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Ginseng adventitious root induction method and application of ginseng adventitious root extract in cosmetics

A root extract and ginseng technology, applied in the ginseng adventitious root induction method and the application field of the extract in cosmetics, can solve the problems of plant tissue withering, plant tissue damage, difficult cleaning, etc., and achieve the effect of reducing tissue infection problems

Pending Publication Date: 2022-04-29
上海瑞帝安生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is that the ginseng root tissue is not only difficult to clean in the surface disinfection stage due to the many threads and silt on the surface, but also has a high bacterial contamination rate. Liquid will cause excessive damage to plant tissue, causing problems such as dead plant tissue, the present invention provides a method for inducing adventitious roots of ginseng, which can reduce the problem of tissue infection in the existing method of inducing ginseng adventitious roots with ginseng roots and make full use of ginseng seedlings The nutritional value of stem and leaf tissue, using the stem or leaf tissue in ginseng seedlings to induce ginseng adventitious roots, and confirming the conditions of the ginseng adventitious root induction medium, preparing the ginseng adventitious root extract from the obtained ginseng adventitious root tissue, and at the same time, Based on the rich bioactive components of ginseng, the application of ginseng adventitious root extract as a raw material with antioxidant activity in the field of cosmetics was further evaluated to solve the defects caused by the existing technology

Method used

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  • Ginseng adventitious root induction method and application of ginseng adventitious root extract in cosmetics
  • Ginseng adventitious root induction method and application of ginseng adventitious root extract in cosmetics
  • Ginseng adventitious root induction method and application of ginseng adventitious root extract in cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1, obtaining ginseng seedling stem tissue or ginseng seedling leaf tissue, rinse the surface of the ginseng seedling stem tissue or ginseng seedling leaf tissue in tap water, then wash in 70% ethanol solution for 30s-60s, and then soak in 5% bleach After the dose, shake at a speed of 70rpm for 5min, and then rinse with sterilized distilled water for 4-5 times in a sterile environment;

[0038]Then, the ginseng seedling stem tissue or ginseng seedling leaf tissue is cut into tissue segments with a length of 0.5-1 cm, and then inoculated into a ginseng adventitious root induction medium with a pH value range of 5.6-5.9. The adventitious roots of ginseng are induced by culturing in the dark for 3-6 weeks, wherein the adventitious root induction medium of ginseng comprises SH medium (Schenkand Hildebrandt) with a concentration of 6.075g / L, sucrose with a concentration of 15g / L, and agar with a concentration of 6g / L , indole butyric acid (IBA) with a concentration of...

Embodiment 2

[0046] Example 2: Obtain ginseng seedling stem tissue or ginseng seedling leaf tissue, rinse the surface of the ginseng seedling stem tissue or ginseng seedling leaf tissue in tap water, then wash in 70% ethanol solution for 30s-60s, and then soak in 10% bleach After the dose, shake at a speed of 100rpm for 15min, and then rinse with sterilized distilled water for 4-5 times in a sterile environment;

[0047] Then, the ginseng seedling stem tissue or ginseng seedling leaf tissue is cut into tissue segments with a length of 0.5-1 cm, and then inoculated into a ginseng adventitious root induction medium with a pH value range of 5.6-5.9. The adventitious roots of ginseng are induced by cultivating in the dark for 3-6 weeks, wherein the ginseng adventitious root induction medium comprises SH medium (Schenkand Hildebrandt) with a concentration of 12.15g / L, sucrose with a concentration of 20g / L, and agar with a concentration of 7g / L , indole butyric acid (IBA) with a concentration of...

Embodiment 3

[0055] Example 3: Obtaining ginseng seedling stem tissue or ginseng seedling leaf tissue, rinsing the surface of the ginseng seedling stem tissue or ginseng seedling leaf tissue in tap water, then washing in 70% ethanol solution for 30s-60s, and then soaking in 15% bleach After the dose, shake at a speed of 130rpm for 25min, and then rinse with sterilized distilled water 4-5 times in a sterile environment;

[0056] Then, the ginseng seedling stem tissue or ginseng seedling leaf tissue is cut into tissue segments with a length of 0.5-1 cm, and then inoculated into a ginseng adventitious root induction medium with a pH value range of 5.6-5.9. The adventitious roots of ginseng are induced by culturing in the dark for 3-6 weeks, wherein the adventitious root induction medium of ginseng comprises SH medium (Schenkand Hildebrandt) with a concentration of 24.3g / L, sucrose with a concentration of 50g / L, and agar with a concentration of 8g / L , indole butyric acid (IBA) with a concentra...

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Abstract

The invention discloses a ginseng adventitious root induction method which comprises the following steps: acquiring ginseng seedling tissues and disinfecting; the disinfected ginseng seedling tissue is cut into tissue sections, the tissue sections are inoculated into a ginseng adventitious root induction culture medium, ginseng adventitious roots are induced, and the ginseng adventitious root induction culture medium comprises an SH culture medium with the concentration being 6.075-24.3 g / L, saccharose with the concentration being 15-50 g / L, agar with the concentration being 6-8 g / L and indolebutyric acid with the concentration being 0.5-3.5 mg / L; the tissue infection problem in the existing ginseng root adventitious root induction can be reduced, the nutritional value of ginseng seedlings is fully utilized, stem or leaf tissues in the ginseng seedlings are used for induction of ginseng adventitious roots, the conditions of the ginseng adventitious root induction culture medium are confirmed, and the ginseng adventitious root induction culture medium has the advantages that the ginseng adventitious root induction culture medium has a good application prospect on the basis of rich bioactive components of ginseng. The application of the ginseng adventitious root as a raw material with antioxidant activity in the field of cosmetics is further evaluated.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for inducing adventitious roots of ginseng and the application of the extract in cosmetics. Background technique [0002] Ginseng (Panax ginseng) belongs to the genus Panax notoginseng and the purslane family. Due to its excellent pharmacological effects, it has been widely used as a herbal plant in East Asia. Its history of use has been more than 2,000 years. It plays an important role, and the physiological activities mainly include metabolism regulation, blood pressure regulation, immune function regulation, anti-tumor, anti-oxidation and anti-aging effects. [0003] However, ginseng needs to be precisely adjusted for environmental conditions such as temperature, luminosity and humidity during the breeding process, and it takes a long period of time to obtain ginseng of a certain size. Ginseng tissue culture using tissue culture technology can take a smal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A61K8/9789A61Q19/08
CPCA01H4/00A01H4/002A61K8/9789A61Q19/08A61K2800/522
Inventor 张贤尽齐秋月耿懂懂黄淳锡朴寅纪
Owner 上海瑞帝安生物科技有限公司
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