Real-time fluorescent quantitative PCR (Polymerase Chain Reaction) acquisition system and acquisition method
A real-time fluorescence quantitative and acquisition system technology, applied in the direction of fluorescence/phosphorescence, biochemical equipment and methods, specific-purpose bioreactors/fermenters, etc., can solve the problems of low automation, PCR technology pollution, low detection sensitivity, etc. problems, to achieve the effect of simple operation, high sensitivity and good repeatability
Inactive Publication Date: 2022-07-08
深圳阿斯克医疗有限公司
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[0004] The purpose of the embodiment of this application is to propose a real-time fluorescent quantitative PCR acquisition system and fluorescence acquisition method, which can solve the pollution, low degree of automation and detection of existing PCR technology. low sensitivity problem
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[0029] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field to which this application belongs. The terminology used herein in the specification of the application is for the purpose of describing particular embodiments only and is not intended to limit the application. The terms "comprising" and "having" and any variations thereof in the description and claims of this application and the above description of the drawings are intended to cover non-exclusive inclusions. The terms "first", "second" and the like in the description and claims of the present application or the above drawings are used to distinguish different objects, rather than to describe a specific order.
[0030] Reference herein to an "embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the...
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The invention provides a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) acquisition system, which comprises a fluorescence excitation module used for emitting light rays to a reactant to be detected, so that the light rays excite the reactant to be detected and generate a fluorescence signal with a specific wavelength; the fluorescence acquisition module is connected with the fluorescence excitation light source module and is used for acquiring the fluorescence signal and processing the fluorescence signal to generate fluorescence data; and the processing module is respectively connected with the fluorescence acquisition module and the fluorescence excitation module and is used for processing the fluorescence data and generating fluorescence data waves. Through the mode, the problems of pollution, low automation degree, low detection sensitivity and the like in the existing PCR technology can be solved.
Description
technical field [0001] The present application relates to the technical field of real-time fluorescent quantitative PCR, and in particular, to a real-time fluorescent quantitative PCR acquisition system and a fluorescence acquisition method. Background technique [0002] Real-time fluorescence quantitative PCR (polymerase chain reaction) is an emerging PCR technology, which uses fluorescent dyes or fluorescently labeled specific probes to label and track PCR products, and can monitor the reaction process online in real time. The software can structure the product and calculate the initial concentration of the sample template to be tested, also known as real-time quantitative PCR. [0003] Real-time quantitative PCR refers to the real-time determination of the amount of specific products by continuously monitoring the changes in the intensity of fluorescent signals during the exponential amplification of PCR, and based on this, the initial amount of the target gene is deduced...
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Login to View More IPC IPC(8): C12M1/36C12M1/34C12M1/00G01N21/64
CPCC12Q1/6851B01L7/52G01N21/6428C12Q2531/113C12Q2563/107C12Q2561/113
Inventor 朱卫
Owner 深圳阿斯克医疗有限公司