Application of SIRTINOL in preparation of medicine for preventing and treating coronavirus

A coronavirus and antiviral drug technology, applied in antiviral agents, drug combinations, pharmaceutical formulas, etc., can solve the problems of curing new coronavirus pneumonia and poor curative effect, and achieve high therapeutic index, low effective concentration, and increased effect Effect

Pending Publication Date: 2022-05-06
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, so far there is still no specific drug that can cure the new type of coronavirus pneumonia
Most of th

Method used

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  • Application of SIRTINOL in preparation of medicine for preventing and treating coronavirus
  • Application of SIRTINOL in preparation of medicine for preventing and treating coronavirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Construction, Production and Verification of Pseudovirus Particles Modified by New Coronavirus S Protein

[0037] In order to simulate the natural process of SARS-CoV-2 using the S protein to recognize the ACE2 receptor to infect host cells, a pseudovirus with a replication-deficient lentivirus as the core and modified with the SARS-2-S protein was constructed particles. The construction method adopts the method published in the article Evaluate severe acute respiratory syndrome coronavirus 2 infectivity by pseudoviral particles, J Med Virol. August 10, 2020.

[0038] First, the DNA sequence of the SARS-2-S protein was modified without changing its amino acid sequence by means of codon optimization, which facilitated the massive expression of SARS-2-S in 293T cells ( figure 1 A). Subsequently, using the replication-deficient HIV lentivirus commonly used in the laboratory as the core of the pseudovirion, the three-plasmid expression system was transfected in ...

Embodiment 2

[0040] Example 2 Cell Biology Platform Screening Clinical Drugs to Inhibit SARS-2-S Pseudovirus Particle Infection

[0041] Utilize the SARS-2-S pseudovirion in vitro infection model (Luciferase reporter system and immunofluorescent staining localization system) that embodiment 1 builds, carry out SIRTINOL in vitro cell biology verification. ACE2-GFP stably transfected 293T cells were inoculated in 96-well plates, and the cells were pretreated with different concentrations of SIRTINOL for 2 hours, and then added with SARS-2-S pseudovirion particles to infect for 3 hours, and then the supernatant was removed and replaced with fresh complete medium. After 40 hours of infection, the cells were lysed using the Luciferase Assay System (Promega) and the reaction substrate was added. Glomax 96 was used to measure the luciferase luminescence intensity, which is proportional to the infection efficiency of virus particles.

[0042] The results of Luciferase activity test showed that SIR...

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Abstract

The invention relates to an application of SIRTINOL in preparation of a medicine for preventing and treating coronavirus. Specifically, the invention discloses application of SIRTINOL or a pharmaceutically acceptable salt, an isotope, a stereoisomer, a mixture of stereoisomers, a tautomer, an ester, an amide or a prodrug thereof in preparation of drugs for preventing and treating diseases caused by coronavirus. The coronavirus is a novel coronavirus SARS-Cov-2, a novel coronavirus SARS-CoV, a novel coronavirus HCoV 229E, a novel coronavirus NL63, a novel coronavirus OC43, a novel coronavirus HKU1 and a novel coronavirus MERS-CoV. The diseases caused by the coronavirus are pneumonia or complications of the pneumonia caused by any one of SARS-Cov-2 virus, SARS-CoV virus, HCoV 229E virus, NL63 virus, OC43 virus, HKU1 virus or MERS-CoV virus. The invention confirms that SIRTINOL has a novel coronavirus inhibition effect on new coronal pneumonia for the first time, is low in half effective concentration and has great potential.

Description

technical field [0001] The invention belongs to the field of medicines, and in particular relates to the application of SIRTINOL in the preparation of medicines for preventing and treating coronaviruses. Background technique [0002] Novel coronavirus pneumonia (Corona Virus Disease 2019) is an infectious disease caused by a new type of coronavirus (SARS-Cov-2) infecting the human body. Its symptoms mainly include fever, fatigue, dry cough, dyspnea and renal failure, etc. 2020, 395(10223):507-513; The Lancet, 2020, 395(10223):497-506]. Coronaviruses belong to the genus Coronavirus in the family Coronaviridae. Viruses of the genus Coronavirus are positive-strand single-stranded RNA viruses with an envelope (envelope), with a diameter of about 80-120nm. Its genetic material is the largest among all RNA viruses, and generally only infects humans, mice, pigs, cats, and dogs. , Avian vertebrates. Coronaviruses were first isolated from chickens in 1937. The shape of coronaviru...

Claims

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Application Information

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IPC IPC(8): A61K31/166A61K45/06A61P31/14A61P11/00
CPCA61K31/166A61K45/06A61P31/14A61P11/00A61K2300/00
Inventor 袁曙光李红昌陈显翀李红春崔文强邹荣峰刘科
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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