Tree shrew brain nucleus positioning method

Abstract

The invention belongs to the technical field of brain science, and particularly relates to a tree shrew brain nucleus positioning method. The method comprises the following steps: step 1, acquiring a whole-brain MRI map of the brain of the tree shrew with a fowfontano-fowfontano coordinate system as a reference; the fontangle-fontangle coordinate system comprises the position relation among a fontangle, a fontangle, a front tooth bar and an ear line; step 2, performing coordinate correction on the tree shrew brain staining atlas by using the whole-brain MRI atlas obtained in the step 1 to obtain three-dimensional coordinates of a target nucleus; and step 3, navigating or positioning the target nuclei of the brains of the tree shrew experimental animals by using the three-dimensional coordinates obtained in the step 2. The method can be used for related research of tree shrews, is simple and accurate in operation, is convenient for comparison with other animals, and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of brain science, and particularly relates to a method for locating brain nuclei of tree shrews. Background technique [0002] Brain stereotaxic technology is a classic and conventional technical method in the field of brain science research. With the rapid development of brain science and neuroscience in recent years, there is a growing demand for deep brain imaging, in vivo electrophysiology, optogenetics and neural circuit tracking, all of which are based on animal brain nuclei. On the basis of precise positioning of the group. Only when the target nucleus is precisely located, deep brain imaging, intra-brain drug delivery, in vivo electrophysiological recording, optogenetic control, and neural circuit tracking can be performed on this nucleus. [0003] In the prior art, a stereotaxic apparatus is often used in combination with the origin of the coordinates of the animal's brain to accurately navigate a...

AI Technical Summary

Problems solved by technology

The coordinate system of these tree shrews is inconsistent with the general standards of the stereotaxic coordinate system of other model animals, and there are some shortcomings in practical applications, such as the zero point plane is not easy to locate and calibrate, and the operation error is large

However, the difficulty in establishing a tree shrew brain coordinate system that is consistent with the general standards of other model animals' brain stereotaxic coordinate systems is that the brain skull and brain anatomy of tree shrews are quite different from those of rats, mice, and monkeys. It is difficult to find other species as references during the establishment of the coordinate system of the tree shrew brain, providing reference for the construction of its coordinate system

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