Application of corn miRNA

A corn, zma-unmir4 technology, applied in applications, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of mildew and rot of cob and grain, infecting corn grain, affecting corn yield, etc.

Active Publication Date: 2022-04-26
HENAN AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

During the grain filling stage of corn, Fusarium pseudoverticillium will infect corn kernels or cobs, causing mold and rot of cobs and kernels, thus seriously affecting corn yield

Method used

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  • Application of corn miRNA
  • Application of corn miRNA
  • Application of corn miRNA

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Embodiment 1

[0040] (1) The application of the corn miRNA of this embodiment, the corn miRNA is named zma-unmiR4, and the zma-unmiR4 responds to the infection of Fusarium verticillioides (Fusarium verticillioides), and is used to regulate the plant's response to Verticillium Fusarium verticillioides resistance, the nucleotide sequence of the mature transcript of zma-unmiR4 is shown in SEQ ID NO:1.

[0041] Take freshly germinated N6 seeds and Colombian wild-type Arabidopsis thaliana grown on MS board for 10 days, and extract total RNA. Trizol extraction method is used for the extraction of total RNA: before sampling, burn the tweezers, scissors, and blades required for sampling with the flame of an alcohol lamp , take down the materials used for RNA extraction, wrap them in tinfoil, and put them into liquid nitrogen for quick freezing. Samples that are not extracted immediately can be transferred to a -80°C ultra-low temperature freezer for later use. Take an appropriate amount of tissue a...

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Abstract

The invention provides application of corn miRNA (micro ribonucleic acid), the corn miRNA is named as zma-unmiR4, the zma-unmiR4 responds to infection of fusarium verticillioides and is used for regulating and controlling the resistance of a plant to the fusarium verticillioides, and a mature transcript nucleotide sequence of the zma-unmiR4 is as shown in SEQ ID NO: 1. The miRNA (zma-unmiR4) of the maize can respond to infection of fusarium verticillioides, and after the fusarium verticillioides is inoculated, a primary transcript of the zma-unmiR4 is down-regulated in a disease-resistant maize inbred line BT-1, and is up-regulated in a susceptible maize inbred line N6. The zma-unmiR4 is subjected to heterologous overexpression in arabidopsis thaliana, so that the resistance of a plant to fusarium verticillioides can be regulated and controlled.

Description

technical field [0001] The invention belongs to the technical field of corn miRNA, and in particular relates to an application of corn miRNA. Background technique [0002] Fusarium verticillioides is a pathogenic fungus that can cause many crop epidemic diseases, especially for corn (Zea mays L.). Corn is one of the most important food crops in my country, and it is also an important feed and industrial raw material. Its production plays a pivotal role in my country's food security. However, during the whole growth and development of maize, it is often attacked by Fusarium pseudoverticillium, and then various diseases such as seedling rot, stem rot, ear rot and seed rot occur. In the early stage of corn seedling development, infection with Fusarium pseudoverticillium will affect the germination rate of corn, the growth of seedling population, the height of plants, etc., and in severe cases, the seedlings will wither and die. In the middle stage of maize development, the in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/66C12N15/84A01H5/00A01H6/20
CPCC12N15/113C12N15/8282C12N15/66C12N2310/141Y02A40/146
Inventor 徐玉芳张会勇李涛汪仁洁
Owner HENAN AGRICULTURAL UNIVERSITY
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