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Strain as well as microecological preparation and application thereof

A technology of bacterial strains and yeast strains, applied in the field of microorganisms, can solve the problems of little research on aquaculture applications, and achieve the effects of bright color, fast reproduction speed, and no side effects

Pending Publication Date: 2022-07-01
GUANGXI UNIV FOR NATITIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of bacterial strain and its microecological preparation and application, to solve the existing bacterial strain most researches that the above-mentioned background technology proposes to focus on promoting aspects such as the carotenoid synthesis of marine red yeast, fermentation process optimization, and A poorly studied problem in aquaculture applications

Method used

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  • Strain as well as microecological preparation and application thereof
  • Strain as well as microecological preparation and application thereof
  • Strain as well as microecological preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1, Screening and identification of Rhododendron B1:

[0046] 1. The colony morphology of strain B1 on the PDA medium is consistent, it is an opaque prototype, the diameter is about 3mm, the colony is pink, the surface is raised, shiny, and the edge is complete (such as figure 1 A), under the scanning electron microscope, the cells of strain B1 are 2-5um in length, spherical, and teething (such as figure 1 B). Use the NCBI website to download the ITS sequence closely related to this strain, and construct a phylogenetic tree with MEGA software (such as figure 2 ).

[0047] 2. Amplification and sequence analysis of 16SrRNA gene:

[0048](1) The fungal genomic DNA extraction was carried out according to the instructions of the kit; (2) The genome of the previous step was used as the template, and ITS primers were used to select general ITS and ITS4; (3) PCR amplification: PCR amplification was carried out in a 50uL system, and the system Components include: PCR...

Embodiment 2

[0054] Embodiment two, the preparation of red yeast inoculum:

[0055] A kind of animal growth promoter is prepared with red yeast fungus, and its preparation method is as follows:

[0056] (1) Red yeast B1, after activation, fermented and cultured at 30°C and 200r / min for 72h to obtain red yeast inoculum;

[0057] (2) Among them, the components of the activated culture medium are: PDA liquid medium: potato 200g, glucose 20g, distilled water 1000mL;

[0058] (3) It was determined that the number of viable bacteria of Rhododendron glabrata in the Rhododendron saccharomyces agent was 3.33X1010CFU / mL.

Embodiment 3

[0059] Embodiment three, the preparation of animal feed additive:

[0060] A feed additive for tilapia is prepared with red yeast fungus, and its preparation method is as follows:

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Abstract

The bacterial strain B1 is classified and named as rhodotorula mucilaginosa, the bacterial colony of the bacterial strain B1 is round, convex, neat in edge, opaque, smooth or relatively smooth in surface and pink in color (about 3 mm), the cell length is 2-5 microns under a scanning electron microscope, and the bacterial strain B1 is spherical and has the cell length of 2-5 microns under a scanning electron microscope. The rhodotorula mucilaginosa provided by the invention is obtained by screening, separating and identifying rhodotorula mucilaginosa in rhizosphere microflora species of a mangrove forest in the north of Guangxi province, and is saprophytic bacteria with relatively strong stress resistance. The feed additive has the effects of promoting the growth of aquatic animals, remarkably improving the oxidation resistance, increasing the survival rate and enhancing the non-specific immunity of organisms.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bacterial strain and its microecological preparation and application. Background technique [0002] In recent years, problems such as pollution of the breeding environment and the abuse of antibiotics in breeding have become increasingly prominent, resulting in a decrease in the quality and safety of breeding animals and affecting human health. There is an urgent need to find and develop natural feeds that are green, safe, non-polluting to the environment, easily absorbed and utilized by animals, and nutritious and healthy. [0003] Red yeast is rich in a variety of amino acids and fatty acids, rich in nutrients, and also contains a variety of bioactive components, such as linolenic acid, linoleic acid and beta-carotene, etc., and can improve the breeding environment. Mangroves contain a large number of microbial planting resources and are a special ecosystem that trans...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/18A23L33/14A61P37/04A61P15/08A61K36/064C12N1/16C12R1/645
CPCA23K50/80A23K10/18A23L33/14C12N1/16A61K36/064A61P15/08A61P37/04A23V2002/00A23V2200/30
Inventor 张文菲王金子姜明国李金秋潘思密
Owner GUANGXI UNIV FOR NATITIES
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