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Moso bamboo leaf shape regulation gene PheLBD29 and application thereof

A technology for leaf shape and gene regulation, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problem that the function of LBD gene has not been reported yet.

Active Publication Date: 2022-06-24
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the published genes regulating plant growth and development are LBD genes in model plants such as Arabidopsis and rice, but the function of LBD genes in moso bamboo has not been reported yet.

Method used

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  • Moso bamboo leaf shape regulation gene PheLBD29 and application thereof
  • Moso bamboo leaf shape regulation gene PheLBD29 and application thereof
  • Moso bamboo leaf shape regulation gene PheLBD29 and application thereof

Examples

Experimental program
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Embodiment 1

[0023] A Phyllostachys edulis leaf shape regulating gene PheLBD29 of the present invention has the nucleotide sequence shown in SEQ ID NO.1.

[0024] The protein encoded by the Moso bamboo leaf shape regulation gene PheLBD29 of the present invention is the protein described in the following (1) or (2):

[0025] (1) a protein composed of the amino acid sequence of SEQID No.2 in the sequence listing;

[0026] (2) A protein derived from (1) by substituting and / or adding one to ten amino acid residues in the amino acid residue sequence of SEQ ID No. 2 in the sequence listing and having the function of the Phyllostachys pubescens leaf shape regulating gene PheLBD29.

[0027] The application of the Moso bamboo leaf shape regulating gene PheLBD29 in regulating the shape of Arabidopsis thaliana leaves.

[0028] A plant overexpression vector of the present invention is a pCAMBIA1301a-PheLBD29 plant expression vector in which a multiple cloning site region is sequentially connected wit...

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PUM

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Abstract

The invention discloses a moso bamboo leaf shape regulation and control gene PheLBD29 and application thereof. The moso bamboo leaf shape regulation and control gene PheLBD29 has a nucleotide sequence as shown in SEQ ID NO. 1. The invention provides the moso bamboo PheLBD29 gene related to regulation and control of the leaf shape as well as the encoded protein and application thereof for the first time. The PheLBD29 gene overexpression vector is transferred into wild type arabidopsis thaliana through an agrobacterium tumefaciens mediated inflorescence dip dyeing method, and the result shows that compared with a wild type strain, the leaf shape of an overexpression strain is obviously changed. The result provides a theoretical basis for research on moso bamboo leaf development.

Description

technical field [0001] The invention relates to the field of plant molecular biology, in particular to a leaf shape regulating gene PheLBD29 of Phyllostachys pubescens and its application. Background technique [0002] Moso bamboo (Phyllostachys edulis) is a perennial evergreen plant of the Poaceae family, and is the most important bamboo species in China. China is known as the "hometown" of moso bamboo. Phyllostachys pubescens is the economical bamboo species with the largest planting area, the widest distribution range, the fastest growing and the most widely used in China. Phyllostachys pubescens plays a significant role in maintaining water and soil and maintaining the environment. As a wood, it has a very wide range of application value. In addition, bamboo shoots and bamboo leaves have brought important economic benefits to people, occupying a very important position in the production of bamboo industry. [0003] LBD (LATERAL ORGAN BOUNDARIES domain) gene family is a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82C12N1/21C12N15/11A01H5/12A01H6/20C12R1/19
CPCC07K14/415C12N15/8261
Inventor 吴敏项艳兰延钢陈风王康
Owner ANHUI AGRICULTURAL UNIVERSITY
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