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Manufacturing method of macrobrachium rosenbergii gonad tissue frozen section

A technique of frozen sectioning of Macrobrachium rosenbergii, which is applied in the biological field, can solve problems such as unclear sectioning, and achieve the effect of complete sectioning, complete tissue morphology and high sectioning efficiency

Pending Publication Date: 2022-04-29
徐海晶
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  • Application Information

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Problems solved by technology

Compared with the paraffin section technology, the present invention has higher sectioning efficiency, and has more complete tissue morphology and section effect compared with the conventional frozen section technique of fresh tissue directly frozen section, and solves the problem of sectioning made of high-fat tissue unclear question

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Embodiment Construction

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0025] A method for making frozen section of Macrobrachium rosenbergii gonad tissue, the steps are as follows:

[0026] (1) Pre-treatment of slides for frozen sections: wash the slides in deionized water, rinse with absolute ethanol, and then air-dry naturally. Spread a layer of poly-lysine evenly before use;

[0027] (2) After frozen anesthesia of Macrobrachium rosenbergii, the gonad tissue (ovary, testis) was dissected and immediately placed in 4% paraformal...

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Abstract

The invention discloses a manufacturing method of a macrobrachium rosenbergii gonad tissue frozen section, a method for manufacturing the macrobrachium rosenbergii gonad tissue frozen section through a frozen section technology and application of the macrobrachium rosenbergii gonad tissue frozen section. The macrobrachium rosenbergii gonad tissue freezing and slicing method mainly comprises the steps of pretreatment of a glass slide before slicing, fixation of tissues by 4% paraformaldehyde, tissue embedding pretreatment, embedding, freezing and slicing and HE dyeing. Compared with a paraffin sectioning technology, the method has higher sectioning efficiency, and compared with a conventional freezing sectioning technology, the method has a more complete tissue form.

Description

【Technical field】 [0001] The invention relates to the field of biotechnology, in particular to a method for preparing cryosections of gonad tissue of Macrobrachium rosenbergii. 【Background technique】 [0002] Macrobrachium rosenbergii ( Macrobrachium rosenbergii ) is a large freshwater economic shrimp native to Southeast Asia. Because of its fast growth and good nutrition, it is also known as the king of freshwater shrimp. It has been widely introduced as an economic aquatic species by countries all over the world. One of the three major shrimp species. [0003] At present, the sectioning technique of the gonad tissue of Macrobrachium rosenbergii is often realized by paraffin sectioning. However, paraffin-sectioned tissues need to undergo a large amount of pre-embedding and pre-staining reagents, and the steps are cumbersome, time-consuming and labor-intensive. Moreover, for detection schemes such as in situ hybridization that have high requirements for samples, due to th...

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Application Information

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IPC IPC(8): G01N1/28G01N1/30G01N1/36G01N1/42
CPCG01N1/286G01N1/30G01N1/36G01N1/42G01N2001/2873
Inventor 徐海晶
Owner 徐海晶
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