Method for extracting hydroxyapatite from fishbone and obtained hydroxyapatite

A hydroxyapatite and fish bone technology, applied in chemical instruments and methods, phosphorus compounds, inorganic chemistry, etc., can solve the problems of low extraction purity of hydroxyapatite and high residual amount of harmful substances, and achieve protein and heavy metal residues reduce production costs, and achieve the effect of popularization and application

Pending Publication Date: 2022-07-12
QINGDAO NAT LAB FOR MARINE SCI & TECH DEV CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical problems of low extraction purity of hydroxyapatite and high residual amount of harmful substances existing in the above-mentioned prior art, and proposes a method for extracting hydroxyapatite from fish bones and the obtained hydroxyapatite

Method used

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  • Method for extracting hydroxyapatite from fishbone and obtained hydroxyapatite

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044]The golden pomfret fish bone waste was added to deionized water and heated and boiled for 1 hour to remove residual fish meat and other impurities on the fish bones, and the obtained product was rinsed for subsequent use; the above-mentioned fish bones were pulverized to 50 meshes; placed in sodium hydroxide (20wt%) solvent, 5 freeze-thaw cycles were performed, the freezing temperature was -80 °C, the freezing time was 12 h, and the thawing time was 3 h at room temperature; then microwave heating was used, the heating temperature was 90 °C, and the heating The time was 1 h to remove the collagen contained in the fish bone; finally, the obtained product was dialyzed to neutrality with deionized water, and freeze-dried to obtain fish bone hydroxyapatite.

Embodiment 2

[0046] The golden pomfret fish bone waste was added to deionized water and heated and boiled for 2 hours to remove residual fish meat and other impurities on the fish bones, and the obtained product was rinsed for subsequent use; the above-mentioned fish bones were pulverized to 150 mesh; placed in sodium hydroxide (12wt%) solvent, perform 3 freeze-thaw cycles, the freezing temperature is -30°C, the freezing time is 8h, and the thawing time is 1h at room temperature; then microwave heating is used, the heating temperature is 70°C, and the heating The time is 10h to remove the collagen contained in the fish bone; finally the obtained product is dialyzed to neutrality with deionized water, and freeze-dried to obtain fish bone hydroxyapatite.

Embodiment 3

[0048] The golden pomfret fish bone waste was added to deionized water and heated and boiled for 3 hours to remove residual fish meat and other impurities on the fish bones, and the obtained product was rinsed for subsequent use; the above-mentioned fish bones were pulverized to 300 meshes; placed in sodium hydroxide (10wt%) solvent, perform 2 freeze-thaw cycles, the freezing temperature is 0 °C, the freezing time is 3 h, and the thawing time is 2 h at room temperature; then microwave heating is used, the heating temperature is 60 °C, and the heating time is 2 h. For 24h, the collagen contained in the fish bone was removed; the final product was dialyzed to neutrality with deionized water, and freeze-dried to obtain fish bone hydroxyapatite.

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Abstract

The invention provides a method for extracting hydroxyapatite from fish bones, which comprises the following steps: adding the fish bones into deionized water, heating to boil, removing residues on the fish bones, and washing the fish bones for later use; crushing the cleaned fish bones to obtain fish bone powder; putting the fish bone meal into a sodium hydroxide, lithium hydroxide or potassium hydroxide solvent, and performing freezing-unfreezing circulation treatment for multiple times; carrying out heating treatment on the fish bones subjected to repeated freezing-unfreezing circulation treatment; and dialyzing the product with deionized water until the product is neutral, and freeze-drying to obtain hydroxyapatite. According to the method disclosed by the invention, the collagen fibers in the fish bones can be rapidly separated, the residual quantity of proteins and heavy metals in the obtained hydroxyapatite is obviously reduced, and the purity is high.

Description

technical field [0001] The invention belongs to the field of hydroxyapatite preparation, and particularly relates to a method for extracting hydroxyapatite from fish bones and the obtained hydroxyapatite. Background technique [0002] Hydroxyapatite is a functional calcium-containing inorganic salt, which has been widely used in chemical industry, biomedicine, food and other fields because of its good biological and chemical activity. At present, the common hydroxyapatite on the market is mostly prepared by chemical synthesis method, and its structure and chemical properties are difficult to meet the application requirements in special fields. [0003] Therefore, more and more researchers have begun to pay attention to the natural hydroxyapatite synthesized by the organism itself, and fish bone is one of its important sources. The bones of fish are mainly composed of collagen and hydroxyapatite. The hydroxyapatite synthesized by its own metabolism has many advantages such a...

Claims

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Application Information

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IPC IPC(8): C01B25/32
CPCC01B25/327C01P2006/80C01P2002/72
Inventor袁世鹏毕世超谭永明牛晶晶
OwnerQINGDAO NAT LAB FOR MARINE SCI & TECH DEV CENT