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Friction nano-generator driven sensing system and method for bacterial detection

A nanogenerator and bacteria detection technology, applied in the field of microbial detection, can solve the problems of large environmental influence factors, environmental and industrial hazards, long detection time, etc. Effect

Active Publication Date: 2022-04-12
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Application Information

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Problems solved by technology

Therefore, the presence of SRB may cause environmental and industrial hazards
There are many detection methods for SRB, such as the most probable number method, biochemical detection, enzyme-linked immunosorbent assay and molecular biological technology, but these methods have the disadvantages of long detection time and large environmental factors.

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  • Friction nano-generator driven sensing system and method for bacterial detection
  • Friction nano-generator driven sensing system and method for bacterial detection
  • Friction nano-generator driven sensing system and method for bacterial detection

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Embodiment 1

[0035] Embodiment 1 This embodiment provides a sensor system for bacteria detection driven by a triboelectric nanogenerator, figure 1 The structure of the system is shown. Such as figure 1 As shown, the triboelectric nanogenerator-driven sensing system for bacteria detection of the present invention includes a triboelectric nanogenerator 100 , a resistive biosensor 200 , a variable resistor 300 and an LED lamp 400 . The triboelectric nanogenerator 100 is used to convert external mechanical energy into electrical energy to output electrical signals to an external circuit. The resistive biosensor 200 is electrically connected with the triboelectric nanogenerator 100, so that the triboelectric nanogenerator 100 supplies power to the resistive biosensor 200 for detecting sensing signals. The variable resistor 300 is connected in series with the resistive biosensor 200 to obtain a part of the voltage. The LED lamp 400 is connected to both ends of the variable resistor 300 in pa...

Embodiment 2

[0056] Embodiment 2 This embodiment provides a method for detecting bacteria using the sensor system for bacteria detection driven by the above-mentioned frictional nanogenerator. The method takes the detection of sulfate-reducing bacteria as an example. The process is as follows Figure 4 As shown, the specific steps are as follows:

[0057] (1) Soak the resistive biosensor in a series of known concentrations of SRB bacteria solution and incubate for 2 hours;

[0058] (2) Take out the above sensor, soak it in a solution of carbon nanotubes modified with concanavalin at a concentration of 0.15 mg / mL, and react for 2 hours;

[0059] (3) Take out the above sensor, gently rinse off the unconnected carbon nanotubes with deionized water, gently wipe off the water on the surface, and let it dry naturally;

[0060] (4) Connect the circuit, drive it with a triboelectric nanogenerator, and test the voltage value at both ends of the resistive biosensor; Figure 5 The relationship betw...

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Abstract

The invention belongs to the technical field of microbiological detection, and particularly relates to a bacterium detection system and method driven by a friction nano-generator. A sensing system driven by a friction nano-generator and used for detecting bacteria comprises the friction nano-generator used for converting external mechanical energy into electric energy and outputting the electric energy; the resistance-type biosensor is electrically connected with the friction nano-generator and is used for identifying and capturing target bacteria under the driving of the electric energy output by the friction nano-generator; the carbon nano tube is used for being combined with the target bacteria and causing the resistive biosensor to generate a sensing signal; the surfaces of the resistance-type biosensor and the carbon nanotubes are modified with biological recognition materials which are specifically combined with target bacteria. The sensor is driven by the friction nanometer generator, an external power supply is not needed, and the portability of the detection system is improved; the biological recognition material capable of being specifically combined with the target bacteria is adopted, so that the accuracy and sensitivity of the sensor are improved.

Description

technical field [0001] The invention belongs to the technical field of microorganism detection, and in particular relates to a bacteria detection system and method driven by a friction nanogenerator. Background technique [0002] Microbial corrosion can occur in almost all common materials. The corrosion loss caused by sulfate reducing bacteria (SRB) in microbial corrosion accounts for more than 1 / 2 of the total corrosion loss. SRB is an anaerobic bacterium, and the sulfide produced during its metabolism is extremely corrosive and toxic. Therefore, the existence of SRB may cause environmental and industrial hazards. There are many detection methods for SRB, such as the most probable number method, biochemical detection, enzyme-linked immunosorbent assay and molecular biological technology, but these methods have the disadvantages of long detection time and large environmental factors. Therefore, it is necessary to develop a highly sensitive and specific SRB detection meth...

Claims

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Application Information

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IPC IPC(8): G01N27/06G01N27/07H02N1/04
Inventor 王鹏周州
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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