Ear cartilage cell, preparation method, regenerated ear cartilage tissue and application

Abstract

The invention discloses an auricular cartilage cell and a preparation method thereof, a regenerated auricular cartilage tissue and application, and the preparation method of the auricular cartilage cell comprises the following steps: step 1, respectively taking cartilage tissues at an auricular tip, an auricular center and an auricular root, and respectively cutting into pieces; 2, the ear tip cartilage powder obtained in the step 1 is placed in an I-type collagenase solution with the concentration being 2-3 g / L for digestion, the ear center cartilage powder is placed in an I-type collagenase solution with the concentration being 4-6 g / L for digestion, the ear root cartilage powder is placed in an I-type collagenase solution with the concentration being 6-8 g / L for digestion, the solid-liquid mass-volume ratio in digestive juice is 0.08-0.12 g / mL, and the digestion time is 2-3 h; respectively obtaining ear tip cartilage digestive juice, ear center cartilage digestive juice and ear root cartilage digestive juice; and 3, mixing the auricular cartilage digestive juice, the auricular central cartilage digestive juice and the auricular cartilage digestive juice obtained in the step 2, filtering tissue residues through a 40-micron cell filter, centrifuging filtrate, removing supernatant, and re-suspending cells, so that the yield of the auricular central and auricular cartilage cells can be remarkably improved.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to an ear cartilage cell and a preparation method, regenerated ear cartilage tissue and application. Background technique [0002] Ear chondrocytes are important seed cells in tissue-engineered ear reconstruction. They are planted on scaffolds constructed of synthetic materials, secrete cartilage extracellular matrix, and finally form mature cartilage tissue, which can be used for microtia reconstruction, ear cartilage trauma repair, etc. Reduced iatrogenic damage caused by autologous cartilage tissue transplantation. At the same time, ear chondrocytes can also be used as tool cells for the study of the pathogenesis of microtia. However, the content of cells in cartilage tissue is only 5%-10%, which is a small cell tissue, mainly composed of extracellular matrix such as collagen, glycosaminoglycan, and elastic fibers. During the extraction of ear chondrocytes, the e...

AI Technical Summary

Problems solved by technology

During the extraction of ear chondrocytes, the cells are exposed to digestive enzymes for a long time while degrading the extracellular matrix, which not only reduces the number of extracted cells, but also reduces the cell viability

In addition, we found that the content of extracellular matrix components in different parts of the ear cartilage tissue, and the force of the extracellular matrix vary greatly. If the traditional unified collagenase concentration is used for digestion, the extraction efficiency of the ear root and the center of the ear will be reduced, resulting in tissue damage. Waste, and prolonging the digestion time will have a great impact on cell viability, reducing cell yield and viability

Images