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A dry bipolar electrochemiluminescence chip and its application in immunoassay

A light-emitting chip and electrochemical technology, which is applied in the direction of chemiluminescence/bioluminescence, electrochemical variables of materials, and analysis by making materials undergo chemical reactions, can solve the problems that biomarkers cannot meet quantitative detection and are difficult to quantitatively detect. Achieve the effect of avoiding micro-environmental influence, easy operation and simple operation

Active Publication Date: 2022-05-03
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technology can effectively overcome the defects of the above-mentioned traditional CBP-ECL microfluidic chip, but it is generally difficult to quantitatively detect
The detection of some biomarkers (cardiac troponin, creatine kinase isoenzyme, myoglobin, etc.) still cannot meet the needs of quantitative detection

Method used

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  • A dry bipolar electrochemiluminescence chip and its application in immunoassay
  • A dry bipolar electrochemiluminescence chip and its application in immunoassay
  • A dry bipolar electrochemiluminescence chip and its application in immunoassay

Examples

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Comparison scheme
Effect test

Embodiment 1

[0057] The utility model relates to a dry bipolar electrochemiluminescence chip, whose composition and structure are as follows: Figure 1-4 As shown in, it includes five parts: sample adding sheet 1, bonding sheet 2, detection sheet 3, electrode sheet 4 and base plate 5.

[0058] The electrode sheet 4（ Figure 4 ）It includes an integrated dual electrode and a driving electrode 7. The dual electrode anode 9 and the corresponding driving electrode are located in the reporting channel 10, and the dual electrode cathode 12 and the corresponding driving electrode are located in the support channel 13; The electrode piece is used to connect with the external circuit and trigger the cbp-ecl reaction. On the one hand, the reporting channel is used to absorb the excess reaction solution on the detection piece, on the other hand, it can be used to connect the CBP current circuit;

[0059] The integrated dual electrode is designed with two anodes 9 distributed in a Y-shape, one of which is pl...

Embodiment 2

[0068] The manufacturing method of the dry bipolar electrochemiluminescence chip of the invention is as follows:

[0069] 1) Configuration design: Adobe Illustrator CS6 drawing software is used to design the configuration of sample sheet 1, binding sheet 2, detection sheet 3 and electrode sheet 4, so as to customize 300 mesh polyester microchannel mesh plate and electrode mesh plate.

[0070] The sample adding sheet, bonding sheet, detection sheet and electrode sheet can be made of paper or cloth fiber materials.

[0071] 2) Chip printing: the front hydrophobic wax dam of sample sheet 1, bonding sheet 2 and detection sheet 3 is made by printing microchannel screen plate with wax screen printing machine (such as cn112192941a). The c-bpe and driving electrode on the front of the electrode sheet 4 are made of the printed electrode screen plate of the silk screen printing machine (model CW 3050, Dongguan Caiwei printing machinery factory), and the hydrophobic wax dam 8 on the back is ...

Embodiment 3

[0080] The application of the dry bipolar electrochemiluminescence chip of the invention in detecting CTN I is as follows:

[0081] 1) Drop the sample solution containing CTN I to be tested on the sample adding tablet 1, the solution quickly flows through the binding tablet 2 and binds with the labeled antibody on it, further flows to the detection tablet 3, and binds with the coated antibody with specific affinity on it to form an immune sandwich complex of "labeled antibody CTN I coated antibody";

[0082] 2) Wait for several minutes, and after the immune reaction of the sample solution to be tested is completed, add buffer to the sample tablet to fill the reporting channel 10 with the solution on the one hand, and remove the unbound labeled antibody in the detection area on the other hand. Then, a buffer is added to the support channel 13 of the electrode sheet 4.

[0083] 3) The driving electrode 7 of the electrode sheet 4 is connected to the set output DC power supply through...

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Abstract

The invention discloses a dry type bipolar electrochemiluminescent chip and its application in immunoassay. The chip includes a bottom plate, an electrode sheet, a detection sheet, a bonding sheet and a sample adding sheet; the electrode sheet includes an integrated bipolar Electrodes and driving electrodes, the integrated bipolar electrode is designed with two anodes distributed in a Y shape, one of the bipolar electrode anodes is placed corresponding to the driving electrode, and is called the detection anode; the electrode sheet is placed in the reporting channel folded between the support channel; the detection sheet is designed with Y-shaped hydrophilic channels; the detection sheet is stacked on the electrode sheet, so that the hydrophilic channel overlaps with the anode of the integrated bipolar electrode; The hydrophilic channel overlapping with the detection anode on the electrode sheet is used as the detection area. This patent is the first invention of a dry immune CBP-ECL chip, which overcomes the shortcomings of the existing immune CBP-ECL chip that is cumbersome to operate and requires multi-step addition of solutions by integrating the reaction reagent components on the chip.

Description

technical field [0001] The invention belongs to the field of microfluidic chip design and detection, in particular to a dry bipolar electrochemiluminescence chip and its application in immune detection. Background technology [0002] In recent years, bisexual electrode (BPE) has been successfully applied in electrochemical synthesis and electrochemiluminescence sensing because of its simple structure and convenient fabrication, which can make the oxidation-reduction reaction of BPE two poles occur simultaneously under the trigger of external electric field. [0003] BPE is divided into open BPE (o-bpe) and closed BPE (c-bpe). The cathode and anode of o-bpe are in the same microchannel, and the reactions at the two poles are easy to affect each other. In addition, the current not only flows through BPE, but also through the solution, the current efficiency is greatly reduced, and the required driving voltage is large. Different from o-bpe, the cathode and anode of c-bpe are in dif...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N27/30G01N33/531B01L3/00
CPCG01N21/76G01N27/307G01N33/531B01L3/5027
Inventor 章春笋占婷婷
Owner SOUTH CHINA NORMAL UNIVERSITY
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