Artificial hatching method for barbus capito roes
A technology for artificial hatching and fish eggs, applied in fish farming, application, animal husbandry, etc., can solve the problems of reducing the success rate of fish eggs hatching, fish eggs hypoxia, etc.
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Embodiment 1
[0025] 1. Experiment setup method
[0026] Control treatment: put water fleas in purified water at a density of 50 / L, and in the test, adjust the pH of the water body according to the required pH value of the water body (pH=6.5, 7, 7.5, 8, 8.5), the killing rate of Daphnia daphnia in the water body was counted after 24 hours.
[0027] Treatment A1: Adjust the pH of the purified water (pH=6.5, 7, 7.5, 8, 8.5) with sodium hydroxide and acetic acid solution according to the required pH value of the water body, and then add 15g / L of tourmaline to the water body After adding tourmaline, 24 hours after the tourmaline was put in, the water fleas were put into the water at a density of 50 / L, and the killing rate of the water fleas in the water was calculated after 24 hours.
[0028] Treatment A2: Fill the center of hollow plastic microspheres with tourmaline to obtain a density of about 0.8g / cm 3 Adjust the pH of the purified water (pH=6.5, 7, 7.5, 8, 8.5) with sodium hydroxide and ...
Embodiment 2
[0049] In the present embodiment, the method for obtaining the fertilized roe of the four barb carps that is adopted is as follows:
[0050] 1. Breeding selection
[0051] The parents are collected from the wild in the Lancang River and cultivated through domestication and selection.
[0052] 2. Breeding of broodstock
[0053] (1) Construction of a cultivation pond: the bottom of the pond is flat, with less silt, with an area of 0.2m 2 , the water depth is 1.5m;
[0054] (2) Male-female ratio and stocking amount: the male-female ratio is 1:1.5, and the stocking density is 80 / 100m 2 , the breeding pond can be matched with stocking fish that are concerned about feeding;
[0055] (3) Feed and feeding: put artificial compound feed, supplemented with grass, the daily feed is 1% of the fish weight, and the composition of the compound feed is protein 30%, fat 3.5%, crude fiber 11%, coarse ash powder 15% , lysine 1.3%.
[0056] 3. Artificial reproduction
[0057] The artifici...
Embodiment 3
[0074] In this example, according to the method for obtaining fertilized fish eggs of S. chinensis described in Example 2, the fertilized eggs of S. chinensis were obtained, and 5 groups of treatment groups were set, which were marked as treatment C1, treatment C2, treatment C3, treatment C4, processing C5, the setting method of each processing group is as follows.
[0075] Treatment C1: Before hatching the fertilized fish eggs, soak the fertilized fish eggs for pretreatment; for the first soaking, place the fertilized fish eggs in 25 μL / L H 2 O 2 Soak in the aqueous solution for 15 minutes, and drain the soaking solution; for the second soaking, place the fertilized fish eggs in a phosphate buffer (pH=7.6) containing 15g / L lecithin for 15 minutes, and drain the soaking solution; The fertilized eggs of the above-mentioned pre-soak are placed in a ring-shaped water tank for incubation. During the incubation process, the water temperature of the ring-shaped water tank is kept i...
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