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Acidic pea peptide as well as preparation method and application thereof

A pea peptide and acid technology, applied in the field of acid pea peptide and its preparation, can solve problems such as limiting the application of pea peptide, and achieve the effects of easy centrifugal separation, good separation effect and good sedimentation effect

Pending Publication Date: 2022-04-12
广州合诚实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the application scenarios of pea peptides, many oral liquids, dairy products, beverages, solid drinks and special medical foods are acidic conditions with a pH value of 3.5 to 4.5, and instability will limit the application of pea peptides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] (1) 50kg pea protein powder (Shandong Jindu Tallinn Food Co., Ltd.) is configured into 1000kg of protein solution with a concentration of 5% by mass with deionized water, and the pea protein powder is dispersed at a shear rate of 500 rpm;

[0053] (2) Use a concentration of 1mol L -1 The pH of the protein solution obtained in step (1) was adjusted to 7.0 with a sodium hydroxide solution, and 0.25kg alkaline protease (Alcalase alkaline protease, Novozymes (China) Investment Co., Ltd.) was added, and the enzymolysis time was 20h. The solution temperature is 40°C. After enzymatic hydrolysis, keep at 90°C for 60 minutes to inactivate the activity of alkaline protease, and then cool down to 50°C;

[0054] (3) Use a concentration of 3mol L -1The pH of the enzymolysis solution finally obtained in step (2) is adjusted to 4.85 by the citric acid solution;

[0055] (4) Centrifuge the enzymatic solution obtained in step (3), and get 740L of supernatant;

[0056] (5) Heat the s...

Embodiment 2

[0062] (1) 50kg of pea protein powder is configured into 1000kg of protein solution with a concentration of 5% by mass with deionized water, and the pea protein powder is dispersed at a shear rate of 500 rpm;

[0063] (2) Use a concentration of 10mol L -1 potassium hydroxide solution to adjust the pH of the protein solution obtained in step (1) to 8.95, add 1.0kg alkaline protease (COROLASE APC alkaline protease, British Union Enzyme Trading (Shanghai) Co., Ltd.), and the enzymatic hydrolysis time is 2h , the enzymatic hydrolysis temperature is 70°C. After enzymatic hydrolysis, keep at 100°C for 5 minutes to inactivate the activity of alkaline protease, and then cool down to 50°C;

[0064] (3) Use a concentration of 3mol L -1 The tartaric acid solution adjusts the pH of the enzymolysis solution finally obtained in step (2) to 5.46;

[0065] (4) Centrifuge the enzymatic solution obtained in step (3), and get 830L of supernatant;

[0066] (5) Heat the supernatant to 95°C and...

Embodiment 3

[0071] (1) 50kg of pea protein powder is configured into 1000kg of protein solution with a concentration of 5% by mass with deionized water, and the pea protein powder is dispersed at a shear rate of 500 rpm;

[0072] (2) Use a concentration of 5mol L -1 The pH of the protein solution obtained in step (1) was adjusted to 8.33 with sodium hydroxide solution, and 1.0kg alkaline protease (COROLASE APC alkaline protease, British Union Enzyme Trading (Shanghai) Co., Ltd.) was added, and the enzymolysis time was 6h , the enzymatic hydrolysis temperature is 55°C. After enzymatic hydrolysis, keep at 88°C for 30 minutes to inactivate the activity of alkaline protease, and then cool down to 50°C;

[0073] (3) Use a concentration of 3mol L -1 The pH of the enzymolysis solution finally obtained in step (2) is adjusted to 5.21 by the citric acid solution;

[0074] (4) Centrifuge the enzymatic solution obtained in step (3), and get 890L of supernatant;

[0075] (5) Heat the supernatant ...

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Abstract

The invention discloses acidic pea peptide as well as a preparation method and application thereof. The preparation method comprises the following steps: by taking pea protein powder as a raw material, decomposing pea protein into an oligopeptide mixture through an efficient biological enzymolysis technology, performing solid-liquid separation to obtain liquid, regulating the acid of the liquid with organic acid, performing high-temperature heat preservation, filtering, regulating the acid of the obtained filtrate until the pH value is 3.5-4.5 to obtain target pea peptide, and finally performing concentration, sterilization and spray drying processes to prepare a target product. The acidic pea peptide is high in stability and can be widely applied to the fields of products such as formula food for special medical purposes, oral liquid, dairy products and common solid beverages.

Description

technical field [0001] The invention belongs to the field of food, in particular to an acidic pea peptide and its preparation method and application. Background technique [0002] Pea is an excellent vegetable protein resource. Pea protein accounts for 23% to 25% of the dry pea mass. Pea protein is a good source of essential amino acids. Its composition is relatively balanced and its content of branched chain amino acids is high. The recommended mode is closer. In my country, pea protein is a by-product of producing pea vermicelli, and the manufacturers are concentrated in Shandong, such as Yantai Dongfang Protein, Shandong Shuangta, Shandong Liuliushun and other enterprises. Before 2010, pea protein was mainly used as feed. Since then, due to industrial upgrading, some large vermicelli manufacturers have extracted pea protein from wastewater as food raw materials. [0003] Compared with soybean protein, the functional properties of pea protein such as foaming, gelling, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34A23L33/18A23C9/152A23L2/39A23L2/66
Inventor 王才华周雪松曾建新蒋文真
Owner 广州合诚实业有限公司