9 results about "Bipolar electrochemistry" patented technology

The application discloses a dry-type bipolar electrochemiluminescence microfluidic chip and application thereof in protein and nucleic acid detection, wherein the chip comprises a bottom plate, an electrode sheet, a detection sheet, a combination sheet and a sample adding sheet which are stacked in sequence; the integrated bipolar electrode of the electrode sheet is in a "one" shape and has more than one bipolar electrode anode and one bipolar electrode cathode; the multiple bipolar electrode anodes are in a series connection relationship; the number of report channels is consistent with the number of bipolar electrode anodes; the buffer channel and the sample channel on the sample adding sheet correspond to the channel positions on the combination sheet; and the sample channel on the sample adding sheet is one or more. The bipolar electrochemiluminescence microfluidic chip can be used for simultaneous detection of multiple biomarkers in the same sample, and can also be used for simultaneous detection of single or multiple biomarkers in different samples, so as to meet different application requirements in different scenes.

The present invention relates to a method (200) for treating a flow of an aqueous solution, the method comprising: a) activating (205) a particulate medium (20) to be activated, which medium comprises a material having a non-zero electrical conductivity; b) dispensing (210) the thus activated particulate medium (20) into a treatment volume (15); and c) circulating (215) a flow of an aqueous solution to be treated (25) in the treatment volume (15) through the activated particulate medium (20), in order to form a flow of treated aqueous solution (45), which activation takes place using a bipolar electrochemical process, the bipolar electrochemical process comprising the application, using at least two electrodes (95), of an electrical potential difference to an activation compartment (70) containing the particulate medium (20), in the presence of an electrolyte (90), without direct contact between the particulate medium (20) and the electrodes (95).

The invention discloses a split type magnetic attraction bipolar electrochemical luminescence sensor, a detection platform and application of the split type magnetic attraction bipolar electrochemical luminescence sensor. According to the invention, a detection chamber and a signal chamber which are physically separated are constructed, and magnetic control capture and signal transduction of target molecules are realized by combining a magnetic response nickel-coated neodymium iron boron (Ni-coated NdFeB) closed bipolar electrode (mag-BPE); meanwhile, mesoporous silica nanoparticles (MBatMSNs) loaded with methylene blue are used as an electrochemical marker, and the signal response intensity is remarkably improved through the electric neutrality principle of BPE. The invention not only provides a high-sensitivity, economical and simple method for detecting the target object, but also provides a technical reference for the development of a multi-index joint detection platform through the modular design and a universal detection interface, and shows a potential application value in the field of instant diagnosis.

Method and installation for treating a stream of aqueous solution using a particulate medium activated by bipolar electrochemistry. The present invention relates to a method for treating (200) a stream of aqueous solution, the method comprising: a) the activation (205) of a particulate medium (20) to be activated comprising a material having non-zero electrical conductivity, b) the distribution (210) of the particulate medium (20) thus activated in a treatment volume (15), and c) the circulation (215) of a stream of aqueous solution to be treated (25) in the treatment volume (15) through the activated particulate medium (20), to form a treated aqueous solution stream (45), the activation being carried out by a bipolar electrochemistry process.The bipolar electrochemical process comprises applying an electrical potential difference by means of at least two electrodes (95) to an activation compartment (70) containing the particulate medium (20) in the presence of an electrolyte (90), without direct contact between the particulate medium (20) and the electrodes (95). Figure for the abstract: 1,

The application discloses a kind of bipolar electrochemiluminescence microfluidic chip and its application.The bipolar electrochemiluminescence microfluidic chip of the application includes from top to bottom: ITO electrode 1, PDMS chip main body and ITO electrode 2.The application couples horseradish peroxidase (HRP) as signal label with labeled antibody (Ab2), and couples magnetic beads with coated antibody (Ab1).When detecting, only need to inject the reagent coupled with sample to be detected into chip by injection pump at the same time, antibody and detection material AFP are mixed to form immune sandwich complex in chip through spiral microchannel, then are captured on the surface of ITO electrode in detection cavity under the action of electromagnet, and finally electrochemiluminescence signal is output by electric stimulation to realize detection, which can realize fast, simple operation and low-cost detection of AFP, and can be widely applied in screening and diagnosis of HCC.

The invention discloses a dual-mode micro-fluidic chip based on magnetic electrochemistry and bipolar electrochemiluminescence and application of the dual-mode micro-fluidic chip. The dual-mode micro-fluidic chip combines dual-mode signal output of electrochemistry and bipolar electrochemiluminescence. Rapid and reliable detection can be realized. The dual-mode micro-fluidic chip has the advantages that the quasi-homogeneous mixing efficiency is better, and the stability and the selectivity are better; the defect of insufficient accuracy possibly existing in the detection process of a single signal mode is successfully overcome; compared with the prior art, the device has the maintenance-free characteristic, and detection elements do not need to be replaced frequently, so that the operation difficulty during detection is greatly reduced, and the applicability of the device to different environments is also improved.

The invention belongs to the technical field of bipolar electrochemical luminescence sensors, and particularly relates to a closed split magnetic bipolar electrochemical luminescence sensor and application thereof in detection of kaempferol in safflower. According to the bipolar electrochemical luminescence sensor, under the action of driving voltage, kaempferol is subjected to electrochemical oxidation reaction on the surface of the anode of the detection chamber to promote oxygen reduction reaction at the cathode end of the bipolar electrode, so that electrons enriched on the electrode surface of the anode end of the bipolar electrode are increased, and generated Faraday current flows through the bipolar electrode; the electrochemical luminescence behavior of Ru (bpy) 32 + / TPrA (at) Au NPs is triggered in the signal chamber, and the electrochemical luminescence intensity is in direct proportion to the concentration of the kaempferol, so that the detection of the kaempferol is realized. The sensor constructed by the invention has the advantages of low cost, wide linear range, low detection limit, high sensitivity, good stability, good reproducibility and the like, and is a novel kaempferol detection method with extremely high application potential.

The invention provides a method for converting acetylene to produce ethylene by electrically driving a particle electrode in a liquid phase at normal temperature, and belongs to the technical field of ethylene preparation. The method comprises the following steps: adding an organic solvent into an electrolytic tank device with a sealing cover, adding a granular catalyst into the organic solvent, adding an electrolyte, aerating inert gas before reaction, continuously introducing acetylene gas or acetylene-containing ethylene-rich gas into the electrolytic tank filled with the organic solvent, adding a hydrogen source, turning on a power supply of an electrochemical treatment unit, and carrying out electrochemical treatment on the acetylene gas or the acetylene-containing ethylene-rich gas. And carrying out constant-current or constant-potential electrolytic treatment, and after an electrolytic product is constant, realizing the process of producing ethylene by electrochemical conversion of acetylene. According to the method disclosed by the invention, the research on a liquid-phase acetylene electrochemical hydrogenation ethylene production technology is developed by utilizing the solubility difference of acetylene and ethylene and the bipolar electrochemistry and single-particle collision theory, and the conversion from a high-temperature and high-pressure acetylene catalytic hydrogen-adding phase normal-pressure reaction to a normal-temperature and normal-pressure liquid-phase reaction is realized.

The application discloses a flow-based bipolar electrode electrochemiluminescence biological detection method, 1) a centrifugal tube is cut and shaped into a dynamic bipolar electrochemical device; 2) SiO2 doped microbeads with conductivity are prepared; 3) a primary antibody of a to-be-detected marker, an antigen and a secondary antibody labeled with quantum dots are coupled on the SiO2 doped microbeads in sequence to form a biosensor microbead with conductivity; 4) the microbead is placed in a mixed solution containing a co-reagent / buffer, the electrochemical device is placed in a dark box of an electrochemiluminescence detection instrument, the microbead is pushed into the electrochemical device from above through a peristaltic pump; 5) a driving voltage is applied to electrodes at two ends, and an electrochemiluminescence signal generated is recorded through a photomultiplier tube in the dark box. The application first combines flow detection with a traditional electrochemiluminescence detection method, has the characteristics of sustainable flow, high throughput, low background signal and high sensitivity through low-voltage driving, is low in cost and convenient to make.