangtang District, Nanning City, Guangxi. The method is as follows: rinse the collected pitaya root sample surface soil with sterile water, absorb the surface moisture with sterilized filter paper, and dry at room temperature. Use a sterile blade to cut the root system into 5mm long root segments, soak in 75% alcohol for 1 min, then soak in 5% sodium hypochlorite solution for 5 min, wash with sterile water 3 times, and use sterile filter paper to absorb the surface water of the root sample. Dry, transfer to PDA medium and place 3 root segments per dish. Take the sterile water washing solution for the last time of surface disinfection and apply it to the PDA plate to detect whether there is any bacterial contamination. After cult...
Embodiment 2
[0042]Embodiment 2 Effect verification of dragon fruit endophytic fungus HLB-1
[0043] 1. Preparation of strain fermentation broth of dragon fruit endophytic fungus HLB-1
[0044] Under sterile conditions, the dragon fruit endophytic fungus HLB-1 was cultured on a PDA plate at 28°C for 5 days, and 3 pieces of bacterial cakes with a diameter of 0.5 cm were taken at the edge of the colony, and then inoculated into sterilized and cooled 200ml liquid PDA respectively. In the medium, the control group was inserted into sterile agar blocks of the same size. The constant temperature was 28 °C, the shaking table was 180 r / min, and the culture medium was fully fermented for 10 d in the dark. After the fermentation, the liquid fermentation product was filtered with 5 layers of sterilized gauze to obtain a fermentation broth stock solution, which was stored at 4°C for later use.
[0045] 2. The effect of fermentation broth on the germination of dragon fruit seeds
[0046] 2.1 Select ...
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