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Application of VGF gene in treatment of metastatic prostate cancer

A prostate cancer and anti-prostate cancer technology, applied in the field of biomedicine, can solve problems that have not been reported in the literature, and achieve the effect of inhibiting invasion and metastasis

Pending Publication Date: 2022-07-01
重庆医科大学国际体外诊断研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some studies have shown that the VGF gene is associated with the progression of lung cancer, pancreatic cancer, urothelial cell carcinoma and other cancers. However, so far, the role and molecular mechanism of the VGF gene in the invasion and metastasis of prostate cancer have not been reported in the literature

Method used

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  • Application of VGF gene in treatment of metastatic prostate cancer
  • Application of VGF gene in treatment of metastatic prostate cancer
  • Application of VGF gene in treatment of metastatic prostate cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. TCGA database to investigate the expression level of VGF in prostate cancer.

[0029] Query the expression of VGF in TCGA through the UALCAN cancer database, such as figure 1 shown in A-1B.

[0030] The results showed that VGF was highly expressed in prostate cancer and correlated with its malignancy.

Embodiment 2

[0031] Example 2. Prostate cancer tissue and cell verification.

[0032] Extract the protein from the tissues of patients with benign prostatic hyperplasia and prostate cancer, and detect the expression of VGF in the tissues by Western blot. figure 2 A shown.

[0033] Extract RNA and protein from prostate cells, and detect the expression of VGF in cells by real-time fluorescent quantitative PCR and Western blot. figure 2 B-2C shown.

[0034] 1. Western blot

[0035] (1) Lysis of tissues and cells;

[0036] (2) BCA protein quantification to determine the loading amount and prepare protein samples;

[0037] (3) SDS-polyacrylamide gel electrophoresis and membrane transfer;

[0038] (4) closed;

[0039] (5) Incubate the primary antibody at 4°C overnight;

[0040] (6) Incubate the secondary antibody at room temperature for 1 h;

[0041] (7) ECL luminescence detection.

[0042] 2. Real-time PCR

[0043] (1) The primer sequences are as follows:

[0044] VGF upstream prim...

Embodiment 3

[0051] Example 3. Preparation of VGF inhibitor.

[0052]According to the nucleotide sequence of VGF, siRNA and shRNA of VGF were designed, as shown in SEQ ID NO.1 and SEQ ID NO.2.

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Abstract

The invention belongs to the technical field of biological medicine, and relates to application of a VGF gene in treatment of metastatic prostate cancer. The invention discloses application of a VGF gene inhibitor in preparation of prostatic cancer drugs. It is found for the first time that the VGF can promote invasion and metastasis of the prostate cancer, the VGF has higher expression in prostate cancer tissue with higher malignancy degree, and generation and development of the prostate cancer can be inhibited by inhibiting the VGF. A VGF small-molecule inhibitor siRNA is prepared, so that prostatic cancer is treated, metastasis of the prostatic cancer is inhibited, and a PI3K / AKT signal channel is inhibited. The invention provides a new thought for further researching the pathogenesis of prostatic cancer and the function of the VGF gene, and provides a new direction for the development of prostatic cancer drugs.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to the application of VGF gene in the treatment of metastatic prostate cancer. Background technique [0002] Globally, about half of men with prostate cancer develop metastatic disease and it is one of the most common cancers in men. According to the Cancer Statistics 2022 report, there will be 1,918,030 new cancer cases in the United States in 2022. For men, prostate cancer alone will account for 27% of new cancers, and the number of deaths is second only to lung cancer. With the improvement of the economic level, the improvement of people's living standards and the prolongation of life expectancy, the incidence of prostate cancer has shown a significant upward trend. Most prostate cancer patients in my country have already metastasized when they were first diagnosed. Among cancer patients, 54% of the patients had developed distant metastases (including bone and abdominal organ m...

Claims

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Application Information

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IPC IPC(8): G01N33/574C12Q1/6886C12Q1/6851A61K31/713A61K45/00A61P35/04
CPCG01N33/57484G01N33/57434C12Q1/6886C12Q1/6851A61K45/00A61K31/713A61P35/04C12Q2600/158C12Q2600/112C12Q2531/113C12Q2545/101C12Q2563/107
Inventor 欧俐苹王蕾蕾武莹莹陈婷梅罗春丽
Owner 重庆医科大学国际体外诊断研究院
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