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Protein clusterin related to obesity-related diseases and application of protein clusterin

A protein and disease technology, applied to protein clusterin and its application field, can solve the problems of systemic toxicity of beta-adrenergic receptor agonists, poor operability of cold stimulation, etc.

Pending Publication Date: 2022-07-05
刘丽宏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current methods of activating BAT mainly include cold stimulation and β-adrenergic receptor agonist intervention, but the operability of cold stimulation is poor, and β-adrenergic receptor agonists have problems such as systemic toxicity.

Method used

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  • Protein clusterin related to obesity-related diseases and application of protein clusterin
  • Protein clusterin related to obesity-related diseases and application of protein clusterin
  • Protein clusterin related to obesity-related diseases and application of protein clusterin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Serum CLU levels were significantly elevated in obese patients and positively correlated with BMI

[0034] Non-secondary obesity patients (BMI≥28kg / m) were collected in the early stage 2 ) 28 cases and normal people (18.5≤BMI2 ) Serum samples from 17 cases were detected by ELISA (Human Clusterin ELISA Kit, abcam) to detect the level of CLU protein in serum, such as figure 1 As shown, the CLU level of obese patients (OB) was significantly higher than that of normal subjects (NC); and in obese patients, the serum level of CLU was positively correlated with BMI value (Pearson r=0.57, P<0.01). This result suggests to the inventors that CLU protein may have an important pathological role in obesity.

Embodiment 2

[0035] Example 2: CLU gene knockout protects against high-fat diet-induced obesity

[0036] experimental method

[0037] 2.1 Use 6-week-old male wild-type C57BL / 6N (WT) mice and Clu knockout (provided by Saiye Biotechnology Co., Ltd., Clu KO ) mice were fed a normal diet (CD, fat 10% kcal, carbohydrate 70% kcal, protein 20% kcal) and a high-fat diet (HFD, fat 60% kcal, carbohydrate 20% kcal, protein 20% kcal) 16 weeks (it should be noted that the feed supply is about 6g / mice, but the supply is not equal to the food intake of the mice, and the food intake has statistical data, see Figure 4 a), 5 mice in each group, the body weight of the mice was measured once a week, and the blood glucose of the mice was measured once every 4 weeks. Glucose tolerance (GTT) and fat distribution analysis experiments were performed at 16 weeks.

[0038] (1) Monitoring of body weight and blood sugar: During the experiment, the living conditions of the mice, including coat color, mental state, ...

Embodiment 3

[0044] Example 3: CLU gene knockout can counteract obesity by inhibiting brown fat whitening

[0045] experimental method

[0046] 3.1 Using the mice in 2.2, the metabolic cage experiment was carried out when the mice were raised in high fat for 16 weeks, and then pentobarbital was used to anesthetize them. After blood sampling, they were sacrificed, and the follow-up detection was carried out after sampling.

[0047] (1) Metabolic cage experiment: The experiment was performed using the OxyletPro System (Panlab, Spain). After the mice underwent a 24-h acclimation period, data collection was performed. The activity of the mice was monitored for 24 hours, and the day and night food intake, activity, and hourly oxygen consumption (VO) of the mice were measured. 2 ), carbon dioxide production per hour (VCO 2 ), calculate the mouse respiratory exchange rate (RER=VO 2 / VCO 2 ) and energy consumption.

[0048] (2) HE staining: after the mice were sacrificed, the inguinal white ...

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Abstract

The invention discloses a protein clusterin-clusterin related to obesity-related diseases, and a coding gene CLU (Clusterin) of the protein. The invention further discloses application of the protein clusterin related to the obesity-related diseases and the coding gene of the protein clusterin in preparation of drugs for preventing and treating the obesity-related diseases or kits for detecting the obesity-related diseases. The invention provides a gene or protein cluster related to obesity, and provides a theoretical basis and a new drug target for research and development of obesity drugs.

Description

technical field [0001] The present invention relates to the field of obesity-related disease prevention and treatment. More specifically, the present invention relates to protein clusterins associated with obesity-related diseases and uses thereof. Background technique [0002] Obesity is a chronic metabolic disease characterized by excessive fat accumulation, which can lead to adipose tissue and systemic chronic inflammation, and promote the occurrence of insulin resistance, which can directly lead to or promote the occurrence and development of various diseases such as diabetes and tumors. The report shows that more than half of my country's adult residents are overweight or obese, posing a serious threat to the health of our residents. At present, the main means of treating obesity are lifestyle interventions, drugs or surgery, but none of these interventions can completely solve the problem. [0003] The pathogenesis of obesity is complex and has not yet been fully elu...

Claims

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Application Information

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IPC IPC(8): C07K14/47A61K38/17A61K48/00A61P3/00A61P3/04A61P3/10G01N33/68
CPCC07K14/47A61K38/1709A61K48/005A61P3/04A61P3/00A61P3/10G01N33/6893G01N2333/47G01N2800/042G01N2800/044G01N2800/04G01N2500/04
Inventor 任路路刘丽宏玄玲玲宫丽丽张文
Owner 刘丽宏
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