A kind of Staphylococcus vortexii and its application in bio-enhanced degradation of pesticide wastewater
A technology of staphylococcus and pesticide wastewater, applied in the application field of bioenhanced degradation, to achieve the effects of protecting the ecological environment, preventing groundwater pollution, and protecting human health
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Embodiment 1
[0039] Example 1 Isolation, purification and identification of strains.
[0040] Take 10.0g of soil sample and add it to 100ml of inorganic salt medium containing 100mg / L imazamox. The soil sample comes from the long-term application of the long-residual herbicide imazamox. The soil is placed at 30°C, 150rpm and shaken for 5 days. The liquid was transferred to the inorganic salt medium with the concentration of imazethapyr at 200 mg / L, and after culturing for 7 d under the same conditions, the transfer was continued until the concentration of imazamox in the medium reached 500 mg / L. Then, the bacterial solution was diluted and spread on LB solid medium, and single colonies of different shapes were picked and continued to streak culture until a single colony was obtained. Single colonies of different shapes were selected and cultured in 100 mg / L inorganic salt liquid medium for 21 days. QuEChERS-liquid chromatography-tandem mass spectrometry was used to detect the concentration...
Embodiment 2
[0044] Example 2 Preparation of inoculum.
[0045] A single colony of Staphylococcus vortexii JG-3 was selected and inoculated in a LB medium shake flask, and shaken and cultivated to logarithmic phase; the above-mentioned cultivated strain was inoculated into a seed tank at 10% of the inoculum, and cultivated to logarithmic growth The seed liquid is connected to the production tank at 5% of the inoculum for cultivation, and the medium used in the production tank is the same as that of the seed tank; the ventilation rate of sterile air during the cultivation process of the seed tank and the production tank is 1:0.6- 1.2, the stirring speed is 180-240 rpm, the cultivation temperature is 35 °C, the cultivation time of the whole process is 48-96 hours, and the number of cells reaches 10 after the fermentation. 8 More than 1 / mL, after the fermentation is completed, the culture solution is directly packed into liquid dosage forms in plastic packaging barrels or packaging bottles. ...
Embodiment 3
[0048] Example 3 Degradation effect of strain JG-3 on herbicide intermediates.
[0049] The strain JG-3 of Staphylococcus vortexi that was cultivated to the logarithmic phase in the medium was inoculated with 5% of the inoculum to contain different herbicide intermediates (imazethapyr, imidazolinic acid, 2-methylquinoline). 2,3-quinoline dicarboxylic acid), in the medium, the initial concentration of each herbicide intermediate is 1g / L, NH 4 Cl 0.5g / L, KH 2 PO 4 1g / L, Na 2 HPO 4 1g / L, MgCl 2 0.02g / L, CaCl 2 0.03g / L, pH 7.0-7.5, cultured in a shaker at 30°C and 150r / min. After 24h, determine the content of each herbicide intermediate, such as figure 2 shown. The results showed that Staphylococcus vulgaris had 95.21%, 95.32%, 96.65% and 95.87% of imazamox, imidazolinic acid, 2-methylquinoline and 2,3-quinoline dicarboxylic acid, respectively.
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