Ear cartilage cell, preparation method, regenerated ear cartilage tissue and application

A chondrocyte and cartilage tissue technology, applied in the field of biomaterials, can solve problems such as reducing cell viability, tissue waste, and reducing the number of extracted cells, and achieves the effects of avoiding cell activity, increasing yield, and increasing secretion

Inactive Publication Date: 2022-07-29
PLASTIC SURGERY HOSPITAL CHINESE ACAD OF MEDICAL SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the extraction of ear chondrocytes, the cells are exposed to digestive enzymes for a long time while degrading the extracellular matrix, which not only reduces the number of extracted cells, but also reduces the cell viability
In addition, we found that the content of extracellular matrix components in different parts of the ear cartilage tissue, and the force of the extracellular matrix vary greatly. If the traditional unified collagenase concentration is used for digestion, the extraction efficiency of the ear root and the center of the ear will be reduced, resulting in tissue damage. Waste, and prolonging the digestion time will have a great impact on cell viability, reducing cell yield and viability

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ear cartilage cell, preparation method, regenerated ear cartilage tissue and application
  • Ear cartilage cell, preparation method, regenerated ear cartilage tissue and application
  • Ear cartilage cell, preparation method, regenerated ear cartilage tissue and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Step 1: Take the ear tip cartilage tissue of the pig's auricle, and cut it into pieces to approximately 1mmx1mmx1mm to obtain the ear tip cartilage fragments;

[0034] Step 2: Place the ear tip cartilage fragments obtained in step 1 in a type II collagenase solution with a concentration of 2 g / L for digestion, wherein the solid-liquid mass-volume ratio (referring to the ear tip cartilage fragments and type II collagen) in the digested solution The volume-to-mass ratio of the enzyme solution) was 0.1 g / mL, and the digestion conditions were all incubated at 37°C on a shaker for 10 h to obtain the ear tip cartilage digestion solution;

[0035] Step 3: Pass the ear tip cartilage digestion solution obtained in Step 2 through a 40 μm cell strainer to filter the tissue residue, centrifuge the filtrate, remove the supernatant, and resuspend the cells at 5*10 5 / cm 2 densities were grown in cell culture dishes.

[0036] Wherein, the cartilage tissue in the step 1 is the cartil...

Embodiment 2

[0039] The same as Example 1, the difference is that the tissue taken in this example is the central ear cartilage tissue of pig auricle, and after shredding, the central ear cartilage is obtained, and the type II collagenase solution corresponding to the central ear cartilage is obtained. The concentration is 6g / L.

Embodiment 3

[0041] With Example 1, the difference is that the tissue taken in this example is the ear root cartilage tissue of pig auricle, and after shredding, the ear root cartilage fragments are obtained, and the concentration of the type II collagenase liquid corresponding to the ear root cartilage fragments is: 8g / L.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an auricular cartilage cell and a preparation method thereof, a regenerated auricular cartilage tissue and application, and the preparation method of the auricular cartilage cell comprises the following steps: step 1, respectively taking cartilage tissues at an auricular tip, an auricular center and an auricular root, and respectively cutting into pieces; 2, the ear tip cartilage powder obtained in the step 1 is placed in an I-type collagenase solution with the concentration being 2-3 g / L for digestion, the ear center cartilage powder is placed in an I-type collagenase solution with the concentration being 4-6 g / L for digestion, the ear root cartilage powder is placed in an I-type collagenase solution with the concentration being 6-8 g / L for digestion, the solid-liquid mass-volume ratio in digestive juice is 0.08-0.12 g / mL, and the digestion time is 2-3 h; respectively obtaining ear tip cartilage digestive juice, ear center cartilage digestive juice and ear root cartilage digestive juice; and 3, mixing the auricular cartilage digestive juice, the auricular central cartilage digestive juice and the auricular cartilage digestive juice obtained in the step 2, filtering tissue residues through a 40-micron cell filter, centrifuging filtrate, removing supernatant, and re-suspending cells, so that the yield of the auricular central and auricular cartilage cells can be remarkably improved.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to an ear cartilage cell and a preparation method, regenerated ear cartilage tissue and application. Background technique [0002] Ear chondrocytes are important seed cells in tissue-engineered ear reconstruction. They are planted on scaffolds constructed of synthetic materials, secrete cartilage extracellular matrix, and finally form mature cartilage tissue, which can be used for microtia reconstruction, ear cartilage trauma repair, etc. Reduced iatrogenic damage caused by autologous cartilage tissue transplantation. At the same time, ear chondrocytes can also be used as tool cells for the study of the pathogenesis of microtia. However, the content of cells in cartilage tissue is only 5%-10%, which is a small cell tissue, mainly composed of extracellular matrix such as collagen, glycosaminoglycan, and elastic fibers. During the extraction of ear chondrocytes, the e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/077A61L27/38
CPCC12N5/0655A61L27/3817A61L27/3852C12N2509/00C12N2509/10A61L2430/14A61L2430/40
Inventor 张薇蒋海越
Owner PLASTIC SURGERY HOSPITAL CHINESE ACAD OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap