Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Anti-GPR65 monoclonal antibody, hybridoma cell strain secreting antibody and application

A monoclonal antibody and hybridoma cell technology, applied in the direction of anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, antibody, application, etc., can solve the identification purpose that limits the application field and scope, and cannot be specific Problems such as protein, to achieve the effect of good application prospects

Pending Publication Date: 2022-05-20
SHANDONG ANALYSIS & TEST CENT
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although polyclonal antibodies also play an important role in research, they cannot specifically recognize the target protein, which severely limits its application field and scope

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Anti-GPR65 monoclonal antibody, hybridoma cell strain secreting antibody and application
  • Anti-GPR65 monoclonal antibody, hybridoma cell strain secreting antibody and application
  • Anti-GPR65 monoclonal antibody, hybridoma cell strain secreting antibody and application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0038] Preferably, the preparation method includes the following steps: inject the hybridoma cell GPR65-9G7 described in the third aspect into the peritoneal cavity of an animal for cell proliferation, obtain ascites and perform purification to obtain the monoclonal antibody GPR65-9G7.

[0039] Preferably, the preparation method of the monoclonal antibody hybridoma cell GPR65-9G7 is as follows: construct a GPR65 protein knockout animal, inject the antigen and adjuvant into the animal for immunization, screen the immune positive animals, obtain the organ of the animal that produces the antibody The cells are fused, and positive hybridoma cells are screened to obtain the monoclonal antibody hybridoma cell GPR65-9G7.

[0040] Further, the immunized animals include monkeys, rabbits, dogs, guinea pigs, mice, rats, sheep and goats, preferably mice.

[0041] Further, the antigen is a polypeptide modification shown in SEQ ID NO: 1, and the modification includes coupling with a carrier...

Embodiment 1

[0049] 1. Selection of Immunized Animals

[0050] Since the preparation technology of the mouse monoclonal antibody is very mature, does not involve the issue of patent ownership, is simple to operate, and has low production cost, this example chooses to prepare the monoclonal antibody of the mouse GPR65 protein.

[0051] The mouse GPR65 protein and the human GPR65 protein have high homology, especially the functional region LOOP2 region of GPRs ( https: / / www.uniprot.org / align / A20201028E5A08BB0B2D1C45B0C7BC3B55FD265560019 CC9 ), which brings great difficulties to the preparation of monoclonal antibodies using wild-type mice. Therefore, in this example, the GPR65 protein knockout mouse (GPR65-KO) constructed on the background of C57 / B6 was used to carry out the experiment. When constructing GPR65-KO mice, eGFP protein was inserted. First of all, he helped to identify GPR65-KO.

[0052] (1) DNA was extracted from mouse tissue using a tissue lysis kit. Cut the ear of a mou...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention particularly relates to an anti-monoclonal antibody GPR65-9G7, a hybridoma cell secreting the antibody and application of the anti-monoclonal antibody GPR65-9G7. The GPR65 has expression in various immune cells, and is related to various diseases such as inflammation, multiple sclerosis, thrombocytopenia and tumors. Due to the fact that GPR65 expression and purification difficulty is high, and human source GPR65 protein and mouse source GPR65 protein have high homology, an effective GPR65 monoclonal antibody expression tool does not exist at present. The invention provides the hybridoma cell GPR65-9G7 capable of secreting the GPR65 monoclonal antibody, and the cell strain can secrete the IgG1 subtype GPR65 monoclonal antibody and has a good prospect as a research tool and an immune preparation.

Description

technical field [0001] The invention belongs to the technical field of monoclonal antibody hybridoma cell preparation, and specifically relates to a monoclonal antibody GPR65-9G7, a cell line GPR65-9G7 secreting the monoclonal antibody and applications thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] G-protein-coupled receptors (G Protein-Coupled Receptors, GPCRs) are composed of a series of large superfamily protein receptors with seven transmembrane structures, which can undergo conformational changes under the action of various stimuli, and then Binds to intracellular transducer G protein and other signaling proteins to trigger intracellular signaling cascades....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K16/28C12N15/13C12N5/20G01N33/577G01N33/68A61K39/395A61P35/00
CPCC07K16/28G01N33/577G01N33/68A61P35/00C07K2317/94C07K2317/92A61K2039/505
Inventor 查尔斯·雷伊·麦凯田文静
Owner SHANDONG ANALYSIS & TEST CENT
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com