Cell separation, extraction and processing device

Abstract

The invention relates to the technical field of bioengineering, in particular to a cell separation, extraction and processing device which comprises a bottom plate, a rotating part, a constant-temperature part and clamping parts, the rotating part is mounted at the upper end of the bottom plate, the constant-temperature part is mounted in the middle of the upper end of the rotating part, and a plurality of clamping parts which are uniformly distributed in the circumferential direction are mounted at the upper end of the rotating part. The problems that when cells in biological tissues are separated, a method of mixing trypsin and tissues and then performing rotary centrifugation is adopted, the cells of the tissues are easily separated by the method, and a mixed solution after centrifugal separation needs to be precipitated for a lot of time, so that the cell separation time is increased and the like can be solved. The suction mechanism adopted by the cell separation, extraction and processing device provided by the invention can block the test tube during cell centrifugal separation, so that the cells are prevented from being polluted by bacteria in air, the activity of the separated cells is ensured, and the success rate of cell separation is improved.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a cell separation and extraction processing device. Background technique [0002] Cell separation is to obtain individual cells, so that it is convenient to study the nucleus, mitochondria, Golgi apparatus, lysosomes and microsomes in the cell. Cell separation includes mechanical dispersion and digestion separation. The existing cell separation usually uses digestion Trypsin dispersion technique within separation method. [0003] When separating cells in biological tissues, trypsin is usually mixed with the tissue and then rotated and centrifuged. This method is easy to separate the cells of the tissue. The mixed solution after centrifugation needs a lot of time to settle, and then Then the liquid is separated from the cells, thereby increasing the time for cell separation and reducing the efficiency of cell separation. [0004] In the existing cell separation method, af...

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Problems solved by technology

[0003] When separating cells in biological tissues, trypsin is usually mixed with the tissue and then rotated and centrifuged. This method is easy to separate the cells of the tissue. The mixed solution after centrifugation needs a lot of time to settle, and then Then the liquid is separated from the cells, which increases the time of cell separation and reduces the efficiency of cell separation

[0004] In the existing cell separation method, after the centrifugation of the tissue, the separated liquid needs to be sucked and discharged. Due to the small diameter of the test tube, a larger suction device cannot enter the test tube, and the amount of the sucked liquid needs to be precisely controlled. The suction device is easy to suck out the cells and the liquid together, so the liquid in the test tube is usually sucked and discharged with a dropper. This method requires repeated operations many times, which makes the cell separation operation cumbersome.

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