Coating material with modified protein as well as preparation method and application of coating material

A coating material and protein technology, applied in coatings, pharmaceutical formulations, prostheses, etc., can solve problems such as the inability to meet clinical application requirements, and achieve the effects of excellent stability, enhanced thermal stability, and mild conditions.

Pending Publication Date: 2022-06-10
DONGGUAN PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the aging of the population and the increase of trauma among young and middle-aged people, the demand and requirements for biomedical m

Method used

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  • Coating material with modified protein as well as preparation method and application of coating material
  • Coating material with modified protein as well as preparation method and application of coating material
  • Coating material with modified protein as well as preparation method and application of coating material

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

The embodiment of the present invention provides a kind of coating material with modified protein, prepared by the above-mentioned preparation method, modified

S1, carboxyl activation

Carboxyl-containing biological function modification molecule is first activated by carboxyl, and then mixed with protein to obtain modification

Specifically, the specific structure of the carboxyl-containing biological function modification molecule is not limited, it contains carboxyl and can give the material

Wherein, natural biomaterial is selected from plasticity starch-based material (PSM), gelatin (gelatin), collagen

At least one of methylaminopropyl)-3-ethylcarbodiimide hydrochloride and its derivative (EDCI). 1H-benzotriazoles

) direct coupling, since proteins contain multiple amino groups, EDC-mediated cross-linking usually results in the binding of proteins to carboxyl-containing molecules

[0013] The substrate is reacted with a modified protein solution in the presence of an ...

Example Embodiment

S1, carboxyl activation

Grafting DOTA@Cu molecules on bacterial enzymes, then forming a coating, using DOTA@Cu molecules to catalyze the release of nitric oxide from endogenous donors

Concrete steps are as follows:

A. Mix the EDC, NHS and DOTA powders whose molar ratio is 6:6:1, and add the MES buffer solution with pH of 5.6 to make [0058]

B. in above-mentioned liquid, add lysozyme powder to dissolve completely, the final concentration of lysozyme is 1mg / mL, at 37 ℃

C. put the reaction solution into the dialysis bag with a molecular weight of 3500Da for dialysis for 48 hours, change water every 12 hours, get

[0109] D. Prepare a 3 mg / mL aqueous sodium persulfate solution.

[0180] D. Add the modified protein solution on the clean PVC surface and react at 37°C for 8 hours. Wash off the surface

Example Embodiment

2+

The embodiment of the present invention provides a kind of coating material with modified protein, prepared by the above-mentioned preparation method, modified

Concrete steps are as follows:

A. Mix EDC, NHS and heparin sodium powder in a molar ratio of 10:10:1 and add MES buffer solution with pH 5.6

B. in above-mentioned liquid, add lysozyme powder to dissolve completely, the final concentration of lysozyme is 1mg / mL, at 37 ℃

C. put the reaction solution into the dialysis bag with a molecular weight of 7000Da for dialysis for 48 hours, change water every 12 hours, get

[0069] D. Prepare an aqueous sodium persulfate solution of 0.5 mg / mL.

The reaction was carried out for 12 hours. Wash and remove residual protein particles on the surface, and dry with nitrogen to obtain a sample with an anticoagulant and antibacterial surface.

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Abstract

The invention discloses a modified protein coating material as well as a preparation method and application thereof, and relates to the technical field of biological materials. The preparation method of the coating material with the modified protein comprises the following steps: carrying out carboxyl activation on a carboxyl-containing biological function modified molecule, then reacting with amino in the protein, and carrying out effective coupling to form the multifunctional modified protein; under the induction of an oxidizing agent, disulfide bonds maintaining the molecular structure stability in protein molecules break and oxidize to form stable intermediate sulfides, protein structure extension and side chain exposure enable the surface hydrophobicity to be increased, the intermolecular aggregation degree to be increased, meanwhile, the thermal stability is enhanced, and the protein molecules gradually deposit on the surface of a substrate to form a self-assembled coating. The modified protein coating obtained by the method has excellent stability and various biological activities, the process is simple, and the condition is mild.

Description

A kind of coating material with modified protein, its preparation method and application technical field The present invention relates to the technical field of biological materials, in particular, to a kind of coating material with modified protein, its preparation Preparation methods and applications. Background technique Biomaterials are important foundations of biomedical engineering and biotechnology that constitute the basis of modern medicine. At the intersection of science and multidisciplinary fields, biomaterials apply the principles of biology and engineering to the directional composition of materials into furniture Functional materials for living organisms with specific properties and functions for diagnosis, treatment, repair or replacement of human tissues and organs Or improve the function of tissues and organs, protect the lives and health of hundreds of millions of people, improve the quality of human life, and prolong human life. Life. Since th...

Claims

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Application Information

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IPC IPC(8): A61L27/34A61L27/50A61L31/10A61L31/14
CPCA61L27/34A61L27/50A61L31/10A61L31/14A61L2420/02A61L2400/18C08L89/00
Inventor 杨志禄牟小辉施杰源杜泽煜王颖张文泰
Owner DONGGUAN PEOPLES HOSPITAL
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