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Bacillus inoculant for preventing and treating fusarium wilt of cucumber as well as preparation method and application of bacillus inoculant

A technology of Bacillus subtilis and Bacillus, applied in the field of microbial preparations, can solve the problems of not mentioning the effect of cucumber seeds, the effect of cucumber seed germination and seedling growth, etc., and achieve the benefits of increasing production and income, promoting germination, and promoting seedlings The effect of growth

Active Publication Date: 2022-05-10
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But it did not mention the effect on cucumber seed germination and seedling growth, especially did not mention the effect on cucumber seed germination

Method used

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  • Bacillus inoculant for preventing and treating fusarium wilt of cucumber as well as preparation method and application of bacillus inoculant
  • Bacillus inoculant for preventing and treating fusarium wilt of cucumber as well as preparation method and application of bacillus inoculant
  • Bacillus inoculant for preventing and treating fusarium wilt of cucumber as well as preparation method and application of bacillus inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Composition of Bacillus inoculum:

[0052] The total bacterial count is 1.06×10 10 cfu / mL;

[0053] Bacillus subtilis ZR-2 8.0×10 9 cfu / mL;

[0054] Bacillus amyloliquefaciens ZR-3 2.6×10 9 cfu / mL.

[0055] The preparation method of above-mentioned bacillus bacterial agent is as follows:

[0056] 1). Incline culture: Bacillus subtilis ZR-2, Bacillus amyloliquefaciens ZR-3 four kinds of original strains were inoculated on the solid medium under sterile conditions, cultured at 35°C for 1 day to activate the strains ;

[0057] 2). First-level seed cultivation: Inoculate the activated strains in step 1) in liquid medium under sterile conditions, Bacillus subtilis ZR-2 and Bacillus amyloliquefaciens ZR-3 at 35°C at 150r / min Cultivate on a shaking table for 24 hours, until the optical density of each bacterial suspension is OD 600 When the values ​​reached 3.0, the cultivation was stopped, and the first-grade seeds were obtained;

[0058] 3).Secondary seed culture: I...

Embodiment 2

[0066] Composition of Bacillus inoculum:

[0067] The total number of bacteria is 1.2×10 10 cfu / mL;

[0068] Bacillus subtilis ZR-2 9.0×10 9 cfu / mL;

[0069] Bacillus amyloliquefaciens ZR-3 3.0×10 9 cfu / mL;

[0070] The preparation method of above-mentioned bacillus bacterial agent is as follows:

[0071] 1). Incline culture: two original strains of Bacillus subtilis ZR-2 and Bacillus amyloliquefaciens ZR-3 were respectively inoculated on solid medium under sterile conditions, and cultured at 35°C for 1 day to activate the strains ;

[0072] 2). First-level seed cultivation: Inoculate the activated strains in step 1) in the liquid medium under sterile conditions, Bacillus subtilis ZR-2 and Bacillus amyloliquefaciens ZR-3 at 35°C at 200r / min Cultivate on a shaker for 1 day, until the optical density of each bacterial suspension is OD 600 When the values ​​reached 4.0, the cultivation was stopped, and the first-grade seeds were obtained;

[0073] 3).Secondary seed cultu...

Embodiment 3

[0079] The influence of embodiment 3 on cucumber seed germination

[0080] Cucumber variety to be tested: Jinyi No. 1

[0081] Method: Select the seeds of the test crops with full grains, and use 15% concentration of H 2 o 2 The solution was surface sterilized and rinsed 3 times with sterile water. Get a certain amount of cucumber seeds respectively and put into the dilution 10 that embodiment 1 obtains 7 cfu mL -1 Soak the seeds in Bacillus inoculum for 6 hours, take a sterilized petri dish, spread a layer of absorbent cotton on the bottom, place 20 crop seeds evenly on it, and cover with a layer of absorbent cotton, add water dropwise to soak the absorbent cotton completely, place Cultivate in dark at 25°C, and take the crop seeds soaked in clear water as a control, and count the germination rate of the seeds in each treatment and measure the radicle length 48 hours after the seeds of the crops in the control show white. Each treatment has 3 repetitions. The results ar...

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Abstract

The invention relates to the field of microbial preparations, in particular to a bacillus inoculant for preventing and treating cucumber fusarium wilt as well as a preparation method and application of the bacillus inoculant. The bacillus fungicide comprises bacillus subtilis ZR-2, and the preservation number of the bacillus subtilis ZR-2 is CGMCC (China General Microbiological Culture Collection Center) NO.21124; and the bacillus amyloliquefaciens ZR-3 has the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO. 21123. The bacillus fungicide can be used for preventing and treating fusarium wilt of cucumbers, can prevent and treat cucumber diseases, can promote germination of cucumber seeds and growth of seedlings, and is beneficial to increase of yield and income of cucumbers.

Description

technical field [0001] The invention relates to the field of microbial preparations, in particular to a bacillus bacterial preparation for preventing and treating cucumber wilt, a preparation method and application thereof. Background technique [0002] Cucumber Fusarium Wilt is one of the important diseases in cucumber production. It is a typical soil-borne disease and occurs all over the country, especially in Jilin, Liaoning, Shandong, Hubei, Beijing and other provinces and cities. The incidence rate is generally 10% to 30%. %, up to 80% to 90% in severe cases, is an important factor restricting the yield and quality of cucumber. In recent years, with the rapid development of greenhouse vegetables in Zhangjiakou area, the multiple cropping index has increased, and the occurrence of cucumber wilt has become more and more serious, causing great losses to cucumber production. Cucumber Fusarium wilt invades from the wound or root tip of the plant root, grows and develops in ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00C12R1/125C12R1/07
CPCC12N1/20A01N63/22
Inventor 李洁明任莉李季吕贻忠
Owner CHINA AGRI UNIV
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