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Multi-path closed bipolar electrochemical luminescence chip and application thereof in detection and sensing

A light-emitting chip and electrochemical technology, applied in the field of microfluidic analysis, can solve the problems of difficult multi-target detection, increase detection error, complicated operation, etc., and achieve the effects of high detection accuracy, increased transfer rate, and high detection efficiency.

Pending Publication Date: 2022-05-13
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Several closed amphoteric electrode anodes correspond to different amphoteric electrode cathodes, and differences in electrode fabrication and modification processes will adversely affect the signal of the amphoteric electrode anode, thereby increasing the detection error
If several amphoteric electrode anodes are located in the same reporting channel, the liquid consumption during detection is large, and it is difficult to perform multi-target detection from different sample sources
If several amphoteric electrode anodes are located in different reporting pools, accurate measurement requires multiple liquid additions, and the operation is complicated

Method used

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  • Multi-path closed bipolar electrochemical luminescence chip and application thereof in detection and sensing
  • Multi-path closed bipolar electrochemical luminescence chip and application thereof in detection and sensing
  • Multi-path closed bipolar electrochemical luminescence chip and application thereof in detection and sensing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] The multi-channel closed bipolar electrochemiluminescent chip (referred to as chip A in this embodiment), its production and detection process is as follows:

[0069] 1. Use the drawing software Adobe Illustrator CS6 to design the configuration of chip A. The carbon electrodes (drive electrodes and closed bipolar electrodes) are made by carbon screen printing technology, and the support channel and report channel are made by solid wax screen printing technology. made.

[0070] The obtained chips are as figure 1 , figure 2 , Figure 5 and Figure 8 As shown, it includes integrated bipolar electrodes, positive 14 and negative 15 driving electrodes, support channel 16 and reporting channels 17-20;

[0071] One end of the integrated bipolar electrode is the bipolar electrode cathode 13;

[0072] The other end of the integrated bipolar electrode is divided into six parallel branches; each parallel branch spans two reporting channels; the part of the parallel branch pla...

Embodiment 2

[0081] The multi-channel closed bipolar ECL chip (called chip B in this embodiment) divides the negative electrode of the driving electrode on the basis of laying chip A, and its manufacturing and detection process is similar to that of chip A.

[0082] 1, Figure 5 It is a schematic diagram of the structure of cloth chip B, which includes bipolar electrode anodes 1-12, bisexual electrode cathodes 13, driving electrode positive electrodes 14, driving electrode negative electrodes 15, support channels 16 and reporting channels 17-20.

[0083] Chip A cannot supply power to the anodes of the bipolar electrodes in each reporting channel separately during detection. In order to make the chip provide more diversified detection modes, it is necessary to individually power the driving electrodes. Dividing the negative electrode of one driving electrode of chip A into four can provide driving voltages for the anodes of the bipolar electrodes in the four reporting channels respectively....

Embodiment 3

[0088] A multi-channel closed bipolar electrochemiluminescent chip (referred to as chip C in this embodiment), on the basis of chip A, the number of bipolar electrode anodes and reporting channels is simplified, and its production and detection process is the same as Chip A is similar.

[0089] 1, Figure 8 It is a structural schematic diagram of chip C, which includes bipolar electrode anodes 1-6, bipolar electrode cathode 13, driving electrode positive 14, driving electrode negative 15, support channel 16, and reporting channels 17 and 18.

[0090] 2. Set up several experimental groups to investigate chip C to simultaneously and accurately detect uric acid and glucose. The concentrations of uric acid and glucose are set to several different concentrations (0mM, 0.1mM, 0.25mM).

[0091]Modified 5 U / μL of glucose oxidase on the anode 1-3 of the bipolar electrode, modified 0.1 U / μL of urate oxidase on the anode 4-6 of the bipolar electrode, and pre-immobilized chitosan and Mu...

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Abstract

The invention discloses a multi-path closed bipolar electrochemical luminescence chip and application thereof in detection and sensing. The chip comprises an integrated bipolar electrode, a driving electrode positive electrode, a driving electrode negative electrode, a support channel and a report channel, one end of the integrated bipolar electrode is a bipolar electrode cathode; the other end of the integrated bipolar electrode is divided into a plurality of parallel branches; the parallel branches stretch across more than one report channel; and the part of the parallel branch, which is arranged in the report channel, is the bipolar electrode anode. In the chip, the series bipolar electrode anodes are connected with the same bipolar electrode cathode, so that the modification process of the bipolar electrode cathode is reduced, and multiple targets from different sample sources can be detected. For the current flowing through the closed bipolar electrode, the magnitude of the current passing through the anode of each bipolar electrode is essentially the same, which is more favorable for examining the light emitting conditions of different areas to evaluate the detection accuracy.

Description

technical field [0001] The invention belongs to the field of microfluidic analysis, in particular to a multi-channel closed bipolar electrochemical luminescence chip and its application in detection and sensing. Background technique [0002] Microfluidic technology aims to integrate sample injection, sample processing, detection and other behaviors on a tiny chip. Compared with traditional detection methods and detection instruments, microfluidic chips have the characteristics of fluid controllability, low consumption of samples and reagents, and fast analysis speed. In recent years, closed amphoteric electrodes combined with electrochemiluminescence have achieved diversified developments, from single detection to high-throughput detection, multiple detection, etc. Closed amphoteric electrodes on microfluidic chips are often designed as different Shape and size to meet different testing needs. Electrochemiluminescent microfluidic chips with closed bipolar electrodes have t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N21/76B01L3/00
CPCG01N27/30B01L3/5027G01N21/76
Inventor 章春笋梁怡
Owner SOUTH CHINA NORMAL UNIVERSITY
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