Digital microscopic imaging system and microscopic examination method for biological sample

Abstract

The invention discloses a digital microscopic imaging system for a biological sample, which comprises a digital microscope, an illumination module and a focusing mechanism, and is characterized by further comprising an automatic focusing calculation unit for controlling the focusing mechanism to adjust the distance d between a sample surface and an object space focal plane of the digital microscope to be within a specified range, a digital microscope acquires a microscopic digital image of a sample, where n represents an object-side refractive index, lambda represents an illumination wavelength, and NA represents a numerical aperture of an objective lens of the digital microscope. The microscopic imaging system can be used for directly imaging a transparent or semitransparent sample which is not really dyed; the limitation caused by sample dyeing in a traditional microscopic examination method can be solved, the microscopic examination steps are simplified, and the microscopic examination time is shortened. Meanwhile, the system is easy to operate and more conforms to use habits of pathologists, biologists and the like.

Description

technical field [0001] The invention belongs to the field of microscopic imaging, and in particular relates to a digital microscopic imaging system and a microscopic inspection method for biological samples, such as blood, blood parasites, animal and plant tissue slices, oral epithelial cells, and the like. Background technique [0002] Microscopic examination (microscopic examination) is a common method for performing sample analysis and plays an important role in disease diagnosis. Since most biological samples, such as blood cells, cervical cells, cerebrospinal fluid, etc., are transparent or translucent samples, they cannot be observed by traditional microscopic imaging methods. In microscopic examination, it is usually necessary to use dyes (chemical dyes, fluorescent dyes, etc.) to stain the samples to mark the results of the samples. The stained samples were then visually inspected under a microscope by professionals in the field. This also brings the following limi...

AI Technical Summary

Problems solved by technology

[0003] 1. The dyeing steps are cumbersome and time-consuming;

[0004] 2. The dyeing operation is highly professional and needs to rely on trained professional and technical personnel;

[0005] 3. Differences in dyes and technical levels of different personnel will lead to differences in staining, which will affect the microscopic examination of samples;

[0006] 4. Microscopic examination of samples is carried out by professionals in the field through microscope vision, which is very time-consuming and energy-consuming; and the results of microscopic examination are affected by the subjective judgment of professionals in the field;

[0007] 5. The high reliance on professionals for staining and manual microscopic examination limits its application in emergency rescue scenarios and areas where medical resources are scarce

[0015] At the same time, in order to solve the problems that manual microscopic examination relies on professionals in the field, which is time-consuming and labor-intensive, and the results of microscopic examination are affected by the subjective judgment of professionals in the field, the imaging method needs to be combined with an intelligent algorithm based on machine learning for the acquisition of this method. Automatic data processing and analysis of digital images

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