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Sodium alginate/traditional Chinese medicine polysaccharide loaded zinc finger antiviral protein co-assembled medicine and preparation method thereof

A sodium alginate and antiviral technology, which is applied in the direction of peptide/protein components, drug combinations, and pharmaceutical formulations, can solve the problems of antiviral protein loading and release efficiency that are difficult to effectively improve, and achieve selective treatment and comprehensive immunity. The effect of broad application prospects

Pending Publication Date: 2022-07-05
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the polysaccharides of traditional Chinese medicines have certain water solubility, which makes it difficult to effectively improve the loading and release efficiency of zinc finger antiviral proteins.

Method used

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  • Sodium alginate/traditional Chinese medicine polysaccharide loaded zinc finger antiviral protein co-assembled medicine and preparation method thereof
  • Sodium alginate/traditional Chinese medicine polysaccharide loaded zinc finger antiviral protein co-assembled medicine and preparation method thereof
  • Sodium alginate/traditional Chinese medicine polysaccharide loaded zinc finger antiviral protein co-assembled medicine and preparation method thereof

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preparation example Construction

[0031] In the preparation method of the present invention, while the sodium alginate / Chinese medicine polysaccharide self-assembles to form a nano-assembly, the -OH in the Chinese medicine polysaccharide and a large amount of -COOH in the sodium alginate pass through non-covalent hydrogen bonds, π-π stacking, etc. At the same time, the -OH in the traditional Chinese medicine polysaccharide can also co-assemble with the Zn in the zinc finger antiviral protein molecule. 2+ , -NH in cysteine ​​(Cys) and histidine (His) 2 , -COOH, -SH, and imidazole rings generate non-covalent force to realize the loading of zinc finger antiviral protein by sodium alginate / polysaccharide of traditional Chinese medicine. The zinc finger antiviral protein in the present invention is the CCCH type zinc finger antiviral protein (according to the 2+ Different combinations of the conserved amino acid residues cysteine ​​(Cys) and histidine (His) are bound into 9 major classes: C2H2, C8, C6, C3HC4, C2HC...

Embodiment 1

[0042] Dissolve 5 mg of sodium alginate in 1 mL of deionized water and fully dissolve to obtain a solution with a concentration of 5 mg / mL. 10 mg of Atractylodes Rhizoma Polysaccharide was dissolved in 1 mL of deionized water and fully dissolved to obtain a solution with a concentration of 10 mg / mL. The above two solutions were mixed and stirred at 500 rpm / min for 1 h. Add 500 μL of CCCH-ZAP to the above mixture, mix it evenly, add it, and inject 4 mL of 0.1 MCa (NO 3 ) 2 in solution. With the slow addition of sodium alginate / atractylodes polysaccharide-ZAP mixed solution dropwise, the Ca 2+ Under the action of cross-linking, the mixed solution rapidly co-assembled, and after gelation for one hour, the co-assembled drug carrier of sodium alginate / atractylodes polysaccharide-loaded zinc finger antiviral protein was prepared, which realized the loading of zinc finger antiviral protein. . The maximum protein loading rate can reach 46%.

Embodiment 2

[0044] Dissolve 10 mg of sodium alginate in 1 mL of deionized water and fully dissolve to obtain a solution with a concentration of 10 mg / mL. 10 mg of Atractylodes Rhizoma Polysaccharide was dissolved in 1 mL of deionized water and fully dissolved to obtain a solution with a concentration of 10 mg / mL. The above two solutions were mixed and stirred at 500 rpm / min for 1 h. Add 500 μL of CCCH-ZAP to the above mixture, mix it evenly, add it, and inject 4 mL of 0.1 MCa (NO 3 ) 2 in solution. With the slow addition of sodium alginate / atractylodes polysaccharide-ZAP mixed solution dropwise, the Ca 2+ Under the action of cross-linking, the mixed solution rapidly co-assembled, and after gelation for one hour, the co-assembled drug carrier of sodium alginate / atractylodes polysaccharide-loaded zinc finger antiviral protein was prepared, which realized the loading of zinc finger antiviral protein. . The maximum protein loading rate can reach 67%.

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Abstract

The invention discloses a sodium alginate / traditional Chinese medicine polysaccharide loaded zinc finger antiviral protein co-assembled medicine and a preparation method thereof. The preparation method comprises the following steps: dissolving sodium alginate in deionized water to obtain a sodium alginate solution; dissolving traditional Chinese medicine polysaccharide in deionized water to obtain a traditional Chinese medicine polysaccharide solution; dissolving a Ca < 2 + >-containing compound in deionized water to obtain a Ca < 2 + > solution; mixing and stirring the sodium alginate solution and the traditional Chinese medicine polysaccharide solution to obtain a blended solution, adding the zinc finger antiviral protein into the blended solution to obtain a protein solution, dropwise adding the protein solution into the Ca < 2 + > solution, standing, centrifuging and collecting solids to obtain the sodium alginate / traditional Chinese medicine polysaccharide loaded zinc finger antiviral protein co-assembled drug carrier. By utilizing the cross-linking property of sodium alginate in a Ca < 2 + > solution, through the broad-spectrum antibacterial and antiviral activity of traditional Chinese medicine polysaccharide, the immune environment in an animal body is improved, and the specific inhibition of zinc finger antiviral protein on avian leukosis virus is combined, so that the healthy growth of the animal can be finally obviously promoted.

Description

technical field [0001] The invention relates to the technical field of drug carrier preparation, in particular to a sodium alginate / Chinese medicine polysaccharide-loaded zinc finger antiviral protein co-assembled drug and a preparation method thereof. Background technique [0002] Zinc finger antiviral protein (ZAP) is a natural host endogenous immune factor. ZAP can inhibit viral replication by mediating the degradation of viral mRNA and inhibiting translation. Among them, CCCH-type zinc finger antiviral protein (CCCH-ZAP) exhibits specific inhibitory activity against viruses. It has been found that CCCH-type zinc finger antiviral protein can effectively inhibit the replication of leukemia J subgroup virus (ALV-J) as a natural defense factor in chickens. However, the current application of zinc finger antiviral protein is mainly by injection, which is easy to cause stress in livestock and poultry. [0003] In order to improve the problems existing in the administration ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/36A61K38/16A61K31/715A61P35/02
CPCA61K47/36A61K38/16A61K31/715A61P35/02A61K2300/00
Inventor 范海张淑新成子强高现强刘殊君
Owner SHANDONG AGRICULTURAL UNIVERSITY
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