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Preparation method of ginkgo leaf extract

A technology for Ginkgo biloba leaves and extracts, which is applied in the field of separation and preparation of Ginkgo biloba extracts by axial compression column, can solve the problems of unreasonable process route, unsuitability for large-scale production, low purity and yield, etc., and achieves elution and purification. The effect is good, the upper column is uniform, and the effect of expanding the scope of clinical application

Pending Publication Date: 2022-06-10
LUNAN PHARMA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It solves the disadvantages of the separation and preparation of Ginkgo biloba extract in the prior art, such as low purity and yield, incomplete removal of impurities, high labor cost, complicated operation, unreasonable process route, and unsuitability for large-scale production.

Method used

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  • Preparation method of ginkgo leaf extract
  • Preparation method of ginkgo leaf extract
  • Preparation method of ginkgo leaf extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Preparation of Ginkgo biloba extract

[0049] A. Take 750 g of ginkgo biloba coarse powder, add 10 and 8 times the amount of 50% ethanol to reflux for extraction twice, 3 hours each time, combine the extracts, stand at 4-6 °C, and cool down to get the cool liquid, filter , concentrated under reduced pressure to obtain concentrated solution I, for subsequent use;

[0050] B. Take concentrated solution I, adjust pH=7.0 with sodium hydroxide solution, add 0.5 times the amount of 95% ethanol solution, stand at 4-6 °C, and cool down to obtain cool liquid;

[0051] C, get step B cooling liquid, filter, add phosphoric acid to adjust pH=5, and concentrate under reduced pressure to obtain concentrated solution II;

[0052] D, get the concentrated solution II of step C, extract and separate with 1.5 times the amount of petroleum ether, remove the petroleum ether layer, obtain the water layer, for subsequent use;

[0053] E. Take the water layer of step D, compress the...

Embodiment 2

[0055] Example 2 Preparation of Ginkgo biloba extract

[0056] A. Take 750 g of ginkgo biloba coarse powder, add 8, 8 and 6 times the amount of 50% ethanol respectively for reflux extraction for 3 times, 1.5 hours each time, combine the extracts, stand at 4-6 ℃, cool down, get chilled liquid, filtered, and concentrated under reduced pressure to obtain concentrated solution I, for subsequent use;

[0057] B, get concentrated solution I, adjust pH=7.5 with potassium bicarbonate solution, add 1 times the amount of 95% ethanol solution, stand at 4-6°C, cool down to obtain cool liquid;

[0058] C, get the cold precipitation liquid of step B, filter, add hydrochloric acid to adjust pH=4.0, and concentrate under reduced pressure to obtain concentrated solution II;

[0059] D, get the concentrated solution II of step C, extract and separate with 2 times the amount of petroleum ether, remove the petroleum ether layer, obtain the water layer, for subsequent use;

[0060] E. Take the w...

Embodiment 3

[0062] Example 3 Preparation of Ginkgo biloba extract

[0063] A. Take 750 g of ginkgo biloba coarse powder, add 8, 8 and 4 times the amount of 49.5% ethanol respectively for reflux extraction for 3 times, 1 hour each time, combine the extracts, stand at 4-6 ℃, and cool down to get a cool liquid , filtered, concentrated under reduced pressure to obtain concentrated solution I, for subsequent use;

[0064] B, get concentrated solution I, adjust pH=8.0 with sodium bicarbonate solution, add 1.5 times the amount of dehydrated ethanol solution, stand at 4-6 ℃ condition, cool down, get cool liquid;

[0065] C, get step B cooling liquid, filter, add hydrochloric acid to adjust pH=4.5, concentrate under reduced pressure, obtain concentrated solution II;

[0066] D, get the concentrated solution II of step C, extract and separate with 1 times the amount of acetone, remove the acetone layer, obtain the water layer, for subsequent use;

[0067] E. Take the water layer of step D, compre...

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Abstract

The invention belongs to the technical field of traditional Chinese medicines, discloses a preparation method of a ginkgo biloba extract, and particularly relates to a method for separating and preparing the ginkgo biloba extract by combining a high-pressure constant-flow sampling pump and an axial compression column filled with macroporous resin. The preparation method comprises the following steps: carrying out organic solvent extraction, alkalifying alcohol precipitation, filtration, extraction, column chromatography and other separation and purification methods on ginkgo leaf coarse powder, and concentrating and drying to obtain the ginkgo leaf extract. According to the preparation method, impurities such as protein, polysaccharide and ginkgolic acid in the ginkgo biloba extract can be effectively removed, the content of effective components such as total flavonol glycosides and total lactones is increased, and the safety and clinical curative effect of the ginkgo biloba extract serving as a raw material for preparation products are guaranteed. Meanwhile, the high-pressure constant-flow sample injection pump is combined with the axial compression column filled with macroporous resin, so that semi-automatic extraction and purification operation can be realized, the production cost is greatly reduced, and the separation and purification effects are good.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, in particular to a method for preparing a ginkgo biloba extract, in particular to a method for separating and preparing a ginkgo biloba extract by using a high-pressure constant-flow sampling pump and an axial compression column equipped with a macroporous resin. method. Background technique [0002] Ginkgo biloba extract is the dry leaf extract of Ginkgo biloba L., a plant of the family Ginkgoaceae, which is light brownish yellow, flowable powder. my country has been using ginkgo biloba as medicine since the Song Dynasty. The 2020 edition of the "Chinese Pharmacopoeia" (hereinafter referred to as "Pharmacopoeia") contains ginkgo biloba, which is sweet, bitter, astringent, and flat in nature. , Astringing the lungs, relieving asthma, resolving turbidity and lowering lipids". [0003] Ginkgo biloba extract mainly contains total flavonol glycosides, terpenoid lactones, polysac...

Claims

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Application Information

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IPC IPC(8): A61K36/16A61K127/00
CPCA61K36/16A61K2236/333A61K2236/39A61K2236/53A61K2236/51A61K2236/55
Inventor 张贵民毕艳艳刘健龙瑞
Owner LUNAN PHARMA GROUP CORPORATION
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