Application of N-acetylcysteine in preparation of preparation for preventing or treating venous outflow disorder and related diseases thereof
A technology for acetylcysteine, barriers, applied in the field of biomedicine
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Embodiment 1
[0042] 1. Establishment of animal (rodent) model of cerebral venous outflow disorder;
[0043] Select adult (6-8 weeks old) female / male SD rats, and divide the SD rats into two groups:
[0044] (1) Sham operation control group (Sham group);
[0045] (2) According to the anatomical characteristics of the head and jugular veins of rodents, an animal model in line with the pathogenesis of human cerebral venous outflow disorder was designed, that is, the simultaneous ligation of the posterior glenoid vein and the internal jugular vein (JVL group). The specific process was as follows: Rats were anesthetized with halothane, and the isoflurane concentration was maintained between 1.5% and 2% during the procedure. Each rat was placed on a thermal pad, and the hair on the neck and front of the ears was removed with a shaver. Under the operating microscope, adjust the field of view and focus of the microscope, perform routine disinfection, and first make a longitudinal skin incision a...
Embodiment 2
[0058] Rat NAC feeding and behavioral testing.
[0059] Rats were randomly divided into the following four groups (n=6 in each group): control group (Sham), operation group (JVL), control+NAC group (Sham+NAC), operation+NAC group (JVL+NAC), four Rats in the same group underwent the same anesthesia and analgesia protocol.
[0060] i) The rats in the Sham group underwent sham surgery, and the rats in the JVL group underwent cerebral venous outflow disorder model construction. After the operation, these rats were given free access to water and food;
[0061] ii) The rats in the Sham+NAC group underwent sham surgery, and the rats in the JVL+NAC group underwent cerebral venous outflow disorder model construction. After the operation, NAC was added to the daily drinking water of the rats at a concentration of 100 mg / kg / day, and the rats were given free access to water and food;
[0062] After 1 month of NAC feeding, the rats in all groups were subjected to behavioral tests such a...
Embodiment 3
[0066] Detection of NAC content in human cerebrospinal fluid.
[0067] (1) Collect cerebrospinal fluid from patients diagnosed with cerebral venous outflow disorder and non-central nervous system damage respectively, use ordinary cerebrospinal fluid collection tubes according to routine collection operations, keep about 1 mL of cerebrospinal fluid, and store it in a -80°C refrigerator;
[0068] The following sampling standards were adopted for the collection of CSF samples from patients with non-central nervous system damage.
[0069] Inclusion criteria:
[0070] (a) There are no special restrictions on age and gender;
[0071] (b) Patients with non-brain, spinal cord and other nervous system damage (such as hernia, fracture, etc.).
[0072] Exclusion criteria:
[0073] (a) Parkinson's disease, Alzheimer's disease, epilepsy, malignant tumor of any system;
[0074] (b) There are venous sinus thrombosis, intracranial infection, vasculitis and other rheumatic immune diseases;...
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