Bile duct cancer organoid culture medium and culture method

Abstract

The invention discloses a bile duct cancer organoid culture medium and a culture method. Clinical tissue samples from bile duct cancer patients are used for constructing bile duct cancer organoid, currently, the cultured bile duct cancer organoid can grow massively and rapidly and can be stably passed, and bile duct cancer organoid markers are identified; mainly through adjustment of components and concentrations of a separation medium and an amplification medium in a bile duct cancer organ culture system in the early stage, related signal channels comprise a Wnt signal channel activator, a tyrosine receptor kinase ligand for stimulating epithelial cell proliferation, a TGF-beta signal channel inhibitor for inhibiting epithelial differentiation, a TGF-beta signal channel inhibitor for inhibiting epithelial cell proliferation, a TGF-beta signal channel inhibitor for inhibiting epithelial cell proliferation and a TGF-beta signal channel inhibitor for inhibiting epithelial cell proliferation. And the ROCK inhibitor Y-27632 can maintain the pluripotency of the stem cells.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a cholangiocarcinoma organoid culture medium and a culture method. Background technique [0002] Cholangiocarcinoma (CCA) refers to cancer originating from any segment of the small intrahepatic bile duct to the lower end of the common bile duct, including the primary tumor of the small intrahepatic bile duct, left and right hepatic ducts, common hepatic duct, common bile duct and the end of the bile duct. Malignant tumors arising in the diverse biliary tree. CCA is usually asymptomatic in its early stages, so it is often diagnosed when the disease is already in an advanced stage, which greatly affects treatment options, resulting in a poor prognosis. [0003] CCA is a rare cancer, but its morbidity and mortality, considering the global increase in incidence over the past few decades, represent a global health problem. Despite advances in CCA awareness, knowledge, diagnosis, and trea...

AI Technical Summary

Problems solved by technology

Due to the high heterogeneity and severe fibrosis of cholangiocarcinoma, the construction of cholangiocarcinoma organoids in China is still in the exploratory stage. After 2-3 months of culture, it is prone to growth stagnation, difficulty in passage, and difficulty in surviving after recovery. Therefore, the method of organoid culture in cholangiocarcinoma still needs to be further improved.

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