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Application of cyanidin in prevention and treatment of vascular calcification

A technology of cyanidin and vascular calcification, which is applied in the field of medicine, can solve the problems of lack of effective treatment of VC and achieve the effect of alleviating vascular calcification

Active Publication Date: 2022-04-26
中山大学附属第八医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still no effective treatment for VC
However, there is no report on cyanidin's relief of VC

Method used

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  • Application of cyanidin in prevention and treatment of vascular calcification
  • Application of cyanidin in prevention and treatment of vascular calcification
  • Application of cyanidin in prevention and treatment of vascular calcification

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0026] Cyanidin Chloride (Cyanidin Chloride) of different concentrations of experimental example 1 is to the toxic effect of HASMC

[0027] Use cell counting kit 8 (CCK-8; Dojindo Co, Kumamoto, Japan) to detect chlorinated cyanidins [molecular formula: C 15 h 11 ClO 6 , purchased from Sigma (Sigma-Aldrich)], cytotoxicity to HSAMC (purchased from ATCC company) First, transfer the 5th generation HSAMC to a 96-well plate (5×10 per square centimeter) 3 In cells), cyanidin chloride was prepared with DMSO solution and added to high glucose medium containing 10% fetal bovine serum to release cyanidin solutions with different concentrations (0, 12.5, 25, 50 and 100 μM ), and then add 100uL cyanidin chloride solution to each well, and treat the cells for 24 hours and 48 hours respectively. And set blank group (no cell without drug, only add 100uL serum medium) and control group (add equal amount of cells, no drug), when the cells grow to 80% of the 96-well plate, add phosphate buffe...

Embodiment 2

[0030] The mitigation effect of embodiment 2 cyanidin to HASMC calcification

[0031] HSAMC was seeded in a 12-well plate at passage 6, and calcification was induced when the cell growth reached 80% density. While adding high pi solution (2.8mM), 0, 12.5, 25, 50 and 100μM chlorine were added respectively. Calcium cyanidin was induced for a total of 7 days, and the medium was replaced every 2 days, and corresponding intervention treatments were added. After 7 days of calcification induction, alizarin red staining was performed, and the culture medium in the culture dish was sucked out, and PBS was gently Rinse three times, fix with 4% paraformaldehyde for 30min, then gently rinse with PBS three times, after absorbing the PBS, add 100uL of Alizarin Red staining solution to each well, stain for about 20 minutes, absorb the staining solution, rinse gently with distilled water for three times, and finally Perform microscope observation and take pictures, the results are as follows ...

Embodiment 3

[0033] Example 3 The preventive effect of cyanidin on high phosphorus (Pi) diet-induced calcification mice

[0034] (1) Establishment of CKD vascular calcification model in mice

[0035] Establishment of chronic kidney disease model in mice induced by high phosphorus and high adenine diet: 6-8 weeks old wild-type C57BL / 6J male mice (purchased from Animal Experiment Center of Sun Yat-Sen University) were selected. The CKD vascular calcification model (belonging to endometrial calcification) was constructed by feeding with a special feed containing high phosphorus and high adenine containing 0.2% adenine and 1.2% phosphoric acid, and the total feeding time was 12 weeks.

[0036] (2) Experimental method

[0037] 1) Effect of cyanidin on body weight of mice with calcification induced by high phosphorus diet

[0038] 36 C57BL / 6J male mice weighing 23-25g were randomly divided into 3 groups, 12 in each group, normal diet + sodium chloride (5mg / kg) tail vein injection group (ND), h...

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PUM

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Abstract

The invention belongs to the technical field of medicines, particularly relates to application of cyanidin in prevention and treatment of vascular calcification, and discloses application of cyanidin in prevention and treatment of vascular calcification in order to find a new compound for treatment of VC and provide a better prevention and treatment scheme for VC patients. The cyanidin treatment is carried out in the calcified male mouse induced by high Pi and in the calcification induction process of HASC, and it is found that cyanidin can relieve vascular calcification and is expected to be applied to prevention and treatment of vascular calcification diseases. The invention not only provides a new application of cyanidin, but also provides a new thought for treating vascular calcification.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of cyanidin in preventing and treating vascular calcification. Background technique [0002] Chronic kidney disease (CKD) is an important public health problem, with 697.5 million CKD patients worldwide in 2017. CKD has caused 1.2 million deaths worldwide and is expected to increase to 2.2 million by 2040. Cardiovascular disease is the main cause of early death in CKD patients, and vascular calcification (VC) is an important culprit. But there is still no effective treatment for VC. Therefore, there is a need for new compounds to be used in the treatment of VC to provide better prevention and treatment options for VC patients. [0003] Studies have shown that the bioactive substances of flavonoids, especially anthocyanins, have health benefits. Anthocyanins are composed of cyanidin, peonidin, delphinidin, pelargonidin, pemnidin and malvidin. Glyc...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P9/00A61P13/12
CPCA61K31/352A61P9/00A61P13/12
Inventor 黄辉尹力张郑志鹏
Owner 中山大学附属第八医院
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