Microcapsule powder preparation process for improving stability of probiotics and probiotic particles
A preparation process and technology of microcapsule powder, applied in the field of probiotics, can solve the problems of high bacterial mortality, affect product quality, low viable bacteria content, etc., to improve survival rate, promote product quality and production efficiency, and reduce water consumption. effect of activity
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Embodiment 1a
[0061] Embodiment 1a, a preparation process of microcapsule powder for improving the stability of probiotics, comprising the following steps:
[0062] S1: Add 0.115kg of palmitic acid and 0.115kg of stearic acid into a stirring tank, mix and melt, add 3kg of Lactobacillus acidophilus R0052 (50 billion cfu / g), 3kg (25 billion cfu / g) of Bifidobacterium longum R175 , stirred for 20 minutes, then added 40 kg of lyoprotectant, and continued to stir for 30 minutes to obtain an emulsion;
[0063] S2: Put the emulsion in the freeze dryer, turn on the refrigeration equipment, and drop it to -80°C within 1h, pre-freeze at -80°C for 6h, vacuumize and dry after the pre-freezing is completed, the vacuum degree is 20Pa, Turn off the refrigeration equipment, let the temperature naturally rise to room temperature (25±2°C) to obtain a freeze-dried block; take out the freeze-dried block and pulverize it to obtain a semi-finished microcapsule powder;
[0064] S3: Use a mixed fatty acid composed...
Embodiment 2a
[0066] Embodiment 2a, a kind of microcapsule powder preparation process that improves the stability of probiotics, comprises the following steps:
[0067] S1: Add 0.2㎏ palmitic acid and 0.1㎏ stearic acid into a stirring tank, mix and melt, add 3kg of Lactobacillus rhamnosus HN001 (50 billion cfu / g), 3kg (25 billion cfu / g) of Bifidobacterium lactis For HN019, stir for 20 minutes, then add 30 kg of lyoprotectant, and continue stirring for 30 minutes to obtain an emulsion;
[0068] S2: Put the emulsion in a freeze dryer, turn on the refrigeration equipment, and drop it to -60°C within 1 hour, pre-freeze at -60°C for 8 hours, vacuumize and dry it after the pre-freezing is completed, and the vacuum degree is 15Pa. Turn off the refrigeration equipment, let the temperature naturally rise to room temperature (25±2°C) to obtain a freeze-dried block; take out the freeze-dried block and pulverize it to obtain a semi-finished microcapsule powder;
[0069] S3: Use a mixed fatty acid compo...
Embodiment 3a
[0071] Embodiment 3a, a kind of preparation process of the microcapsule powder that improves the stability of probiotics, comprises the following steps:
[0072] S1: Add 0.115㎏ palmitic acid and 0.115㎏ stearic acid to the pretreatment box 1, mix and melt, add 3㎏ Lactobacillus acidophilus R0052 (50 billion cfu / g), 3㎏ (25 billion cfu / g) long bifido Bacillus R175, stirred for 10 minutes, then added 40kg of lyoprotectant, and continued to stir for 20 minutes to obtain an emulsion; spray liquid carbon dioxide with a temperature of -40°C on the surface of the emulsion, and then perform thermal expansion treatment at 70°C to obtain pretreatment Emulsion;
[0073] S2: Transfer the pretreated emulsion to the freeze dryer, turn on the refrigeration equipment, and drop to -80°C within 1.5h, prefreeze at -80°C for 6h, vacuumize and dry after the prefreeze Temperature is 20Pa, turn off the refrigeration equipment, make the temperature naturally warm up to room temperature (25 ± 2 ℃), obta...
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