Preparation method of methane normal saline
A technology for physiological saline and methane, applied in the field of medicine, can solve the problems of complicated preparation process, long time consumption and high preparation cost of methane saline solution, and achieve the effects of saving manpower and material resources, shortening time consumption and reducing preparation cost.
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[0023] Such as figure 1 As shown, the embodiment of the present invention provides a method for preparing methane saline. It includes:
[0024] S1. Degassing treatment: Use a syringe to extract other gases in the bagged saline package until only saline remains in the bagged saline package;
[0025] S2. Methane gas injection: inject methane gas into the degassed bagged saline package to fill the bagged saline package;
[0026] S3. Natural dissolution of methane gas: the bagged normal saline injected with methane gas is left to stand at low temperature for 24 hours, so that the methane gas is fully dissolved in the bagged normal saline before use.
[0027] In the present invention, the syringe is a medical syringe needle, and the bagged saline is 100ml bagged saline. In step S2, the methane gas injected into the degassed packaged saline package is 0-70ml, preferably, the methane gas injected into the degassed packaged saline package is 60ml, wherein, The methane gas is pure ...
Embodiment 1
[0030] test group:
[0031] Item preparation: Prepare 100ml bags of normal saline, pure methane gas with a purity of 99.99%, and a syringe set.
[0032] The method for preparing methane physiological saline of the present invention is used to prepare methane physiological saline.
[0033] S1. Degassing treatment: use a syringe to extract other gases in the 100ml bagged saline package until only saline remains in the bagged saline package;
[0034] S2. Methane gas injection: inject 60ml of pure methane gas into the degassed bagged saline package to fill the bagged saline package;
[0035] S3. Natural dissolution of methane gas: the bagged normal saline injected with methane gas was left to stand at low temperature for 24 hours, so that the methane gas was fully dissolved in the bagged normal saline, and methane normal saline with a methane concentration of 4.08 mmol / L was obtained. Data are recorded in Table 1.
[0036] Control group:
[0037] Preparation of Methane Physiol...
Embodiment 2
[0046] Animal experiment
[0047] 1. Experimental animals: C57 mice, body weight 20-25g.
[0048] 24 male mice were randomly divided into 3 groups: ① sham operation group (Control); ② liver ischemia-reperfusion injury group (IR); ③ ischemia-reperfusion injury + methane saline (dissolution method) treatment group ( IR+MS), wherein, the methane saline is prepared by the methane saline preparation method of the present invention. In the sham operation group, only laparotomy was performed without liver ischemia treatment. In the liver ischemia-reperfusion injury group, the liver was ischemia for 60 minutes and then reperfused for 6 hours. The treatment group was treated with 10ml / kg methane saline after the end of ischemia.
[0049] 2. Preparation of liver ischemia-reperfusion model:
[0050] C57 mice were anesthetized with chloral hydrate and fixed on the mouse board. A paramedian incision was made on the abdomen, and the liver was separated to expose the hepatic artery and p...
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