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Molecular marker of rice blast resistant gene and application of molecular marker

A rice blast resistance gene and molecular marker technology, applied in genomics, microbial determination/inspection, instruments, etc., can solve problems such as the decline of rice blast resistance ability

Pending Publication Date: 2022-06-28
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Kongyu 131 and Longjing 31 are the main varieties of rice planted in Heilongjiang, but after a long period of planting, the rice blast resistance ability has declined

Method used

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  • Molecular marker of rice blast resistant gene and application of molecular marker
  • Molecular marker of rice blast resistant gene and application of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: Molecular marker screening of rice blast resistance genes

[0061] Instruments: PCR instrument, Qubit fluorescence quantitative instrument, ABI 3730 sequencer.

[0062] Rice gene donor varieties: Yueguang, Kongyu 131 and Longjing 31.

[0063] Molecular marker screening includes the following steps:

[0064] 1) extracting the genomic DNA of the rice plant to be tested;

[0065] 2) using one or more pairs of primers selected from the following table 1, PCR amplification of rice genomic DNA;

[0066] PCR reaction system is calculated as 20μl: 10X PCR reaction buffer 2μ1, 25mM MgSO 4 0.8μl, 2mM dNTP 2μl, 5μM forward primer F and reverse primer R 1.2μl each, genomic DNA 20ng, KOD-Plus polymerase 0.44μl, plus ddH 2 O is made up to 20 μl.

[0067] The PCR reaction conditions were: 94°C pre-denaturation for 2 minutes, 94°C denaturation for 15 seconds, 60°C annealing for 30 seconds, 68°C extension for 1 minute, a total of 35 cycles; 68°C incubation for 5 minutes...

Embodiment 2

[0077] Example 2: Rice blast resistance breeding of Kongyu 131

[0078] With the help of whole genome sequencing analysis, the gene panel established in Example 1 can be applied to rice blast resistance breeding, including the improvement of Heilongjiang main varieties Kongyu 131 and Longjing 31. The blast resistance genes Pi-i, Pi -b, Pi-zt, Pi-z, Pi-kp, km, Pi-a were introduced into the genome of Kongyu 131 and Longjing 31 to improve the resistance of Kongyu 131 and Longjing 31 to rice blast.

[0079] For example, the donor variety containing the rice blast resistance gene is the rice blast resistant Koshihikari variety, and the recipient variety is Kongyu 131. Our goal is to introduce the rice blast resistance gene from the Koshihikari variety into Kongyu 131, to enhance the resistance of Kongyu 131 to rice blast, and to achieve high and stable yield of Kongyu 131.

[0080] 1. Breeding of Kongyu 131 containing the rice blast resistance gene Pi-zt

[0081]First, the Koshih...

Embodiment 3

[0106] Example 3: Rice blast resistance breeding of Longjing 31

[0107] The donor variety containing the rice blast resistance gene is the rice blast resistant Koshihikari variety, and the recipient variety is Longjing 31. Our goal is to introduce the rice blast resistance gene from the Koshihikari variety into Longjing 31 to enhance the resistance of Longjing 31 to rice blast and achieve high and stable rice yield.

[0108] 1. Breeding Longjing 31 containing the rice blast resistance gene Pi-I by screening with molecular markers linked to the rice blast resistance gene

[0109] First, the Koshihikari variety containing the rice blast resistance gene Pi-I was crossed with Longjing 31. Then, the plants containing the Pi-I rice blast resistance gene were screened using molecular markers linked to the rice blast resistance gene. Thereafter, the blast resistant plant was backcrossed with Longjing 31. The purpose of the backcross is to revert its background to Longjing31. In t...

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Abstract

The invention discloses a molecular marker of rice blast resistant genes. The molecular marker is selected from SNP (Single Nucleotide Polymorphism) site sequence pairs of genes panel for screening the rice blast resistant genes Pi-i, Pi-b, Pi-zt, Pi-z, Pi-kp, Pi-km and Pi-a. The molecular marker can be used for guiding rice improvement.

Description

technical field [0001] The invention belongs to the field of gene detection, in particular to a molecular marker of a rice blast resistance gene and its application. Background technique [0002] Rice (Oryza sativa L.) is one of the most important food crops in the world and is eaten by more than half of the world's population. In China, rice cultivation accounts for about 25% of the country's arable land, and its output accounts for about 50% of the country's total grain output. Rice is an important strategic resource for my country's food security and sustainable agricultural development. Rice blast is a worldwide rice disease caused by the fungus Magnaporthe grisea. Rice blast not only reduces the yield of rice, but also reduces the quality of rice. Rice blast seriously damages the yield and quality of rice. [0003] Breeding blast resistant rice varieties is an effective way to control rice blast. Kongyu 131 and Longjing 31 are the main rice varieties in Heilongjiang, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B25/00G16B20/30G16B20/20C12Q1/6895C12Q1/6858C12N15/11
CPCG16B20/30G16B20/20G16B25/00C12Q1/6895C12Q1/6858C12Q2600/156C12Q2600/13C12Q2531/113C12Q2563/107C12Q2565/125
Inventor 韩斌刘晓辉王子轩张弛冯旗翁崎峻
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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