The invention discloses a multi-path closed bipolar electrochemical luminescence chip and application thereof in detection and sensing. The chip comprises an integrated bipolar electrode, a driving electrode positive electrode, a driving electrode negative electrode, a support channel and a report channel, one end of the integrated bipolar electrode is a bipolar electrode cathode; the other end of the integrated bipolar electrode is divided into a plurality of parallel branches; the parallel branches stretch across more than one report channel; and the part of the parallel branch, which is arranged in the report channel, is the bipolar electrode anode. In the chip, the series bipolar electrode anodes are connected with the same bipolar electrode cathode, so that the modification process of the bipolar electrode cathode is reduced, and multiple targets from different sample sources can be detected. For the current flowing through the closed bipolar electrode, the magnitude of the current passing through the anode of each bipolar electrode is essentially the same, which is more favorable for examining the light emitting conditions of different areas to evaluate the detection accuracy.