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Cassava transcription factors MebHLH72 and MebHLH114 and application thereof in inhibition of synthesis of linamarin

A technology of transcription factor, mebhlh72, applied in application, genetic engineering, plant gene improvement, etc., can solve problems such as potential safety hazards

Active Publication Date: 2022-07-08
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existence of CGs in cassava leaves, tubers and their products brings unnegligible safety hazards to the "edible" cassava

Method used

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  • Cassava transcription factors MebHLH72 and MebHLH114 and application thereof in inhibition of synthesis of linamarin
  • Cassava transcription factors MebHLH72 and MebHLH114 and application thereof in inhibition of synthesis of linamarin
  • Cassava transcription factors MebHLH72 and MebHLH114 and application thereof in inhibition of synthesis of linamarin

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Embodiment Construction

[0022] The present invention will be further described below with reference to the accompanying drawings and specific embodiments to better understand the present invention. If no specific technology or condition is indicated in the examples, the technology or condition described in the literature in the field or the product specification is used. The reagents or instruments used without the manufacturer's indication are conventional products that can be obtained from the market.

[0023] 1. Acquisition of cassava transcription factor MebHLH72 gene and MebHLH114 gene

[0024] 1. Acquisition of cassava transcription factor MebHLH72 gene

[0025] Take cassava root cDNA as template, adopt the primers in table 1, the fragment that contains the base sequence 714bp obtained by PCR method amplification (electrophoresis figure is as shown in Fig. figure 1 shown), and its sequence is shown in SEQ ID No: 1. The PCR reaction system is 2×PhantaMax Master Mix 25μl: cDNA 1μl, 10μM primer...

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Abstract

The invention provides an encoding gene of a cassava transcription factor, which is a transcription factor MebHLH72 gene, and the nucleotide sequence of the encoding gene is shown as SEQ ID NO: 1; or the gene is a transcription factor MebHLH114 gene, and the nucleotide sequence of the gene is as shown in SEQ ID NO: 2. The silence system constructed by adopting the segments of the MebHLH72 gene and the MebHLH114 gene can effectively silence a target gene, can effectively regulate and control the expression of a key gene for synthesizing linopicroside in a plant, regulate and control the synthesis of linopicroside in the plant, and regulate the content of cyanoglycoside, for example, after cassava is transfected by the silence system, the expression of the linopicroside in the plant can be effectively regulated and controlled, and the content of the cyanoglycoside in the plant can be regulated and controlled. Relative expression of a key gene for synthesizing cyanoglycoside is reduced to a certain degree, synthesis of linamarin in cassava leaves is obviously inhibited, but synthesis of lotus corniculatus is not obviously influenced, the content of linamarin is obviously reduced, the content of lotus corniculatus is not obviously different, and the content of cyanoglycoside is also obviously reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to cassava transcription factors MebHLH72, MebHLH114 and their application in inhibiting the synthesis of linamarin. Background technique [0002] Cassava (Manihot esculenta Crantz, Cassava) is a perennial plant of the Euphorbiaceae cassava genus. It is also known as the world's three major potato crops along with sweet potato and potato. . There are two cyanogenic glycosides (Cyanogenic Glycosides, CGs) in cassava, Linamarin and Lotaustralin, among which Linamarin accounts for about 95% and is the main cyanogenic glycoside. In cassava, cyanogenic glycosides are first synthesized in the aerial part and then transported to the roots for storage. The cyanogenic glycosides themselves are not toxic. During cooking or processing, linamarinase will degrade part of CGs to produce unstable acetone cyanohydrin, which in turn produces hydrocyanic acid (HCN) and small molecular compounds such as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H5/12A01H6/38
CPCC07K14/415C12N15/8218C12N15/8243Y02A50/30
Inventor 安飞飞肖鑫辉蔡杰薛晶晶罗秀芹陈松笔
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI