Enrichment and separation method of low-temperature aerobic denitrifying bacteria

Abstract

The invention discloses a method for enriching and separating low-temperature aerobic denitrifying bacteria, which comprises the following steps: S1, directionally collecting sludge with the temperature maintained at 3-5 DEG C in a river water body to obtain aerobic denitrifying bacteria, S2, screening the obtained aerobic denitrifying bacteria to obtain ten aerobic denitrifying bacteria with the highest deoxidation efficiency, and S3, respectively putting the culture solution and the screened aerobic denitrifying bacteria into the ten groups of culture dishes, and carrying out aeration enrichment on the culture dishes. According to the enrichment and separation method of the low-temperature aerobic denitrifying bacteria, the low-temperature aerobic denitrifying bacteria in a cultured solid culture dish are extracted, the extraction method of the low-temperature aerobic denitrifying bacteria is a centrifugal extraction method, the transfer time of the centrifugal extraction method in the extraction process is 130-155 r. Min, the temperature is kept at 30 DEG C, and the concentration of the low-temperature aerobic denitrifying bacteria is controlled to be 10-20%. And carrying out aeration culture to obtain a large amount of low-temperature aerobic denitrifying bacteria with strong activity for degrading COD and nitrate nitrogen, thereby realizing enrichment and separation of the low-temperature aerobic denitrifying bacteria.

Description

technical field [0001] The invention relates to the technical field of microorganism culture, in particular to a method for enriching and separating low-temperature aerobic denitrifying bacteria. Background technique [0002] Aerobic denitrifying bacteria are a type of denitrifying bacteria that use the action of aerobic denitrifying enzymes to perform denitrification under aerobic conditions. Adding the cultured low-temperature aerobic denitrifying bacteria to sewage treatment facilities can improve the effect of winter sewage denitrification treatment, and the low-temperature aerobic denitrifying bacteria can effectively and quickly remove the nitrate inside the polluted water body. [0003] Such as the patent CN201510124201.7 low-temperature aerobic denitrifying bacteria and its application. A low-temperature aerobic denitrifying bacterium, which can grow at 15°C and achieve denitrification under aerobic and low carbon-to-nitrogen ratio conditions. Under aerobic conditi...

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Problems solved by technology

[0007] The purpose of the present invention is to provide a method for the enrichment and separation of low-temperature aerobic denitrifying bacteria, so as to solve the problem that the process of extracting low-temperature aerobic denitrifying bacteria in the above-mentioned background technology is relatively cumbersome and cannot effectively treat low-temperature aerobic denitrifying bacteria. and the current low-temperature aerobic denitrifying bacteria culture method is simple, simply cultivating aerobic denitrifying bacteria at low temperature, without considering the activity of aerobic denitrifying bacteria at low temperature, resulting in aerobic denitrification The problem of low activity of bacteria at low temperature

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